دورية أكاديمية
Diagnosis of Chagas Disease by Detecting Species-Specific Repetitive Genomic DNA from Filtered Human Urine Samples.
العنوان: | Diagnosis of Chagas Disease by Detecting Species-Specific Repetitive Genomic DNA from Filtered Human Urine Samples. |
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المؤلفون: | Price M; Marquette University College of Health Sciences, Milwaukee, Wisconsin., Lodh N; Marquette University College of Health Sciences, Milwaukee, Wisconsin, nilanjan.lodh@marquette.edu. |
المصدر: | WMJ : official publication of the State Medical Society of Wisconsin [WMJ] 2024 Jul; Vol. 123 (3), pp. 199-203. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: University of Wisconsin School of Medicine and Public Health Country of Publication: United States NLM ID: 9716054 Publication Model: Print Cited Medium: Internet ISSN: 2379-3961 (Electronic) Linking ISSN: 10981861 NLM ISO Abbreviation: WMJ Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 2019- : Madison, WI : Milwaukee, WI : University of Wisconsin School of Medicine and Public Health ; Medical College of Wisconsin Original Publication: Madison, WI : State Medical Society of Wisconsin, c1997- |
مواضيع طبية MeSH: | Chagas Disease*/diagnosis , Chagas Disease*/urine , Polymerase Chain Reaction* , DNA, Protozoan*/urine , Trypanosoma cruzi*/genetics , Trypanosoma cruzi*/isolation & purification, Humans ; Sensitivity and Specificity ; Argentina/epidemiology ; Species Specificity |
مستخلص: | Introduction: Chagas disease caused by Trypanosoma cruzi (T cruzi) found in the Americas is often missed during the early stage of infection due to lack of sensitive diagnostic tests. The classic immunological and parasitological tests often fail in the acute phase due to the nonspecific and low antibody level nature of the infection and in the chronic phase due to low levels of trypanosomes in the blood. For successful control strategies, there must be a sensitive and specific diagnostic test. Objective/methods: We have demonstrated the possibility (proof of concept) of detecting T cruzi -specific repeat DNA via polymerase chain reaction (PCR) by (1) spiking 15 urine samples collected from volunteers free of prior infection with 3 different concentrations of T cruzi (3 strains), Trypanosoma brucei , and Trypanosoma rhodesiense (African strain) genomic DNA and (2) from filtered collected clinical samples from Argentina. Three sets of primers were used. Results: Our approach detected repeat DNA specific for T cruzi strains from 1 clinical sample by 2 sets of primer and from spiked urine by all 3 sets of primer but not the African species. A serial dilution (spiking) also was performed on T cruzi strains to detect sensitivities of the assay. One set of primers constantly detected satellite DNA for all T cruzi strains from 70 pg/μl to 175 fg/μl. Conclusions: We were able to demonstrate the feasibility of detecting T cruzi -specific DNA from filtered urine samples by sensitive and specific PCR assay. Besides the evident increased sensitivity and specificity of primers, our approach can be used to explore Chagas prevalence in endemic areas - especially in congenital Chagas newborn screening - and in the acute phase. (Copyright© Board of Regents of the University of Wisconsin System and The Medical College of Wisconsin, Inc.) |
المشرفين على المادة: | 0 (DNA, Protozoan) |
تواريخ الأحداث: | Date Created: 20240718 Date Completed: 20240718 Latest Revision: 20240718 |
رمز التحديث: | 20240719 |
PMID: | 39024145 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2379-3961 |
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