دورية أكاديمية
Regulation role of miR-204 on SIRT1/VEGF in metabolic memory induced by high glucose in human retinal pigment epithelial cells.
| العنوان: | Regulation role of miR-204 on SIRT1/VEGF in metabolic memory induced by high glucose in human retinal pigment epithelial cells. |
|---|---|
| المؤلفون: | Lai QL; Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan 460060, Hubei Province, China., Xie T; Department of Ophthalmology, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, Fujian Province, China., Zheng WD; Department of Ophthalmology, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, Fujian Province, China., Huang Y; Department of Ophthalmology & Optometry, Fujian Medical University, Fuzhou 350004, Fujian Province, China. |
| المصدر: | International journal of ophthalmology [Int J Ophthalmol] 2024 Jul 18; Vol. 17 (7), pp. 1232-1237. Date of Electronic Publication: 2024 Jul 18 (Print Publication: 2024). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Press of International Journal of Ophthalmology Country of Publication: China NLM ID: 101553860 Publication Model: eCollection Cited Medium: Print ISSN: 2222-3959 (Print) Linking ISSN: 22223959 NLM ISO Abbreviation: Int J Ophthalmol Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Xi'an, China : Press of International Journal of Ophthalmology |
| مستخلص: | Aim: To examine the regulatory role of microRNA-204 (miR-204) on silent information regulator 1 (SIRT1) and vascular endothelial growth factor (VEGF) under high-glucose-induced metabolic memory in human retinal pigment epithelial (hRPE) cells. Methods: Cells were cultured with either normal (5 mmol/L) or high D-glucose (25 mmol/L) concentrations for 8d to establish control and high-glucose groups, respectively. To induce metabolic memory, cells were cultured with 25 mmol/L D-glucose for 4d followed by culture with 5 mmol/L D-glucose for 4d. In addition, exposed in 25 mmol/L D-glucose for 4d and then transfected with 100 nmol/L miR-204 control, miR-204 inhibitor or miR-204 mimic in 5 mmol/L D-glucose for 4d. Quantitative reverse transcription-polymerase chain reaction (RT-qPCR) was used to detect miR-204 mRNA levels. SIRT1 and VEGF protein levels were assessed by immunohistochemical and Western blot. Flow cytometry was used to investigate apoptosis rate. Results: It was found that high glucose promoted miR-204 and VEGF expression, and inhibited SIRT1 activity, even after the return to normal glucose culture conditions. Upregulation of miR-204 promoted apoptosis inhibiting SIRT1 and increasing VEGF expression. However, downregulation of miR-204 produced the opposite effects. Conclusion: The study identifies that miR-204 is the upstream target of SIRT1 and VEGF, and that miR-204 can protect hRPE cells from the damage caused by metabolic memory through increasing SIRT1 and inhibiting VEGF expression. Competing Interests: Conflicts of Interest: Lai QL, None; Xie T, None; Zheng WD, None; Huang Y, None. (International Journal of Ophthalmology Press.) |
| References: | J Biochem Mol Toxicol. 2023 Aug;37(8):e23398. (PMID: 37421224) Diabetes Care. 2004 May;27(5):1047-53. (PMID: 15111519) Biomarkers. 2020 Jul;25(5):397-401. (PMID: 32529845) Mayo Clin Proc. 2021 Feb;96(2):322-331. (PMID: 33549254) Int J Mol Sci. 2023 Apr 29;24(9):. (PMID: 37175784) Cells. 2022 Oct 25;11(21):. (PMID: 36359761) Transl Vis Sci Technol. 2020 May 19;9(6):16. (PMID: 32821513) Saudi J Biol Sci. 2021 Jan;28(1):775-784. (PMID: 33424367) Diabetologia. 2016 Mar;59(3):644-54. (PMID: 26687158) Int J Ophthalmol. 2023 Jan 18;16(1):88-94. (PMID: 36659946) Int J Ophthalmol. 2023 Nov 18;16(11):1766-1772. (PMID: 38028519) Front Physiol. 2020 Jan 29;10:1621. (PMID: 32063865) Biosci Rep. 2020 May 29;40(5):. (PMID: 32347300) Biosci Rep. 2020 Jan 31;40(1):. (PMID: 31894851) J Pers Med. 2023 Dec 22;14(1):. (PMID: 38248719) Oxid Med Cell Longev. 2021 Oct 23;2021:1641717. (PMID: 34725563) Arterioscler Thromb Vasc Biol. 2016 May;36(5):864-73. (PMID: 26941017) Front Endocrinol (Lausanne). 2023 Aug 25;14:1196335. (PMID: 37693349) JAMA. 2020 Dec 15;324(23):2383-2395. (PMID: 33320223) Genes (Basel). 2022 Apr 15;13(4):. (PMID: 35456511) Clin Epigenetics. 2024 Jan 3;16(1):6. (PMID: 38172938) Medicina (Kaunas). 2023 May 07;59(5):. (PMID: 37241128) Eur Rev Med Pharmacol Sci. 2020 Jun;24(12):6486-6493. (PMID: 32633335) J Recept Signal Transduct Res. 2023 Dec;43(2):62-71. (PMID: 37330920) Life (Basel). 2023 Apr 27;13(5):. (PMID: 37240743) Pharmacology. 2021;106(9-10):520-533. (PMID: 34352784) Arch Soc Esp Oftalmol (Engl Ed). 2024 Feb;99(2):62-66. (PMID: 38013130) Inflamm Res. 2021 Apr;70(4):445-457. (PMID: 33609142) Indian J Ophthalmol. 2023 Aug;71(8):3080-3084. (PMID: 37530284) Curr Diabetes Rev. 2024 Jan 25;:. (PMID: 38369731) Int J Ophthalmol. 2023 Sep 18;16(9):1456-1464. (PMID: 37724274) Invest Ophthalmol Vis Sci. 2017 Dec 1;58(14):6241-6247. (PMID: 29228252) Peptides. 2023 Oct;168:171065. (PMID: 37495040) |
| فهرسة مساهمة: | Keywords: high-glucose; human retinal pigment epithelial; metabolic memory; microRNA-204; silent information regulator 1; vascular endothelial growth factor |
| تواريخ الأحداث: | Date Created: 20240719 Latest Revision: 20240720 |
| رمز التحديث: | 20240720 |
| مُعرف محوري في PubMed: | PMC11246945 |
| DOI: | 10.18240/ijo.2024.07.06 |
| PMID: | 39026923 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2222-3959 |
|---|---|
| DOI: | 10.18240/ijo.2024.07.06 |