دورية أكاديمية
Identification of RVFV Host Factors Using Quantitative Interaction Proteomics.
| العنوان: | Identification of RVFV Host Factors Using Quantitative Interaction Proteomics. |
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| المؤلفون: | Wilken L; Leibniz Institute of Virology (LIV), Hamburg, Germany., Lasswitz L; Institute of Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hannover, Germany., Scaturro P; Leibniz Institute of Virology (LIV), Hamburg, Germany. pietro.scaturro@leibniz-liv.de., Gerold G; Institute of Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hannover, Germany. gisa.gerold@tiho-hannover.de.; Department of Clinical Microbiology, Umeå University, Umeå, Sweden. gisa.gerold@tiho-hannover.de.; Wallenberg Centre for Molecular Medicine (WCMM), Umeå University, Umeå, Sweden. gisa.gerold@tiho-hannover.de. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2824, pp. 189-202. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Proteomics*/methods , Tandem Mass Spectrometry*/methods , Host-Pathogen Interactions* , Rift Valley fever virus*, Chromatography, Liquid/methods ; Humans ; Viral Proteins/metabolism ; Rift Valley Fever/virology ; Rift Valley Fever/metabolism ; Animals |
| مستخلص: | Affinity enrichment coupled with liquid chromatography-tandem mass spectrometry (AE-LC-MS/MS) enables a comprehensive study of virus-host protein-protein interactions in cells and tissues infected with Rift Valley fever virus (RVFV) or ectopically expressing RVFV proteins. Depending on the research question, different experimental setups with carefully chosen controls are needed. Here, we describe the detailed workflow of sample preparation, processing, and cleanup, while also outlining critical points to consider when designing and performing AE-LC-MS/MS experiments. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| References: | Wilson WC, Gaudreault NN, Hossain MM (2015) Rift Valley fever virus. Mol Detect Anim Viral Pathog 5:553–561. https://doi.org/10.2174/156652405774962263. (PMID: 10.2174/156652405774962263) Bird BH, Ksiazek TG, Nichol ST, MacLachlan NJ (2009) Rift Valley fever virus. J Am Vet Med Assoc 234:883–893. https://doi.org/10.2460/javma.234.7.883. (PMID: 10.2460/javma.234.7.88319335238) Simanjuntak Y, Schamoni-Kast K, Grün A, Uetrecht C, Scaturro P (2021) Top-down and bottom-up proteomics methods to study RNA virus biology. Viruses 13:668. https://doi.org/10.3390/v13040668. (PMID: 10.3390/v13040668339243918070632) Gerold G, Bruening J, Pietschmann T (2016) Decoding protein networks during virus entry by quantitative proteomics. Virus Res 218:25–39. https://doi.org/10.1016/J.VIRUSRES.2015.09.006. (PMID: 10.1016/J.VIRUSRES.2015.09.00626365680) Keilhauer EC, Hein MY, Mann M (2015) Accurate protein complex retrieval by affinity enrichment mass spectrometry (AE-MS) rather than affinity purification mass spectrometry (AP-MS). Mol Cell Proteomics 14:120–135. https://doi.org/10.1074/mcp.M114.041012. (PMID: 10.1074/mcp.M114.04101225363814) Lum KK, Cristea IM (2016) Proteomic approaches to uncovering virus-host protein interactions during the progression of viral infection. Expert Rev Proteomics 13:325–340. (PMID: 10.1586/14789450.2016.1147353268176134919574) Nuss JE, Kehn-Hall K, Benedict A, Costantino J, Ward M, Peyser BD, Retterer CJ, Tressler LE, Wanner LM, McGovern HF, Zaidi A, Anthony SM, Kota KP, Bavari S, Hakami RM (2014) Multi-faceted proteomic characterization of host protein complement of Rift Valley fever virus virions and identification of specific heat shock proteins, including HSP90, as important viral host factors. PLoS One 9:e93483. https://doi.org/10.1371/journal.pone.0093483. (PMID: 10.1371/journal.pone.0093483248095074014464) Uckeley ZM, Moeller R, Kühn LI, Nilsson E, Robens C, Lasswitz L, Lindqvist R, Lenman A, Passos V, Voss Y, Sommerauer C, Kampmann M, Goffinet C, Meissner F, Överby AK, Lozach PY, Gerold G (2019) Quantitative proteomics of uukuniemi virus-host cell interactions reveals GBF1 as proviral host factor for phleboviruses. Mol Cell Proteomics 18:2401–2417. https://doi.org/10.1074/mcp.RA119.001631. (PMID: 10.1074/mcp.RA119.001631315704976885706) Zhang L-K, Wang B, Xin Q, Shang W, Shen S, Xiao G, Deng F, Wang H, Hu Z, Wang M (2019) Quantitative proteomic analysis reveals unfolded-protein response involved in severe fever with thrombocytopenia syndrome virus infection. J Virol 93:e00308–e00319. https://doi.org/10.1128/jvi.00308-19. (PMID: 10.1128/jvi.00308-19308423326498065) Harney DJ, Hutchison AT, Hatchwell L, Humphrey SJ, James DE, Hocking S, Heilbronn LK, Larance M (2019) Proteomic analysis of human plasma during intermittent fasting. J Proteome Res 18:2228–2240. https://doi.org/10.1021/acs.jproteome.9b00090. (PMID: 10.1021/acs.jproteome.9b00090308920456503536) Rappsilber J, Mann M, Ishihama Y (2007) Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips. Nat Protoc 2:1896–1906. https://doi.org/10.1038/nprot.2007.261. (PMID: 10.1038/nprot.2007.26117703201) Wiśniewski JR, Zougman A, Mann M (2009) Combination of FASP and StageTip-based fractionation allows in-depth analysis of the hippocampal membrane proteome. J Proteome Res 8:5674–5678. https://doi.org/10.1021/pr900748n. (PMID: 10.1021/pr900748n19848406) Franz T, Li X (2014) Step-by-step preparation of proteins for mass spectrometric analysis. Methods Mol Biol 1295:235–247. https://doi.org/10.1007/978-1-4939-2550-6_18. (PMID: 10.1007/978-1-4939-2550-6_18) |
| فهرسة مساهمة: | Keywords: Affinity enrichment; Bottom-up proteomics; Immunoprecipitation; Mass spectrometry; Protein; Protein purification; Virus; host interaction; protein interaction |
| المشرفين على المادة: | 0 (Viral Proteins) |
| تواريخ الأحداث: | Date Created: 20240722 Date Completed: 20240722 Latest Revision: 20240722 |
| رمز التحديث: | 20240723 |
| DOI: | 10.1007/978-1-0716-3926-9_13 |
| PMID: | 39039414 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-0716-3926-9_13 |