دورية أكاديمية
Protium heptaphyllum essential oil from the fruit as a sedative and anesthetic in Rhamdia quelen: influence in cardiac frequency, biochemical, and oxidative parameters.
| العنوان: | Protium heptaphyllum essential oil from the fruit as a sedative and anesthetic in Rhamdia quelen: influence in cardiac frequency, biochemical, and oxidative parameters. |
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| المؤلفون: | da Silva HNP; Institute of Water Science and Technology of Federal, University of Western Pará, Pará, Santarém, Brazil., Dornelles JL; Department of Physiology and Pharmacology, Federal University of Santa Maria, Rio Grande Do Sul, Santa Maria, Brazil., Bandeira Junior G; Department of Physiology and Pharmacology, Federal University of Santa Maria, Rio Grande Do Sul, Santa Maria, Brazil., Barany A; Department of Biology, Faculty of Marine and Environmental Sciences, ExcelenciaInternacional del Mar (CEI MAR), Instituto Universitario de Investigación Marina (INMAR), University of Cádiz, Campus de, 11519, Cádiz, Puerto Real, Spain.; Department of Genetics, Physiology and Microbiology, Faculty of Biological Sciences, Complutense University of Madrid, 28040, Madrid, Spain., da Silva LVF; Institute of Water Science and Technology of Federal, University of Western Pará, Pará, Santarém, Brazil., Mourão RHV; Institute of Collective Health, Federal University of Western Pará, Pará, Brazil., Baldissera MD; Department of Biomedicine, Universidade Franciscana (UFN), Santa Maria, Rio Grande Do Sul, Brazil., de Freitas Milarch C; Faculdade IELUSC, R. Mafra, 84, Joinville, Santa Catarina, 89221-665, Brazil., Portela VM; Department of Physiology and Pharmacology, Federal University of Santa Maria, Rio Grande Do Sul, Santa Maria, Brazil., Martínez-Rodríguez G; Consejo Superior de Investigaciones Científicas (ICMAN-CSIC), Instituto de Ciencias Marinas de Andalucía, 11519, Cádiz, Puerto Real, Spain., Mancera JM; Department of Biology, Faculty of Marine and Environmental Sciences, ExcelenciaInternacional del Mar (CEI MAR), Instituto Universitario de Investigación Marina (INMAR), University of Cádiz, Campus de, 11519, Cádiz, Puerto Real, Spain., Martos-Sitcha JA; Department of Biology, Faculty of Marine and Environmental Sciences, ExcelenciaInternacional del Mar (CEI MAR), Instituto Universitario de Investigación Marina (INMAR), University of Cádiz, Campus de, 11519, Cádiz, Puerto Real, Spain., Baldisserotto B; Department of Physiology and Pharmacology, Federal University of Santa Maria, Rio Grande Do Sul, Santa Maria, Brazil. bernardo.baldisserotto@ufsm.br. |
| المصدر: | Fish physiology and biochemistry [Fish Physiol Biochem] 2024 Jul 18. Date of Electronic Publication: 2024 Jul 18. |
| Publication Model: | Ahead of Print |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Kluwer Academic Publishers Country of Publication: Netherlands NLM ID: 100955049 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-5168 (Electronic) Linking ISSN: 09201742 NLM ISO Abbreviation: Fish Physiol Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Dordrecht ; Boston : Kluwer Academic Publishers Original Publication: Amsterdam ; Berkeley : Kugler, 1986- |
