دورية أكاديمية
Is FSH combined with equine chorionic gonadotropin able to modify lipid metabolism in bovine superstimulated antral follicles?
| العنوان: | Is FSH combined with equine chorionic gonadotropin able to modify lipid metabolism in bovine superstimulated antral follicles? |
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| المؤلفون: | Santos PH; Departamento de Biofísica e Farmacologia, Instituto de Biociências, Universidade Estadual Paulista 'Júlio de Mesquita Filho', Botucatu, SP, Brasil., Franchi FF; Departamento de Biofísica e Farmacologia, Instituto de Biociências, Universidade Estadual Paulista 'Júlio de Mesquita Filho', Botucatu, SP, Brasil., Nunes SG; Departamento de Biofísica e Farmacologia, Instituto de Biociências, Universidade Estadual Paulista 'Júlio de Mesquita Filho', Botucatu, SP, Brasil., Fontes PK; Departamento de Biofísica e Farmacologia, Instituto de Biociências, Universidade Estadual Paulista 'Júlio de Mesquita Filho', Botucatu, SP, Brasil., Giroto AB; Departamento de Ciência Animal, Universidade do Oeste Paulista, Presidente Prudente, SP, Brasil., Mani F; Departamento de Química e Bioquímica, Instituto de Biociências, Universidade Estadual Paulista 'Júlio de Mesquita Filho', Botucatu, SP, Brasil., Castilho ACS; Departamento de Ciência Animal, Universidade do Oeste Paulista, Presidente Prudente, SP, Brasil. |
| المصدر: | Animal reproduction [Anim Reprod] 2024 Jul 05; Vol. 21 (2), pp. e20230063. Date of Electronic Publication: 2024 Jul 05 (Print Publication: 2024). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Colégio Brasileiro de Reprodução Animal Country of Publication: Brazil NLM ID: 101272804 Publication Model: eCollection Cited Medium: Internet ISSN: 1984-3143 (Electronic) Linking ISSN: 18069614 NLM ISO Abbreviation: Anim Reprod Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Belo Horizonte, MG, Brazil : Colégio Brasileiro de Reprodução Animal, [2004]- |
| مستخلص: | Lipid metabolism is essential for ensuring oocyte maturation and embryo development. β-Oxidized fatty acids (FA) are a potent source of energy for cells, particularly for bovine somatic follicular cells. Superstimulatory protocols using follicle stimulating hormone (FSH) or FSH combined with equine chorionic gonadotropin (eCG) are capable of stimulating the follicular microenvironment and drive the expression of biomarker genes associated with lipid metabolism in the cumulus-oocyte complex (COC) for better embryo development. In this study, we assesed the effects of FSH and FSH/eCG protocols on the expression of genes related to lipid metabolism in bovine granulosa cells (GCs). Further, we measured triglyceride levels in follicular fluid (FF) obtained from both superstimulatd and non-superstimulated cows (synchronized cows). In summary, superstimulation with gonadotropins maintained the TG levels in bovine FF and ensured GCs mRNA abundance of ACSL1, ACSL3, ACSL6, SCD, ELOVL5, ELOVL6, FASN, FADS2, and SREBP1. We, however, found the abundance of CPTIB mRNA to be lower in GCs obtained from cows subjected to FSH/eCG protocols than synchronized cows. In conclusion, the findings of this study showed that ovarian superstimulation around the preovulatory phase has a mild impact on the lipid metabolism in GCs. Competing Interests: Conflicts of interest: The authors have no conflict of interest to declare. (Copyright © The Author(s).) |
| فهرسة مساهمة: | Keywords: bovine; follicle microenvironment; gene expression; superovulation; triglycerides |
| تواريخ الأحداث: | Date Created: 20240718 Latest Revision: 20240718 |
| رمز التحديث: | 20240718 |
| مُعرف محوري في PubMed: | PMC11253788 |
| DOI: | 10.1590/1984-3143-AR2023-0063 |
| PMID: | 39021495 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1984-3143 |
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| DOI: | 10.1590/1984-3143-AR2023-0063 |