دورية أكاديمية
Validation of a rapid collagenase activity detection technique based on fluorescent quenched gelatin with synovial fluid samples.
العنوان: | Validation of a rapid collagenase activity detection technique based on fluorescent quenched gelatin with synovial fluid samples. |
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المؤلفون: | de la Fuente M; Department of Cell Biology and Histology, Experimental Ophthalmo-Biology Group (GOBE, www.ehu.eus/gobe), University of the Basque Country UPV/EHU, Leioa, 48940, Spain., Delgado D; Advanced Biological Therapy Unit, Hospital Vithas Vitoria, Vitoria-Gasteiz, 01008, Spain., Beitia M; Advanced Biological Therapy Unit, Hospital Vithas Vitoria, Vitoria-Gasteiz, 01008, Spain., Barreda-Gómez G; Research and Development Division, IMG Pharma Biotech, Zamudio, Bizkaia, Spain., Acera A; Department of Cell Biology and Histology, Experimental Ophthalmo-Biology Group (GOBE, www.ehu.eus/gobe), University of the Basque Country UPV/EHU, Leioa, 48940, Spain.; Ikerbasque, Basque Foundation for Science, Bilbao, 48001, Spain., Sanchez M; Advanced Biological Therapy Unit, Hospital Vithas Vitoria, Vitoria-Gasteiz, 01008, Spain.; Arthroscopic Surgery Unit, Hospital Vithas Vitoria, Vitoria-Gasteiz, 01008, Spain., Vecino E; Department of Cell Biology and Histology, Experimental Ophthalmo-Biology Group (GOBE, www.ehu.eus/gobe), University of the Basque Country UPV/EHU, Leioa, 48940, Spain. elena.vecino@ehu.eus. |
المصدر: | BMC biotechnology [BMC Biotechnol] 2024 Jul 19; Vol. 24 (1), pp. 50. Date of Electronic Publication: 2024 Jul 19. |
نوع المنشور: | Journal Article; Validation Study |
اللغة: | English |
بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101088663 Publication Model: Electronic Cited Medium: Internet ISSN: 1472-6750 (Electronic) Linking ISSN: 14726750 NLM ISO Abbreviation: BMC Biotechnol Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: [London] : BioMed Central, 2001- |
مواضيع طبية MeSH: | Synovial Fluid*/chemistry , Synovial Fluid*/enzymology , Synovial Fluid*/metabolism , Gelatin*/chemistry , Matrix Metalloproteinase 9*/metabolism, Humans ; Collagenases/metabolism ; Fluorescent Dyes/chemistry |
مستخلص: | Background: Measuring collagenase activity is crucial in the field of joint health and disease management. Collagenases, enzymes responsible for collagen degradation, play a vital role in maintaining the balance between collagen synthesis and breakdown in joints. Dysregulation of collagenase activity leads to joint tissue degradation and diseases such as rheumatoid arthritis and osteoarthritis. The development of methods to measure collagenase activity is essential for diagnosis, disease severity assessment, treatment monitoring, and identification of therapeutic targets. Results: This study aimed to validate a rapid collagenase activity detection technique using synovial fluid samples. Antibody microarray analysis was initially performed to quantify the levels of matrix metalloproteinase-9 (MMP-9), a major collagenase in joints. Subsequently, the developed gelatin-based test utilizing fluorescence measurement was used to determine collagenase activity. There was a significant correlation between the presence of MMP-9 and collagenase activity. In addition, Lower Limit of Detection and Upper Limit of Detection can be preliminary estimated as 8 ng/mL and 48 ng/mL respectively. Conclusions: The developed technique offers a potential point-of-care assessment of collagenase activity, providing real-time information for clinicians and researchers. By accurately quantifying collagenase activity, healthcare professionals can optimize patient care, improve treatment outcomes, and contribute to the understanding and management of joint-related disorders. Further research and validation are necessary to establish the full potential of this rapid collagenase activity detection method in clinical practice. (© 2024. The Author(s).) |
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معلومات مُعتمدة: | 48-AF-W2-2019-00006 Basque Government (BIKAINTEK); 201900016247 University of the Basque Country (PIFIND19/02); FISS-21-RD21/0002/0041 FISS; KK-2019/00086 ELKARTEK; PID2019-111139RB-I00 MINECO-Retos |
فهرسة مساهمة: | Keywords: Biomarker; Collagenase activity; Gelatin; In vitro diagnosis; MMP-9; Point of care |
المشرفين على المادة: | 9000-70-8 (Gelatin) EC 3.4.24.35 (Matrix Metalloproteinase 9) EC 3.4.24.- (Collagenases) 0 (Fluorescent Dyes) EC 3.4.24.35 (MMP9 protein, human) |
تواريخ الأحداث: | Date Created: 20240719 Date Completed: 20240719 Latest Revision: 20240725 |
رمز التحديث: | 20240726 |
مُعرف محوري في PubMed: | PMC11264812 |
DOI: | 10.1186/s12896-024-00869-y |
PMID: | 39030513 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1472-6750 |
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DOI: | 10.1186/s12896-024-00869-y |