| مستخلص: | This study aimed to evaluate the effects of Protium heptaphyllum fruit essential oil (PHEO) on the physiology of silver catfish (Rhamdia quelen) during anesthesia and recovery, through studying echocardiograms, oxidative status, and metabolic parameters. Three experiments were performed: (1) 50 silver catfish juveniles were submitted to anesthesia and recovery tests with 300, 400, 500, 600, and 700 mg L -1 of PHEO. (2) Echocardiogram analysis was performed in anesthetized and non-anesthetized fish. (3) Biochemical parameters were evaluated at 0, 30, 60, and 120 min of recovery after being anesthetized for 3 min with 600 mg L -1 PHEO. Times to sedation and deep anesthesia were reduced with PHEO increasing concentrations. The echocardiogram showed a higher cardiac rate in anesthetized fish. Plasma glucose levels increased in control fish through recovery time, but anesthetized fish showed lower levels than controls at 120 min of recovery. Metabolic parameters such as plasma and hepatic glucose did not show changes considering the recovery time of up to 120 min. Hepatic glycogen, lactate, and triglycerides reduced their levels over recovery times. Fish anesthetized enhanced superoxide dismutase activity and thiobarbituric acid reactive substances levels but decreased reduced glutathione (GSH) levels at 30 min compared to controls. After 60 min, GSH values were significantly higher in anesthetized fish than in controls. These results suggest that PHEO at 600 mg L -1 is an effective anesthetic for the rapid handling of silver catfish, providing stable metabolic parameters and enhanced antioxidant responses during recovery. Echocardiogram analysis confirms the anesthetic effect, supporting PHEO as a viable and efficient option for fish anesthesia in aquaculture. The use of PHEO in aquaculture can enhance fish welfare by reducing stress during handling and transportation, potentially leading to improved growth, health, and survival rates. (© 2024. The Author(s), under exclusive licence to Springer Nature B.V.) |
| References: | Aydın B (2022) Anaesthetic efficacy of eugenol in doctor fish (Garra rufa): behavioural and cardiovascular responses. Aquac Res 53:6419–6429. https://doi.org/10.1111/are.15049. (PMID: 10.1111/are.15049) Aydın B, Orhan N (2021) Effects of thymol and carvacrol anesthesia on the electrocardiographic and behavioral responses of the doctor fish Garra rufa. Aquaculture 533:736134. https://doi.org/10.1016/j.Aquaculture.2020.736134. (PMID: 10.1016/j.Aquaculture.2020.736134) Baldissera MD, Gonçalves RA, Sagrillo MR, Grando TH, Ritter CS, Grotto FS, Brum GF, Da Luz SCA, Silveira SO, Fausto VP, Boligon AA, Vaucher RA, Stefani LM, Da Silva AS, Souza CF, Monteiro SG (2016) Effects of treatment with the anti-parasitic drug diminazene aceturate on antioxidant enzymes in rat liver and kidney. Naunyn Schmiedebergs Arch Pharmacol 389:429–438. https://doi.org/10.1007/s00210-016-1212-z. (PMID: 10.1007/s00210-016-1212-z26809354) Barbas LAL, Hamoy M, Mello VJ, Barbosa RPM, Lima HST, Torres MF, Nascimento LAS, Silva JKDR, Andrade EHDA, Gomes MRF (2017) Essential oil of citronella modulates electrophysiological responses in tambaqui Colossoma macropomum: a new anaesthetic for use in fish. Aquaculture 479:60–68. https://doi.org/10.1016/j.aquaculture.2017.05.027. (PMID: 10.1016/j.aquaculture.2017.05.027) Batista ED, Brandao FR, Majolo C, Inoue LAKA, Maciel PO, Oliveira MR, Chaves FCM, Chagas EC (2018) Lippia alba essential oil as anesthetic for tambaqui. Aquaculture 495:545–549. (PMID: 10.1016/j.aquaculture.2018.06.040) Bhagat J, Ingole B, Singh N (2016) Glutathione S-transferase, catalase, superoxide dismutase, glutathione peroxidase, and lipid peroxidation as biomarkers of oxidative stress in snails: A review. Invertebr Surviv J 13:336–349. Braun N, Lima RL, Dalla Flora F, Lang ME, Bauermann LF, Loro VL, Baldisserotto B (2008) Lipid peroxidation and superoxide dismutase activity in silver catfish (Rhamdia quelen) juveniles exposed to different