دورية أكاديمية
أعرض في EDS

Healthy and diabetic primary human osteoblasts exhibit varying phenotypic profiles in high and low glucose environments on 3D-printed titanium surfaces.

التفاصيل البيبلوغرافية
العنوان: Healthy and diabetic primary human osteoblasts exhibit varying phenotypic profiles in high and low glucose environments on 3D-printed titanium surfaces.
المؤلفون: Allen N; Duke University Medical Center, Duke University, Durham, NC, United States., Aitchison AH; Duke University Medical Center, Duke University, Durham, NC, United States., Abar B; Duke University Medical Center, Duke University, Durham, NC, United States., Burbano J; Duke University Medical Center, Duke University, Durham, NC, United States., Montgomery M; Duke University Medical Center, Duke University, Durham, NC, United States., Droz L; Duke University Medical Center, Duke University, Durham, NC, United States., Danilkowicz R; Duke University Medical Center, Duke University, Durham, NC, United States., Adams S; Duke University Medical Center, Duke University, Durham, NC, United States.
المصدر: Frontiers in endocrinology [Front Endocrinol (Lausanne)] 2024 Jul 03; Vol. 15, pp. 1346094. Date of Electronic Publication: 2024 Jul 03 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101555782 Publication Model: eCollection Cited Medium: Print ISSN: 1664-2392 (Print) Linking ISSN: 16642392 NLM ISO Abbreviation: Front Endocrinol (Lausanne) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Lausanne : Frontiers Research Foundation]
مواضيع طبية MeSH: Titanium*/pharmacology , Osteoblasts*/metabolism , Printing, Three-Dimensional* , Glucose*/metabolism , Glucose*/pharmacology , Diabetes Mellitus, Type 2*/metabolism , Diabetes Mellitus, Type 2*/pathology, Humans ; Cells, Cultured ; Male ; Phenotype ; Surface Properties ; Female ; Middle Aged ; Bone Morphogenetic Protein 7/metabolism ; Osteogenesis/drug effects ; Collagen Type I/metabolism ; Collagen Type I/genetics ; Collagen Type I, alpha 1 Chain/metabolism ; Collagen Type I, alpha 1 Chain/genetics ; Aged
مستخلص: Background: The revolution of orthopedic implant manufacturing is being driven by 3D printing of titanium implants for large bony defects such as those caused by diabetic Charcot arthropathy. Unlike traditional subtractive manufacturing of orthopedic implants, 3D printing fuses titanium powder layer-by-layer, creating a unique surface roughness that could potentially enhance osseointegration. However, the metabolic impairments caused by diabetes, including negative alterations of bone metabolism, can lead to nonunion and decreased osseointegration with traditionally manufactured orthopedic implants. This study aimed to characterize the response of both healthy and diabetic primary human osteoblasts cultured on a medical-grade 3D-printed titanium surface under high and low glucose conditions.
Methods: Bone samples were obtained from six patients, three with Type 2 Diabetes Mellitus and three without. Primary osteoblasts were isolated and cultured on 3D-printed titanium discs in high (4.5 g/L D-glucose) and low glucose (1 g/L D-Glucose) media. Cellular morphology, matrix deposition, and mineralization were assessed using scanning electron microscopy and alizarin red staining. Alkaline phosphatase activity and L-lactate concentration was measured in vitro to assess functional osteoblastic activity and cellular metabolism. Osteogenic gene expression of BGLAP , COL1A1 , and BMP7 was analyzed using reverse-transcription quantitative polymerase chain reaction.
Results: Diabetic osteoblasts were nonresponsive to variations in glucose levels compared to their healthy counterparts. Alkaline phosphatase activity, L-lactate production, mineral deposition, and osteogenic gene expression remained unchanged in diabetic osteoblasts under both glucose conditions. In contrast, healthy osteoblasts exhibited enhanced functional responsiveness in a high glucose environment and showed a significant increase in osteogenic gene expression of BGLAP , COL1A1 , and BMP7 (p<.05).
Conclusion: Our findings suggest that diabetic osteoblasts exhibit impaired responsiveness to variations in glucose concentrations, emphasizing potential osteoblast dysfunction in diabetes. This could have implications for post-surgery glucose management strategies in patients with diabetes. Despite the potential benefits of 3D printing for orthopedic implants, particularly for diabetic Charcot collapse, our results call for further research to optimize these interventions for improved patient outcomes.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2024 Allen, Aitchison, Abar, Burbano, Montgomery, Droz, Danilkowicz and Adams.)
