دورية أكاديمية
Encapsulation of Citrus sinensis essential oil and R-limonene in lipid nanocarriers: A potential strategy for the treatment of leishmaniasis.
| العنوان: | Encapsulation of Citrus sinensis essential oil and R-limonene in lipid nanocarriers: A potential strategy for the treatment of leishmaniasis. |
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| المؤلفون: | Santos JS; Departament of Pharmacy, Federal University of Sergipe, São Cristóvão 49100-000, Sergipe, Brazil. Electronic address: jeferson_sts@academico.ufs.br., Galvão JG; Departament of Pharmacy, Federal University of Sergipe, São Cristóvão 49100-000, Sergipe, Brazil., Mendonça MRC; Departament of Pharmacy, Federal University of Sergipe, São Cristóvão 49100-000, Sergipe, Brazil., Costa AMB; Departament of Pharmacy, Federal University of Sergipe, São Cristóvão 49100-000, Sergipe, Brazil., Silva ARST; Departament of Pharmacy, Federal University of Sergipe, São Cristóvão 49100-000, Sergipe, Brazil., Oliveira DS; Departament of Pharmacy, Federal University of Sergipe, São Cristóvão 49100-000, Sergipe, Brazil., Santos AJ; Process Engineering Program, University of Tiradentes (UNIT), Aracaju, SE 49032-490, Brazil., Lira AAM; Departament of Pharmacy, Federal University of Sergipe, São Cristóvão 49100-000, Sergipe, Brazil., Scher R; Departament of Morphology, Federal University of Sergipe, São Cristóovão 49100-000, Sergipe, Brazil., Sales Júnior PA; Aggeu Magalhães Institute (IAM), Oswaldo Cruz Foundation (FIOCRUZ), 50670-420 Recife, PE, Brazil., Pereira VRA; Aggeu Magalhães Institute (IAM), Oswaldo Cruz Foundation (FIOCRUZ), 50670-420 Recife, PE, Brazil., Formiga FR; Aggeu Magalhães Institute (IAM), Oswaldo Cruz Foundation (FIOCRUZ), 50670-420 Recife, PE, Brazil; Faculty of Medical Sciences (FCM), University of Pernambuco (UPE), 52171-011 Recife, PE, Brazil., Nunes RS; Departament of Pharmacy, Federal University of Sergipe, São Cristóvão 49100-000, Sergipe, Brazil. |
| المصدر: | International journal of pharmaceutics [Int J Pharm] 2024 Sep 05; Vol. 662, pp. 124464. Date of Electronic Publication: 2024 Jul 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 7804127 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3476 (Electronic) Linking ISSN: 03785173 NLM ISO Abbreviation: Int J Pharm Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press. |
| مواضيع طبية MeSH: | Oils, Volatile*/administration & dosage , Oils, Volatile*/chemistry , Oils, Volatile*/pharmacology , Limonene*/chemistry , Limonene*/administration & dosage , Limonene*/pharmacology , Drug Carriers*/chemistry , Cell Survival*/drug effects , Chitosan*/chemistry , Chitosan*/administration & dosage , Lipids*/chemistry , Lipids*/administration & dosage , Nanoparticles*/chemistry , Nanoparticles*/administration & dosage , Citrus sinensis*/chemistry, Animals ; Mice ; RAW 264.7 Cells ; Antiprotozoal Agents/administration & dosage ; Antiprotozoal Agents/pharmacology ; Antiprotozoal Agents/chemistry ; Leishmaniasis/drug therapy ; Particle Size ; Cell Line ; Leishmania/drug effects ; Terpenes/chemistry ; Terpenes/pharmacology ; Terpenes/administration & dosage ; Nanostructures/chemistry ; Nanostructures/administration & dosage |
| مستخلص: | Leishmaniases, a group of neglected tropical diseases caused by an intracellular parasite of the genus Leishmania, have significant impacts on global health. Current treatment options are limited due to drug resistance, toxicity, and high cost. This study aimed to develop nanostructured lipid carriers (NLCs) for delivering Citrus sinensis essential oil (CSEO) and its main constituent, R-limonene, against leishmaniasis. The influence of surface-modified NLCs using chitosan was also examined. The NLCs were prepared using a warm microemulsion method, and surface modification with chitosan was achieved through electrostatic interaction. These nanocarriers were characterized by differential scanning calorimetry (DSC), X-ray diffraction (XRD), transmission electron microscopy, and dynamic light scattering (DLS). In vitro cytotoxicity was assessed in L929 and RAW 264.7 cells, and leishmanicidal activity was evaluated against promastigote and amastigote forms. The NLCs were spherical, with particle sizes ranging from 97.9 nm to 111.3 nm. Chitosan-coated NLCs had a positive surface charge, with zeta potential values ranging from 45.8 mV to 59.0 mV. Exposure of L929 cells to NLCs resulted in over 70 % cell viability. Conversely, surface modification significantly reduced the viability of promastigotes (93 %) compared to free compounds. Moreover, chitosan-coated NLCs presented a better IC Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Chitosan; Essential Oil; Leishmania; Monoterpene; Nanocarriers; Nanomedicine; Surface Modification |
| المشرفين على المادة: | 0 (Oils, Volatile) 9MC3I34447 (Limonene) 0 (Drug Carriers) 9012-76-4 (Chitosan) 0 (Lipids) 0 (Antiprotozoal Agents) 0 (Terpenes) |
| تواريخ الأحداث: | Date Created: 20240721 Date Completed: 20240823 Latest Revision: 20240823 |
| رمز التحديث: | 20240826 |
| DOI: | 10.1016/j.ijpharm.2024.124464 |
| PMID: | 39033939 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-3476 |
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| DOI: | 10.1016/j.ijpharm.2024.124464 |