dissolved oxygen levels. Cienc Anim Bras 9(3):811–814. https://doi.org/10.5216/cab.v9i3.4944. (PMID: 10.5216/cab.v9i3.4944) Brijs J, Sandblom E, Axelsson M, Sundell K, Sundh H, Huyben D, Broström R, Kiessling A, Berg C, Gräns A (2018) The final countdown: continuous physiological welfare evaluation of farmed fish during common aquaculture practices before and during harvest. Aquaculture 495:903–911. https://doi.org/10.1016/j.aquaculture.2018.06.081. (PMID: 10.1016/j.aquaculture.2018.06.081) Castanhêde SM, Amado LL, Costa BMPA, Barbas LAL, Torres MF, Hamoy AO, Paz CA, Ferreira CBS, Lima GO, Sousa JR, Almeida LCN, Bentes NHM, Mello VJ, Hamoy M (2021) Menthol exposure induces reversible cardiac depression and reduces lipid peroxidation in the heart tissue of tambaqui Colossoma macropomum. Aquaculture 541:736847. https://doi.org/10.1016/j.aquaculture.2021.736847. (PMID: 10.1016/j.aquaculture.2021.736847) Chowdhury S, Saikia SK (2020) Oxidative stress in fish: a review. J Sci Res 12:145–160. https://doi.org/10.3329/jsr.v12i1.41716. (PMID: 10.3329/jsr.v12i1.41716) Cotter PA, Rodnick KJ (2006) Differential effects of anesthetics on electrical properties of the rainbow trout (Oncorhynchus mykiss) heart. Comp Biochem Physiol Part A Mol Integ Physiol 145:158–165. https://doi.org/10.1016/j.cbpa.2006.06.001. (PMID: 10.1016/j.cbpa.2006.06.001) Cunha MA, Barros FMC, Oliveira Garcia L, Lima Veeck AP, Heinzmann BM, Loro VL, Emanuelli T, Baldisserotto B (2010) Essential oil of Lippia alba: a new anesthetic for silver catfish, Rhamdia quelen. Aquaculture 306:403–406. https://doi.org/10.1016/j.aquaculture.2010.06.014. (PMID: 10.1016/j.aquaculture.2010.06.014) Cunha JA, Scheeren CA, Salbego J, Gressler LT, Madaloz LM, Bandeira Junior G, Bianchini AE, Pinheiro CG, Bordignon SAL, Heinzmann BM, Baldisserotto B (2017) Essential oils of Cunila galioides and Origanum majorana as anesthetics for Rhamdia quelen: efficacy and effects on ventilation and ionoregulation. Neotrop Ichthyol 15(1):e160076. https://doi.org/10.1590/1982-0224-20160076. (PMID: 10.1590/1982-0224-20160076) Ellis T, Yildiz HY, López-Olmeda J, Spedicato MT, Tort L, Øverli Ø, Martins CI (2012) Cortisol and finfish welfare. Fish Physiol Biochem 38:163–188. https://doi.org/10.1007/s10695-011-9568-y. (PMID: 10.1007/s10695-011-9568-y22113503) Ferreira AL, Santos FAC, Souza AD, Favero GC, Pinheiro CG, Heinzmann BM, Baldisserotto B, Luz RK (2022) Anesthetic and sedative efficacy of essential oil of Hesperozygis ringens and the physiological responses of Oreochromis niloticus after biometric handling and simulated transport. Fish Physiol Biochem 48(5):1155–1166. https://doi.org/10.1007/s10695-022-01109-w. (PMID: 10.1007/s10695-022-01109-w35932409) Ferreira AL, Souza AD, Santos FAC, Pinheiro CG, Favero GC, Heinzmann BM, Baldisserotto B, Luz RK (2023) Hesperozygis ringens essential oil as an anesthetic for Colossoma macropomum during biometric handling. Cienc Rural 53(9):e220220264. https://doi.org/10.1590/0103-8478cr220220264. (PMID: 10.1590/0103-8478cr220220264) Garlet QI, Souza CF, Rodrigues P, Descovi SN, Martinez-Rodríguez G, Baldisserotto B, Heinzmann BM (2019) GABAa receptor subunits expression in silver catfish (Rhamdia quelen) brain and its modulation by Nectandra grandiflora Nees essential oil and isolated compounds. Behav Brain Res 376:112178. https://doi.org/10.1016/j.bbr.2019.112178. (PMID: 10.1016/j.bbr.2019.11217831454673) Gonçalves JC, Oliveira FS, Benedito RB, Sousa DP, Almeida RN, Araújo DA (2008) Antinociceptive activity of (-)-carvone: evidence of association with decreased peripheral nerve