References: Antioxid Redox Signal. 2019 Oct 1;31(10):687-709. (PMID: 31250671)
Curr Rev Musculoskelet Med. 2021 Feb;14(1):1-8. (PMID: 33409834)
J Bone Miner Res. 2010 Jul;25(7):1604-15. (PMID: 20200974)
3D Print Med. 2020 Oct 2;6(1):29. (PMID: 33006702)
Diabetes Care. 2009 Jun;32(6):1119-31. (PMID: 19429873)
Int J Mol Sci. 2019 Sep 30;20(19):. (PMID: 31575077)
Am J Pathol. 2009 Oct;175(4):1574-85. (PMID: 19745063)
Curr Opin Endocrinol Diabetes Obes. 2009 Dec;16(6):435-45. (PMID: 19779334)
Comput Assist Surg (Abingdon). 2021 Dec;26(1):15-21. (PMID: 33625935)
J Orthop Res. 2013 May;31(5):792-9. (PMID: 23255164)
J Diabetes Res. 2021 Jun 23;2021:6707464. (PMID: 34258293)
Front Endocrinol (Lausanne). 2013 Mar 08;4:21. (PMID: 23482417)
Endocrinology. 2007 Jan;148(1):198-205. (PMID: 17053023)
Materials (Basel). 2014 Dec 19;7(12):8168-8188. (PMID: 28788296)
Injury. 2011 Jun;42(6):569-73. (PMID: 21489531)
Curr Diab Rep. 2017 Feb;17(2):13. (PMID: 28265893)
J Cell Biochem. 2000 Aug 2;79(2):301-10. (PMID: 10967557)
Front Immunol. 2020 Jul 22;11:1582. (PMID: 32793223)
Orthop Res Rev. 2016 Oct 14;8:57-66. (PMID: 30774470)
Diabetol Metab Syndr. 2017 Oct 19;9:85. (PMID: 29075333)
J Orthop Res. 2016 Mar;34(3):369-85. (PMID: 26488900)
Orthop Surg. 2022 Jun;14(6):1217-1228. (PMID: 35451209)
Foot Ankle Int. 2022 Jun;43(6):750-761. (PMID: 35209733)
J Diabetes Complications. 2010 Sep-Oct;24(5):334-44. (PMID: 19628413)
Acta Biomater. 2016 Jan;30:345-356. (PMID: 26523335)
J Spine Surg. 2022 Sep;8(3):300-303. (PMID: 36285096)
Implant Dent. 2015 Feb;24(1):83-91. (PMID: 25621554)
Osteoporos Int. 2006;17(7):986-95. (PMID: 16552468)
Popul Health Manag. 2017 Feb;20(1):6-12. (PMID: 27124621)
Bone. 2005 Oct;37(4):482-90. (PMID: 16027060)
Bone. 2012 Jan;50(1):276-88. (PMID: 22086137)
Diabetol Int. 2020 Aug 30;12(2):171-180. (PMID: 33786272)
J Biomed Mater Res A. 2021 Oct;109(10):1792-1802. (PMID: 33754494)
Int J Mol Sci. 2021 Apr 16;22(8):. (PMID: 33923498)
Clin Chim Acta. 2006 Sep;371(1-2):32-6. (PMID: 16777084)
ACS Omega. 2020 Oct 07;5(41):26655-26666. (PMID: 33110992)
Bonekey Rep. 2014 Nov 12;3:585. (PMID: 25396049)
J Am Acad Orthop Surg Glob Res Rev. 2021 Apr 20;5(4):e20.00230-11. (PMID: 33877073)
Diabetes Metab Res Rev. 2010 Nov;26(8):622-30. (PMID: 20938995)
Heliyon. 2021 May 07;7(5):e06892. (PMID: 34027149)
PLoS One. 2022 Jun 17;17(6):e0270001. (PMID: 35714142)
Am J Physiol Endocrinol Metab. 2011 Dec;301(6):E1220-8. (PMID: 21900121)
Med Oral Patol Oral Cir Bucal. 2019 Jul 1;24(4):e425-e432. (PMID: 31246936)
فهرسة مساهمة: Keywords: 3D printing; BGLAP; Bmp7; Charcot; additive manufacturing; diabetes; implants; osteointegration
المشرفين على المادة: D1JT611TNE (Titanium)
IY9XDZ35W2 (Glucose)
0 (Bone Morphogenetic Protein 7)
0 (Collagen Type I)
0 (Collagen Type I, alpha 1 Chain)
تواريخ الأحداث: Date Created: 20240718 Date Completed: 20240718 Latest Revision: 20240719
رمز التحديث: 20240719
مُعرف محوري في PubMed: PMC11251957
DOI: 10.3389/fendo.2024.1346094
PMID: 39022341
قاعدة البيانات: MEDLINE
الوصف
تدمد:1664-2392
DOI:10.3389/fendo.2024.1346094