دورية أكاديمية
أعرض في EDS

Reference genes for RT-qPCR analysis in Musa acuminata genotypes contrasting in resistance to Fusarium oxysporum f. sp. cubense subtropical race 4.

التفاصيل البيبلوغرافية
العنوان: Reference genes for RT-qPCR analysis in Musa acuminata genotypes contrasting in resistance to Fusarium oxysporum f. sp. cubense subtropical race 4.
المؤلفون: de Castro Costa É; Departamento de Fitopatologia, Universidade de Brasília, Instituto de Ciências Biológicas, Campus Universitário Darcy Ribeiro, Brasília, DF, 70910-900, Brazil., Bastos LS; Departamento de Fitopatologia, Universidade de Brasília, Instituto de Ciências Biológicas, Campus Universitário Darcy Ribeiro, Brasília, DF, 70910-900, Brazil., Gomes TG; Departamento de Biologia Celular, Universidade de Brasília, Instituto de Ciências Biológicas, Campus Universitário Darcy Ribeiro, Brasília, DF, 70910-900, Brazil., Miller RNG; Departamento de Biologia Celular, Universidade de Brasília, Instituto de Ciências Biológicas, Campus Universitário Darcy Ribeiro, Brasília, DF, 70910-900, Brazil. robertmiller@unb.br.
المصدر: Scientific reports [Sci Rep] 2024 Jul 17; Vol. 14 (1), pp. 16578. Date of Electronic Publication: 2024 Jul 17.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Fusarium*/genetics , Musa*/microbiology , Musa*/genetics , Plant Diseases*/microbiology , Plant Diseases*/genetics , Real-Time Polymerase Chain Reaction*/methods , Disease Resistance*/genetics , Genotype*, Gene Expression Regulation, Plant ; Genes, Plant ; Reference Standards ; Gene Expression Profiling/methods
مستخلص: Banana (Musa spp.) is the most widely consumed fruit globally. Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense (Foc), is a highly threatening disease to banana production. Resistance genes to Foc exist in wild Musa genotypes such as Musa acuminata subsp. burmannicoides var. Calcutta 4. Whilst real-time PCR (RT-qPCR) is appropriate for accurate analysis of gene expression in pathways involved in host defence responses, reference genes with stable expression under specific biotic stress conditions and host tissue types are necessary for normalization of sample variation. In this context, the stability in potential host reference genes ACT1, APT, EF1α, GAPDH, αTUB, RAN, UBIQ1, UBIQ2, βTUB1, βTUB3, L2 and ACTA1 was evaluated in total RNA samples from root tissues in Calcutta 4 (resistant) and Musa sp. cultivar Prata-anã (susceptible) extracted during interaction with Foc subtropical race 4 (STR4). Expression stability was calculated using the algorithms geNorm, NormFinder and BestKeeper. βTUB3 and L2 were identified as the most stable in Calcutta 4, with ACTA1 and GAPDH the most stable in Prata-anã. These reference genes for analysis of gene expression modulation in the Musa-Foc STR4 pathosystem are fundamental for advancing understanding of host defence responses to this important pathogen.
(© 2024. The Author(s).)
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معلومات مُعتمدة: 00193. 00000778/2021-03 Fundação de Apoio à Pesquisa do Distrito Federal; 00193. 00000778/2021-03 Fundação de Apoio à Pesquisa do Distrito Federal; 00193. 00000778/2021-03 Fundação de Apoio à Pesquisa do Distrito Federal; 00193. 00000778/2021-03 Fundação de Apoio à Pesquisa do Distrito Federal; 465480/2014-4 Instituto Nacional de Ciência e Tecnologia - Plant Stress Biotech; 465480/2014-4 Instituto Nacional de Ciência e Tecnologia - Plant Stress Biotech; 465480/2014-4 Instituto Nacional de Ciência e Tecnologia - Plant Stress Biotech; Finance Code 001 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Finance Code 001 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; 308165/2021-7 Conselho Nacional de Desenvolvimento Científico e Tecnológico; 308165/2021-7 Conselho Nacional de Desenvolvimento Científico e Tecnológico
فهرسة مساهمة: Keywords: Fusarium oxysporum; Musa; Gene expression normalization
SCR Organism: Fusarium oxysporum
تواريخ الأحداث: Date Created: 20240717 Date Completed: 20240717 Latest Revision: 20240720
رمز التحديث: 20240720
مُعرف محوري في PubMed: PMC11255279
DOI: 10.1038/s41598-024-67538-0
PMID: 39020014
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-024-67538-0