excitability. Biol Pharm Bull 31:1017–1020. (PMID: 10.1248/bpb.31.101718451538) Gondim ANS, Lara A, Santos-Miranda A, Roman-Campos D, Lauton-Santos S, Menezes-Filho JER, Vasconcelos CML, Conde-Garcia EA, Guatimosim S, Cruz JS (2017) (-)-Terpinen-4-ol changes intracellular Ca 2+ handling and induces pacing disturbance in rat hearts. Eur J 807:56–63. https://doi.org/10.1016/j.ejphar.2017.04.022. (PMID: 10.1016/j.ejphar.2017.04.022) González-Burgos E, Gómez-Serranillos MP (2012) Terpene compounds in nature: a review of their potential antioxidant activity. Cur Med Chem 19:5319–5341. (PMID: 10.2174/092986712803833335) Gressler LT, Parodi TV, Riffel APK, Dacosta ST, Baldisserotto B (2012) Immersion anaesthesia with tricaine methanesulphonate or propofol on different sizes and strains of silver catfish Rhamdia quelen. J Fish Biol 81:1436–1445. https://doi.org/10.1111/j.1095-8649.2012.03409.x. (PMID: 10.1111/j.1095-8649.2012.03409.x22957883) Hajek GJ, Klyszejko B (2004) The effects of propiscin (etomidate) on the behaviour, heart rate, and ventilation of common carp. Cyprinus Carpio l Acta Ichthyol Piscatoria 34(2):129–143. (PMID: 10.3750/AIP2004.34.2.02) Huang WC, Hsieh YS, Chen IH, Wang CH, Chang HW, Yang CC, Ku TH, Yeh SR, Chuang YJ (2010) Combined use of MS-222 (tricaine) and isoflurane extends anesthesia time and minimizes cardiac rhythm side effects in adult zebrafish. Zebrafish 7:297–304. https://doi.org/10.1089/zeb.2010.0653. (PMID: 10.1089/zeb.2010.065320807039) Jerez-Cepa I, Ruiz-Jarabo I (2021) Physiology: an important tool to assess the welfare of aquatic animals. Biology 10:61. https://doi.org/10.3390/biology10010061. (PMID: 10.3390/biology10010061334675257830356) Joyce W, Warwicker J, Shields HA, Perry SF (2023) Evolution and divergence of teleost adrenergic receptors: why sometimes ‘the drugs don’t work’ in fish. J Exp Biol 226:jeb245859. https://doi.org/10.1242/jeb.245859. (PMID: 10.1242/jeb.24585937823524) Keppler D, Decker K (1974) Glycogen determination with amyloglucosidase. Methods Enzym Anal 3:1127–1131. Misra HP, Fridovich I (1972) The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 247:3170–3175. https://doi.org/10.1016/S0021-9258(19)45228-9. (PMID: 10.1016/S0021-9258(19)45228-94623845) Nóbrega FF, Salvadori MG, Masson CJ, Mello CF, Nascimento TS, Leal-Cardoso JH, Sousa DP, Almeida RN (2014) Monoterpenoid terpinen-4-ol exhibits anticonvulsant activity in behavioural and electrophysiological studies. Oxid Med Cell Longev 2014(1):703848. (PMID: 251800694142302) Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358. https://doi.org/10.1016/0003-2697(79)90738-3. (PMID: 10.1016/0003-2697(79)90738-336810) Parodi TV, Cunha MAD, Becker AG, Zeppenfeld CC, Martins DI, Koakoski G, Barcellos LG, Heinzmann BM, Baldisserotto B (2014) Anesthetic activity of the essential oil of Aloysia triphylla and effectiveness in reducing stress during transport of albino and gray strains of silver catfish, Rhamdia quelen. Fish Physiol Biochem 40:323–334. (PMID: 10.1007/s10695-013-9845-z23974669) Ray S, Sinha R (2014) Serum cortisol and glucose: reliable bioindicators of stress in the fish Labeo rohita. J Innov 1:6–17. Reid SG, Bernier NJ, Perry SF (1998) The adrenergic stress response in fish: control of catecholamine storage and release. Comp Biochem Physiol Part C Pharmacol Toxicol Endocrinol 120:1–27. Saccol EMH, Uczay J, Pês TS, Finamor IA, Ourique GM, Riffel APK, Schmidt D, Caron BO, Heinzmann BM, Llesuy SF, Lazzari R, Baldisserotto B, Pavanato MA (2013) Addition of Lippia alba (Mill) N. E. Brown essential oil to the diet of the silver catfish: