دورية أكاديمية
أعرض في EDS

Validation of a rapid collagenase activity detection technique based on fluorescent quenched gelatin with synovial fluid samples.

التفاصيل البيبلوغرافية
العنوان: Validation of a rapid collagenase activity detection technique based on fluorescent quenched gelatin with synovial fluid samples.
المؤلفون: de la Fuente M; Department of Cell Biology and Histology, Experimental Ophthalmo-Biology Group (GOBE, www.ehu.eus/gobe), University of the Basque Country UPV/EHU, Leioa, 48940, Spain., Delgado D; Advanced Biological Therapy Unit, Hospital Vithas Vitoria, Vitoria-Gasteiz, 01008, Spain., Beitia M; Advanced Biological Therapy Unit, Hospital Vithas Vitoria, Vitoria-Gasteiz, 01008, Spain., Barreda-Gómez G; Research and Development Division, IMG Pharma Biotech, Zamudio, Bizkaia, Spain., Acera A; Department of Cell Biology and Histology, Experimental Ophthalmo-Biology Group (GOBE, www.ehu.eus/gobe), University of the Basque Country UPV/EHU, Leioa, 48940, Spain.; Ikerbasque, Basque Foundation for Science, Bilbao, 48001, Spain., Sanchez M; Advanced Biological Therapy Unit, Hospital Vithas Vitoria, Vitoria-Gasteiz, 01008, Spain.; Arthroscopic Surgery Unit, Hospital Vithas Vitoria, Vitoria-Gasteiz, 01008, Spain., Vecino E; Department of Cell Biology and Histology, Experimental Ophthalmo-Biology Group (GOBE, www.ehu.eus/gobe), University of the Basque Country UPV/EHU, Leioa, 48940, Spain. elena.vecino@ehu.eus.
المصدر: BMC biotechnology [BMC Biotechnol] 2024 Jul 19; Vol. 24 (1), pp. 50. Date of Electronic Publication: 2024 Jul 19.
نوع المنشور: Journal Article; Validation Study
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101088663 Publication Model: Electronic Cited Medium: Internet ISSN: 1472-6750 (Electronic) Linking ISSN: 14726750 NLM ISO Abbreviation: BMC Biotechnol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : BioMed Central, 2001-
مواضيع طبية MeSH: Synovial Fluid*/chemistry , Synovial Fluid*/enzymology , Synovial Fluid*/metabolism , Gelatin*/chemistry , Matrix Metalloproteinase 9*/metabolism, Humans ; Collagenases/metabolism ; Fluorescent Dyes/chemistry
مستخلص: Background: Measuring collagenase activity is crucial in the field of joint health and disease management. Collagenases, enzymes responsible for collagen degradation, play a vital role in maintaining the balance between collagen synthesis and breakdown in joints. Dysregulation of collagenase activity leads to joint tissue degradation and diseases such as rheumatoid arthritis and osteoarthritis. The development of methods to measure collagenase activity is essential for diagnosis, disease severity assessment, treatment monitoring, and identification of therapeutic targets.
Results: This study aimed to validate a rapid collagenase activity detection technique using synovial fluid samples. Antibody microarray analysis was initially performed to quantify the levels of matrix metalloproteinase-9 (MMP-9), a major collagenase in joints. Subsequently, the developed gelatin-based test utilizing fluorescence measurement was used to determine collagenase activity. There was a significant correlation between the presence of MMP-9 and collagenase activity. In addition, Lower Limit of Detection and Upper Limit of Detection can be preliminary estimated as 8 ng/mL and 48 ng/mL respectively.
Conclusions: The developed technique offers a potential point-of-care assessment of collagenase activity, providing real-time information for clinicians and researchers. By accurately quantifying collagenase activity, healthcare professionals can optimize patient care, improve treatment outcomes, and contribute to the understanding and management of joint-related disorders. Further research and validation are necessary to establish the full potential of this rapid collagenase activity detection method in clinical practice.
