دورية أكاديمية
Regulation role of miR-204 on SIRT1/VEGF in metabolic memory induced by high glucose in human retinal pigment epithelial cells.
العنوان: | Regulation role of miR-204 on SIRT1/VEGF in metabolic memory induced by high glucose in human retinal pigment epithelial cells. |
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المؤلفون: | Lai QL; Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan 460060, Hubei Province, China., Xie T; Department of Ophthalmology, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, Fujian Province, China., Zheng WD; Department of Ophthalmology, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, Fujian Province, China., Huang Y; Department of Ophthalmology & Optometry, Fujian Medical University, Fuzhou 350004, Fujian Province, China. |
المصدر: | International journal of ophthalmology [Int J Ophthalmol] 2024 Jul 18; Vol. 17 (7), pp. 1232-1237. Date of Electronic Publication: 2024 Jul 18 (Print Publication: 2024). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Press of International Journal of Ophthalmology Country of Publication: China NLM ID: 101553860 Publication Model: eCollection Cited Medium: Print ISSN: 2222-3959 (Print) Linking ISSN: 22223959 NLM ISO Abbreviation: Int J Ophthalmol Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Xi'an, China : Press of International Journal of Ophthalmology |
مستخلص: | Aim: To examine the regulatory role of microRNA-204 (miR-204) on silent information regulator 1 (SIRT1) and vascular endothelial growth factor (VEGF) under high-glucose-induced metabolic memory in human retinal pigment epithelial (hRPE) cells. Methods: Cells were cultured with either normal (5 mmol/L) or high D-glucose (25 mmol/L) concentrations for 8d to establish control and high-glucose groups, respectively. To induce metabolic memory, cells were cultured with 25 mmol/L D-glucose for 4d followed by culture with 5 mmol/L D-glucose for 4d. In addition, exposed in 25 mmol/L D-glucose for 4d and then transfected with 100 nmol/L miR-204 control, miR-204 inhibitor or miR-204 mimic in 5 mmol/L D-glucose for 4d. Quantitative reverse transcription-polymerase chain reaction (RT-qPCR) was used to detect miR-204 mRNA levels. SIRT1 and VEGF protein levels were assessed by immunohistochemical and Western blot. Flow cytometry was used to investigate apoptosis rate. Results: It was found that high glucose promoted miR-204 and VEGF expression, and inhibited SIRT1 activity, even after the return to normal glucose culture conditions. Upregulation of miR-204 promoted apoptosis inhibiting SIRT1 and increasing VEGF expression. However, downregulation of miR-204 produced the opposite effects. Conclusion: The study identifies that miR-204 is the upstream target of SIRT1 and VEGF, and that miR-204 can protect hRPE cells from the damage caused by metabolic memory through increasing SIRT1 and inhibiting VEGF expression. Competing Interests: Conflicts of Interest: Lai QL, None; Xie T, None; Zheng WD, None; Huang Y, None. (International Journal of Ophthalmology Press.) |
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فهرسة مساهمة: | Keywords: high-glucose; human retinal pigment epithelial; metabolic memory; microRNA-204; silent information regulator 1; vascular endothelial growth factor |
تواريخ الأحداث: | Date Created: 20240719 Latest Revision: 20240720 |
رمز التحديث: | 20240720 |
مُعرف محوري في PubMed: | PMC11246945 |
DOI: | 10.18240/ijo.2024.07.06 |
PMID: | 39026923 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2222-3959 |
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DOI: | 10.18240/ijo.2024.07.06 |