an analysis of growth, metabolic and blood parameters and the antioxidant response. Aquaculture 416–417:244–254. https://doi.org/10.1016/j.aquaculture.2013.09.036. (PMID: 10.1016/j.aquaculture.2013.09.036) Saccol EMH, Toni C, Pês TS, Ourique GM, Gressler LT, Silva LVF, Mourão RHV, Oliveira RB, Baldisserotto B, Pavanato MA (2017) Anaesthetic and antioxidant effects of Myrcia sylvatica (G. Mey.) DC. and Curcuma longa L. essential oils on tambaqui (Colossoma macropomum). Aquac Res 48:2012–2031. https://doi.org/10.1111/are.13034. (PMID: 10.1111/are.13034) Saccol EMH, Jerez-Cepa I, Ourique GM, Pês TS, Gressler LT, Mourão RHV, Martínez-Rodríguez G, Mancera JM, Baldisserotto B, Pavanato MA, Martos-Sitcha JA (2018) Myrcia sylvatica essential oil mitigates molecular, biochemical and physiological alterations in Rhamdia quelen under different stress events associated to transport. Res Vet Sci 117:150–160. https://doi.org/10.1016/j.rvsc.2017.12.009. (PMID: 10.1016/j.rvsc.2017.12.00929275218) Santos AC, Bandeira Junior B, Zago DC, Zeppenfeld CC, Silva DT, Heinzmann BM, Baldisserotto B, Cunha MA (2017) Anesthesia and anesthetic action mechanism of essential oils of Aloysia triphylla and Cymbopogon flexuosus in silver catfish (Rhamdia quelen). Vet Anaesth Analg 44:106–113. https://doi.org/10.1111/vaa.12386. (PMID: 10.1111/vaa.1238627216232) Sarrazin S, Silva L, Assunção A, Oliveira R, Calao V, Silva R, Stashenko E, Maia J, Mourão R (2015) Antimicrobial and seasonal evaluation of the carvacrol-chemotype oil from Lippia origanoides Kunth. Molecules 20(2):1860–1871. (PMID: 10.3390/molecules20021860256256816272275) Sedlak J, Lindsay RH (1968) Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem 25:192–205. https://doi.org/10.1016/0003-2697(68)90092-4. (PMID: 10.1016/0003-2697(68)90092-44973948) Sharma S, Gupta J, Prabhakar PK, Gupta P, Solanski P, Rajput A (2019) Phytochemical repurposing of natural molecule: sabinene for identification of novel therapeutic benefits using in silico and in vitro approaches. Assay Drug Dev Technol 17(8):339–351. https://doi.org/10.1089/adt.2019.93. (PMID: 10.1089/adt.2019.9331846362) Silva ERD, Oliveira DRD, Melo MDFF, Bizzo HR, Leitão SG (2016) Report on the Malungo expedition to the Erepecuru river, Oriximiná, Brazil. Part I: is there a difference between black and white breu? Rev Bras Farmacog 26:647–656. https://doi.org/10.1016/j.bjp.2016.05.003. (PMID: 10.1016/j.bjp.2016.05.003) Silva HNP, Carvalho BCF, Maia JLDS, Becker AG, Baldisserotto B, Heinzmann BM, Mourao RHV, Silva LVF (2019) Anesthetic potential of the essential oils of Lippia alba and Lippia origanoides in tambaqui juveniles. Cienc Rural 49:e20181059. https://doi.org/10.1590/0103-8478cr20181059. (PMID: 10.1590/0103-8478cr20181059) Silva HNP, Machado SDDS, Siqueira AMDA, Silva ECC, Canto MADO, Jensen L, Silva LVF, Fugimura MMS, Barroso ADS, Mourão RHV, Baldisserotto B (2020) Sedative and anesthetic potential of the essential oil and hydrolate from the fruit of Protium heptaphyllum and their isolated compounds in Colossoma macropomum juveniles. Aquaculture 529:735629. https://doi.org/10.1016/j.aquaculture.2020.735629. (PMID: 10.1016/j.aquaculture.2020.735629) Slaninova A, Smutna M, Modra H, Svobodova Z (2009) A review: oxidative stress in fish induced by pesticides. Neuro Endocrinol Lett 30:2. (PMID: 20027135) Small BC (2003) Anesthetic efficacy of metomidate and comparison of plasma cortisol responses to tricaine methanesulfonate, quinaldine and clove oil anesthetized channel catfish. Aquaculture 218:177–185. https://doi.org/10.1016/S0044-8486(02)00302-2. (PMID: 