دورية أكاديمية
Identification of RVFV Host Factors Using Quantitative Interaction Proteomics.
العنوان: | Identification of RVFV Host Factors Using Quantitative Interaction Proteomics. |
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المؤلفون: | Wilken L; Leibniz Institute of Virology (LIV), Hamburg, Germany., Lasswitz L; Institute of Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hannover, Germany., Scaturro P; Leibniz Institute of Virology (LIV), Hamburg, Germany. pietro.scaturro@leibniz-liv.de., Gerold G; Institute of Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hannover, Germany. gisa.gerold@tiho-hannover.de.; Department of Clinical Microbiology, Umeå University, Umeå, Sweden. gisa.gerold@tiho-hannover.de.; Wallenberg Centre for Molecular Medicine (WCMM), Umeå University, Umeå, Sweden. gisa.gerold@tiho-hannover.de. |
المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2824, pp. 189-202. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
مواضيع طبية MeSH: | Proteomics*/methods , Tandem Mass Spectrometry*/methods , Host-Pathogen Interactions* , Rift Valley fever virus*, Chromatography, Liquid/methods ; Humans ; Viral Proteins/metabolism ; Rift Valley Fever/virology ; Rift Valley Fever/metabolism ; Animals |
مستخلص: | Affinity enrichment coupled with liquid chromatography-tandem mass spectrometry (AE-LC-MS/MS) enables a comprehensive study of virus-host protein-protein interactions in cells and tissues infected with Rift Valley fever virus (RVFV) or ectopically expressing RVFV proteins. Depending on the research question, different experimental setups with carefully chosen controls are needed. Here, we describe the detailed workflow of sample preparation, processing, and cleanup, while also outlining critical points to consider when designing and performing AE-LC-MS/MS experiments. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
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فهرسة مساهمة: | Keywords: Affinity enrichment; Bottom-up proteomics; Immunoprecipitation; Mass spectrometry; Protein; Protein purification; Virus; host interaction; protein interaction |
المشرفين على المادة: | 0 (Viral Proteins) |
تواريخ الأحداث: | Date Created: 20240722 Date Completed: 20240722 Latest Revision: 20240722 |
رمز التحديث: | 20240723 |
DOI: | 10.1007/978-1-0716-3926-9_13 |
PMID: | 39039414 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1940-6029 |
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DOI: | 10.1007/978-1-0716-3926-9_13 |