دورية أكاديمية
Next-Generation Sequencing and Proteomics-Enabled Approach for Rapid and High-Throughput Isolation of Virus-Neutralizing Nanobodies.
| العنوان: | Next-Generation Sequencing and Proteomics-Enabled Approach for Rapid and High-Throughput Isolation of Virus-Neutralizing Nanobodies. |
|---|---|
| المؤلفون: | Wu X; State Key Laboratory of Pharmaceutical Biotechnology, Medical School, Nanjing University, Nanjing, China.; Chemistry and Biomedicine Innovation Center, Jiangsu Key Laboratory of Molecular Medicine, Medical School, Nanjing University, Nanjing, China., Zhang J; State Key Laboratory of Pharmaceutical Biotechnology, Medical School, Nanjing University, Nanjing, China.; Chemistry and Biomedicine Innovation Center, Jiangsu Key Laboratory of Molecular Medicine, Medical School, Nanjing University, Nanjing, China., Fang L; State Key Laboratory of Pharmaceutical Biotechnology, Medical School, Nanjing University, Nanjing, China. njfanglei@nju.edu.cn.; Chemistry and Biomedicine Innovation Center, Jiangsu Key Laboratory of Molecular Medicine, Medical School, Nanjing University, Nanjing, China. njfanglei@nju.edu.cn. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2824, pp. 135-146. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | High-Throughput Nucleotide Sequencing*/methods , Proteomics*/methods , Single-Domain Antibodies*/genetics , Single-Domain Antibodies*/immunology , Single-Domain Antibodies*/isolation & purification , Antibodies, Neutralizing*/immunology, Animals ; Camelus ; Antibodies, Viral/immunology ; High-Throughput Screening Assays/methods ; Mass Spectrometry/methods |
| مستخلص: | Compared with traditional antibodies, nanobodies from camelids have various advantages, including small molecular weight, high affinity, low immunogenicity, convenient production through genetic engineering, etc. Here we combined next-generation sequencing (NGS) with proteomics technology based on affinity purification-mass spectrometry (AP-MS) and bioinformatics analysis to high-throughput screen monoclonal nanobodies from camels immunized with surface glycoprotein (glycoprotein N, Gn) of severe fever with thrombocytopenia syndrome virus and fulfilled production of the screened anti-Gn monoclonal nanobody with high affinity by genetic engineering. The innovative high-throughput technical route developed here could also be expanded to the production of neutralizing nanobodies specific for Rift Valley fever virus. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| References: | Muyldermans S (2013) Nanobodies: natural single-domain antibodies. Annu Rev Biochem 82:775–797. https://doi.org/10.1146/annurev-biochem-063011-092449. (PMID: 10.1146/annurev-biochem-063011-09244923495938) Muyldermans S, Baral TN, Retamozzo VC, De Baetselier P, De Genst E, Kinne J, Leonhardt H, Magez S, Nguyen VK, Revets H, Rothbauer U, Stijlemans B, Tillib S, Wernery U, Wyns L, Hassanzadeh-Ghassabeh G, Saerens D (2009) Camelid immunoglobulins and nanobody technology. Vet Immunol Immunopathol 128(1–3):178–183. https://doi.org/10.1016/j.vetimm.2008.10.299. (PMID: 10.1016/j.vetimm.2008.10.29919026455) Steeland S, Vandenbroucke RE, Libert C (2016) Nanobodies as therapeutics: big opportunities for small antibodies. Drug Discov Today 21(7):1076–1113. https://doi.org/10.1016/j.drudis.2016.04.003. (PMID: 10.1016/j.drudis.2016.04.00327080147) Kubala MH, Kovtun O, Alexandrov K, Collins BM (2010) Structural and thermodynamic analysis of the GFP:GFP-nanobody complex. Protein Sci 19(12):2389–2401. https://doi.org/10.1002/pro.519. (PMID: 10.1002/pro.519209453583009406) Liu W, Song H, Chen Q, Yu J, Xian M, Nian R, Feng D (2018) Recent advances in the selection and identification of antigen-specific nanobodies. Mol Immunol 96:37–47. https://doi.org/10.1016/j.molimm.2018.02.012. (PMID: 10.1016/j.molimm.2018.02.01229477934) Bannas P, Hambach J, Koch-Nolte F (2017) Nanobodies and Nanobody-based human heavy chain antibodies as antitumor therapeutics. Front Immunol 8:1603. https://doi.org/10.3389/fimmu.2017.01603. (PMID: 10.3389/fimmu.2017.01603292132705702627) Song P, Zheng N, Liu Y, Tian C, Wu X, Ma X, Chen D, Zou X, Wang G, Wang H, Zhang Y, Lu S, Wu C, Wu Z (2018) Deficient humoral responses and disrupted B-cell immunity are associated with fatal SFTSV infection. Nat Commun 9(1):3328. https://doi.org/10.1038/s41467-018-05746-9. (PMID: 10.1038/s41467-018-05746-9301274396102208) Wu X, Li Y, Huang B, Ma X, Zhu L, Zheng N, Xu S, Nawaz W, Xu C, Wu Z (2020) A single-domain antibody inhibits SFTSV and mitigates virus-induced pathogenesis in vivo. JCI. Insight 5(13). https://doi.org/10.1172/jci.insight.136855. Kurien BT, Scofield RH (2006) Western blotting. Methods 38(4):283–293. https://doi.org/10.1016/j.ymeth.2005.11.007. (PMID: 10.1016/j.ymeth.2005.11.00716483794) Baral TN, MacKenzie R, Arbabi Ghahroudi M (2013) Single-domain antibodies and their utility. Curr Protoc Immunol 103:2.17.11–12.17.57. https://doi.org/10.1002/0471142735.im0217s103. (PMID: 10.1002/0471142735.im0217s103) Grabherr MG, Haas BJ, Yassour M, Levin JZ, Thompson DA, Amit I, Adiconis X, Fan L, Raychowdhury R, Zeng Q, Chen Z, Mauceli E, Hacohen N, Gnirke A, Rhind N, di Palma F, Birren BW, Nusbaum C, Lindblad-Toh K, Friedman N, Regev A (2011) Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol 29(7):644–652. https://doi.org/10.1038/nbt.1883. (PMID: 10.1038/nbt.1883215724403571712) Cheung WC, Beausoleil SA, Zhang X, Sato S, Schieferl SM, Wieler JS, Beaudet JG, Ramenani RK, Popova L, Comb MJ, Rush J, Polakiewicz RD (2012) A proteomics approach for the identification and cloning of monoclonal antibodies from serum. Nat Biotechnol 30(5):447–452. https://doi.org/10.1038/nbt.2167. (PMID: 10.1038/nbt.216722446692) Villén J, Gygi SP (2008) The SCX/IMAC enrichment approach for global phosphorylation analysis by mass spectrometry. Nat Protoc 3(10):1630–1638. https://doi.org/10.1038/nprot.2008.150. (PMID: 10.1038/nprot.2008.150188331992728452) |
| فهرسة مساهمة: | Keywords: Affinity purification-mass spectrometry; Genetic engineering; Neutralizing nanobodies; Next-generation sequencing; Proteomics |
| المشرفين على المادة: | 0 (Single-Domain Antibodies) 0 (Antibodies, Neutralizing) 0 (Antibodies, Viral) |
| تواريخ الأحداث: | Date Created: 20240722 Date Completed: 20240722 Latest Revision: 20240722 |
| رمز التحديث: | 20240723 |
| DOI: | 10.1007/978-1-0716-3926-9_10 |
| PMID: | 39039411 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
|---|---|
| DOI: | 10.1007/978-1-0716-3926-9_10 |