(© 2024. The Author(s).)
References: Coll Relat Res. 1983 May;3(3):205-15. (PMID: 6191911)
Rheumatol Int. 2011 Apr;31(4):543-7. (PMID: 20665024)
Am J Pathol. 2009 Jan;174(1):101-14. (PMID: 19116368)
Arthritis Rheum. 1996 Sep;39(9):1576-87. (PMID: 8814070)
Arthritis Res Ther. 2012 Jun 07;14(3):212. (PMID: 22682469)
Radiol Med. 2019 Nov;124(11):1128-1141. (PMID: 30880357)
Biomater Biosyst. 2024 Feb 21;13:100090. (PMID: 38440290)
Carcinogenesis. 1999 May;20(5):749-55. (PMID: 10334190)
J Biol Chem. 1997 Jan 24;272(4):2446-51. (PMID: 8999957)
Eur Respir J. 2011 Jul;38(1):191-208. (PMID: 21177845)
World J Biol Chem. 2011 Jan 26;2(1):14-24. (PMID: 21537473)
Arthritis Rheum. 1983 Jan;26(1):63-8. (PMID: 6297508)
Mol Med Rep. 2017 Aug;16(2):1837-1845. (PMID: 28627596)
J Rheumatol. 1994 Sep;21(9):1593-6. (PMID: 7799334)
Arthritis Res Ther. 2006;8(6):R187. (PMID: 17177994)
Mol Vis. 2021 May 08;27:243-261. (PMID: 34012227)
Clin Chem Lab Med. 2016 Aug 1;54(8):1281-90. (PMID: 26641968)
J Biol Chem. 1998 Sep 11;273(37):23959-68. (PMID: 9727011)
Sci Rep. 2019 Sep 17;9(1):13432. (PMID: 31530842)
FEBS Lett. 2002 Apr 24;517(1-3):206-10. (PMID: 12062438)
Scand J Rheumatol. 1986;15(3):237-42. (PMID: 3026034)
Calcif Tissue Int. 2021 Sep;109(3):277-290. (PMID: 32772139)
J Clin Chem Clin Biochem. 1989 Jun;27(6):351-9. (PMID: 2547014)
Expert Opin Ther Targets. 2022 Feb;26(2):93-105. (PMID: 35081858)
Ophthalmic Res. 2008 Oct;40(6):315-21. (PMID: 18688174)
Cornea. 2011 Jan;30(1):42-9. (PMID: 20861728)
Arch Oral Biol. 2016 Aug;68:66-72. (PMID: 27105041)
Methods Mol Biol. 2017;1626:79-102. (PMID: 28608202)
J Infect Dis. 2014 Apr 1;209(7):1105-15. (PMID: 24277745)
J Biochem. 1973 May;73(5):1007-11. (PMID: 4124619)
J Bone Joint Surg Am. 2014 Aug 6;96(15):e130. (PMID: 25100783)
Osteoarthritis Cartilage. 2004 May;12(5):409-18. (PMID: 15094140)
Ital J Biochem. 2005 Sep-Dec;54(3-4):248-57. (PMID: 16688934)
Sci Rep. 2017 Mar 13;7:44321. (PMID: 28287171)
Medicina (Kaunas). 2023 Jan 14;59(1):. (PMID: 36676791)
Methods Mol Biol. 2001;151:495-518. (PMID: 11217324)
Anal Biochem. 2014 May 15;453:61-9. (PMID: 24608089)
Ann Rheum Dis. 2005 May;64(5):694-8. (PMID: 15834054)
Int J Mol Sci. 2022 May 18;23(10):. (PMID: 35628448)
J Immunol Res. 2015;2015:192415. (PMID: 26064995)
J Dev Biol. 2015 Dec;3(4):177-192. (PMID: 27347486)
Int J Mol Sci. 2021 Feb 09;22(4):. (PMID: 33572320)
Arthritis Res Ther. 2007;9(5):220. (PMID: 18001488)
