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Loss of bmp15 function in the seasonal spawner Atlantic salmon results in ovulatory failure.

التفاصيل البيبلوغرافية
العنوان: Loss of bmp15 function in the seasonal spawner Atlantic salmon results in ovulatory failure.
المؤلفون: Crespo D; Research Group Reproduction and Developmental Biology, Institute of Marine Research, Bergen, Norway., Fjelldal PG; Research Group Reproduction and Developmental Biology, Institute of Marine Research, Matre Research Station, Matredal, Norway., Hansen TJ; Research Group Reproduction and Developmental Biology, Institute of Marine Research, Matre Research Station, Matredal, Norway., Kjærner-Semb E; Research Group Reproduction and Developmental Biology, Institute of Marine Research, Bergen, Norway., Skaftnesmo KO; Research Group Reproduction and Developmental Biology, Institute of Marine Research, Bergen, Norway., Thorsen A; Research Group Reproduction and Developmental Biology, Institute of Marine Research, Bergen, Norway., Norberg B; Research Group Reproduction and Developmental Biology, Institute of Marine Research, Austevoll Research Station, Haukanes, Norway., Edvardsen RB; Research Group Reproduction and Developmental Biology, Institute of Marine Research, Bergen, Norway., Andersson E; Research Group Reproduction and Developmental Biology, Institute of Marine Research, Bergen, Norway., Schulz RW; Research Group Reproduction and Developmental Biology, Institute of Marine Research, Bergen, Norway.; Reproductive Biology Group, Division Developmental Biology, Department Biology, Science Faculty, Utrecht University, Utrecht, The Netherlands., Wargelius A; Research Group Reproduction and Developmental Biology, Institute of Marine Research, Bergen, Norway., Kleppe L; Research Group Reproduction and Developmental Biology, Institute of Marine Research, Bergen, Norway.
المصدر: FASEB journal : official publication of the Federation of American Societies for Experimental Biology [FASEB J] 2024 Jul 31; Vol. 38 (14), pp. e23837.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Federation of American Societies for Experimental Biology Country of Publication: United States NLM ID: 8804484 Publication Model: Print Cited Medium: Internet ISSN: 1530-6860 (Electronic) Linking ISSN: 08926638 NLM ISO Abbreviation: FASEB J Subsets: MEDLINE
أسماء مطبوعة: Publication: 2020- : [Bethesda, Md.] : Hoboken, NJ : Federation of American Societies for Experimental Biology ; Wiley
Original Publication: [Bethesda, Md.] : The Federation, [c1987-
مواضيع طبية MeSH: Bone Morphogenetic Protein 15*/genetics , Bone Morphogenetic Protein 15*/metabolism , Salmo salar*/metabolism , Salmo salar*/genetics , Salmo salar*/growth & development , Ovulation*, Animals ; Female ; Ovary/metabolism ; Ovarian Follicle/metabolism ; Oocytes/metabolism ; Male ; Fish Proteins/genetics ; Fish Proteins/metabolism ; Seasons
مستخلص: Bone morphogenetic protein 15 (BMP15) is an oocyte-specific growth factor important for successful female reproduction in mammals. While mutations in BMP15/Bmp15 cause ovulatory deficiency and/or infertility in certain mammalian species, loss of bmp15 in zebrafish, a continuous spawner and the only bmp15 knockout model in fish to date, results in complete arrest of follicle development and later female-to-male sex reversal, preventing to examine effects on ovulation/fertilization. Here, we used Atlantic salmon, a seasonal spawner, and generated bmp15 mutants to investigate ovarian development and fertility. Histological and morphometric analyses revealed that in biallelic frameshift (bmp15 fs/fs) mutant ovaries, folliculogenesis started earlier, resulting in an advanced development compared to wild-type (WT) controls, accompanied by a weaker expression of the (early) oocyte-specific factor figla. This precocious ovarian development was followed in bmp15 fs/fs females by enhanced follicle atresia during vitellogenic stages. Although genes involved in steroid synthesis and signaling (star, cyp11b, cyp17a1 and esr1) were dramatically higher in late vitellogenic bmp15 fs/fs mutant ovaries, estradiol-17β plasma levels were lower than in WT counterparts, potentially reflecting compensatory changes at the level of ovarian gene expression. At spawning, bmp15 fs/fs females displayed lower gonado-somatic index values and reduced oocyte diameter, and the majority (71.4%), showed mature non-ovulating ovaries with a high degree of atresia. The remaining (28.6%) females spawned eggs but they either could not be fertilized or, upon fertilization, showed severe malformations and embryonic mortality. Our results show that Bmp15 is required for proper follicle recruitment and growth and later ovulatory success in Atlantic salmon, providing an alternative candidate target to induce sterility in farmed salmon. Moreover, since loss of bmp15 in salmon, in contrast to zebrafish, does not result in female-to-male sex change, this is the first mutant model in fish allowing further investigations on Bmp15-mediated functions in the ovulatory period.
(© 2024 The Author(s). The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.)
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معلومات مُعتمدة: 267610 Norges Forskningsråd (Forskningsrådet); 245979 Norges Forskningsråd (Forskningsrådet); 302532 Norges Forskningsråd (Forskningsrådet)
فهرسة مساهمة: Keywords: Atlantic salmon; CRISPR/Cas9; bone morphogenetic protein 15; oogenesis; ovulation
المشرفين على المادة: 0 (Bone Morphogenetic Protein 15)
0 (Fish Proteins)
تواريخ الأحداث: Date Created: 20240720 Date Completed: 20240720 Latest Revision: 20240820
رمز التحديث: 20240820
DOI: 10.1096/fj.202400370R
PMID: 39031536
قاعدة البيانات: MEDLINE
الوصف
تدمد:1530-6860
DOI:10.1096/fj.202400370R