10.1016/S0044-8486(02)00302-2) Soengas JL (2014) Contribution of glucose- and fatty acid sensing systems to the regulation of food intake in fish. A Review Gen Comp Endocrinol 205:36–48. https://doi.org/10.1016/j.ygcen.2014.01.015. (PMID: 10.1016/j.ygcen.2014.01.01524530522) Souza CDF, Baldissera MD, Salbego J, Lopes JM, Vaucher RDA, Mourão RHV, Caron BO, Heinzmann BM, Silva LVFD, Baldisserotto B (2017) Physiological responses of Rhamdia quelen (Siluriformes: Heptapteridae) to anesthesia with essential oils from two different chemotypes of Lippia alba. Neotrop Ichthyol 15:e160083. https://doi.org/10.1590/1982-0224-20160083. (PMID: 10.1590/1982-0224-20160083) Souza CF, Baldissera MD, Silva LDL, Geihs MA, Baldisserotto B (2018a) Is monoterpene terpinen-4-ol the compound responsible for the anesthetic and antioxidant activity of Melaleuca alternifolia essential oil (tea tree oil) in silver catfish? Aquaculture 486:217–223. https://doi.org/10.1016/j.aquaculture.2017.12.025. (PMID: 10.1016/j.aquaculture.2017.12.025) Souza CF, Baldissera MD, Bianchini AE, Silva EG, Mourão RHV, Silva LVF, Schmidt D, Heinzmann BM, Baldisserotto B (2018b) Citral and linalool chemotypes of Lippia alba essential oil as anesthetics for fish: a detailed physiological analysis of side effects during anesthetic recovery in silver catfish (Rhamdia quelem). Fish Physiol Biochem 44:21–34. https://doi.org/10.1007/s10695-017-0410-z. (PMID: 10.1007/s10695-017-0410-z) Souza ASL, Peret AC, Hamoy M, Souza RAL, Torres MF, Barbas LAL (2019a) Propofol and essential oil of Nepeta cataria induce anaesthesia and marked myorelaxation in tambaqui Colossoma macropomum: implications on cardiorespiratory responses. Aquaculture 500:160–169. https://doi.org/10.1016/j.aquaculture.2018.10.017. (PMID: 10.1016/j.aquaculture.2018.10.017) Souza CDF, Baldissera MD, Baldisserotto B, Heinzmann BM, Martos-Sitcha JA, Mancera JM (2019) Essential oils as stress-reducing agents for fish aquaculture: a review. Front Physiol 10:785. https://doi.org/10.3389/fphys.2019.00785. (PMID: 10.3389/fphys.2019.00785312812646596282) Sun P, Zhang Y, Yu F, Parks E, Lyman A, Wu Q, Ai L, Hu CH, Zhou Q, Shung K (2009) Micro-electrocardiograms to study post-ventricular amputation of zebrafish heart. Ann Biomed Eng 37:890–901. https://doi.org/10.1007/s10439-009-9668-3. (PMID: 10.1007/s10439-009-9668-3192803416991467) Tavares-Dias M, Araújo C, Porto S, Viana G, Monteiro P (2013) Sanidade do tambaqui Colossoma macropomum nas fases de larvicultura e alevinagem. Embrapa Amapá-Documentos (INFOTECA-E). https://www.infoteca.cnptia.embrapa.br/bitstream/doc/961527/1/AP2013Documentos78.pdf. Valenti WC, Barros HP, Moraes-Valenti P, Bueno GW, Cavalli RO (2021) Aquaculture in Brazil: past, present and future. Aquac Rep 19:100611. https://doi.org/10.1016/j.aqrep.2021.100611. (PMID: 10.1016/j.aqrep.2021.100611) |
| معلومات مُعتمدة: | process 19/2551-0000655-1 Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS/CAPES- Brazil); process 19/2551-0000655-1 Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS/CAPES- Brazil); PID2020-117557RB-C22 MCIN/AEI/10.13039/501100011033 and by the European Union; process 406615/2018-7 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil) |
| فهرسة مساهمة: | Keywords: Anesthesia; Metabolism; Oxidative status; Recovery; Silver catfish |
| تواريخ الأحداث: | Date Created: 20240717 Latest Revision: 20240717 |
| رمز التحديث: | 20240718 |
| DOI: | 10.1007/s10695-024-01380-z |
| PMID: | 39020065 |
| قاعدة البيانات: | MEDLINE |
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| DOI: | 10.1007/s10695-024-01380-z |