Pediatr Res. 2001 Jul;50(1):29-33. (PMID: 11420415)
Clin Chim Acta. 2003 Mar;329(1-2):77-81. (PMID: 12589968)
Int J Biochem Cell Biol. 2004 Mar;36(3):372-8. (PMID: 14687914)
Osteoarthritis Cartilage. 2009 Jan;17(1):100-6. (PMID: 18595745)
Ann Rheum Dis. 1996 Feb;55(2):147. (PMID: 8712868)
Arthritis Rheum. 1969 Apr;12(2):92-102. (PMID: 4305063)
Magn Reson Imaging. 2020 Jan;65:27-36. (PMID: 31670237)
J Dent Res. 1995 May;74(5):1226-34. (PMID: 7790601)
Methods Mol Biol. 2017;1579:231-244. (PMID: 28299740)
Anal Biochem. 1987 Oct;166(1):172-7. (PMID: 2823639)
Ann Rheum Dis. 1993 Dec;52(12):866-9. (PMID: 8311537)
Int Immunopharmacol. 2014 Nov;23(1):211-21. (PMID: 25203593)
Arthritis Rheum. 1996 Sep;39(9):1455-65. (PMID: 8814056)
Osteoarthr Cartil Open. 2022 Jan 21;4(1):100235. (PMID: 36474471)
Clin Orthop Relat Res. 1975 Jul-Aug;(110):303-16. (PMID: 168997)
Arthritis Rheum. 1993 Mar;36(3):372-9. (PMID: 8452582)
Invest Ophthalmol Vis Sci. 2013 Dec 23;54(13):8285-91. (PMID: 24255042)
Front Immunol. 2020 Mar 10;11:195. (PMID: 32210952)
Ann Rheum Dis. 2004 Jul;63(7):881-3. (PMID: 15194590)
Arthritis Res. 2002;4(3):157-64. (PMID: 12010565)
Anal Bioanal Chem. 2021 Apr;413(10):2709-2719. (PMID: 33206215)
Ann Rheum Dis. 1995 Dec;54(12):970-5. (PMID: 8546529)
Am J Physiol Cell Physiol. 2001 Jan;280(1):C53-60. (PMID: 11121376)
J Ultrason. 2017 Mar;17(68):5-16. (PMID: 28439423)
Mol Hum Reprod. 2006 Oct;12(10):633-41. (PMID: 16935996)
Curr Issues Mol Biol. 2023 Apr 01;45(4):2881-2894. (PMID: 37185712)
Cartilage. 2021 Dec;13(2_suppl):1421S-1430S. (PMID: 32748631)
J Mol Biol. 2013 May 27;425(10):1815-25. (PMID: 23399546)
معلومات مُعتمدة: 48-AF-W2-2019-00006 Basque Government (BIKAINTEK); 201900016247 University of the Basque Country (PIFIND19/02); FISS-21-RD21/0002/0041 FISS; KK-2019/00086 ELKARTEK; PID2019-111139RB-I00 MINECO-Retos
فهرسة مساهمة: Keywords: Biomarker; Collagenase activity; Gelatin; In vitro diagnosis; MMP-9; Point of care
المشرفين على المادة: 9000-70-8 (Gelatin)
EC 3.4.24.35 (Matrix Metalloproteinase 9)
EC 3.4.24.- (Collagenases)
0 (Fluorescent Dyes)
EC 3.4.24.35 (MMP9 protein, human)
تواريخ الأحداث: Date Created: 20240719 Date Completed: 20240719 Latest Revision: 20240725
رمز التحديث: 20240726
مُعرف محوري في PubMed: PMC11264812
DOI: 10.1186/s12896-024-00869-y
PMID: 39030513
قاعدة البيانات: MEDLINE
الوصف
تدمد:1472-6750
DOI:10.1186/s12896-024-00869-y