دورية أكاديمية
Cytoskeleton-associated gelsolin responds to the midgut distention process in saline meal-fed Aedes aegypti and affects arbovirus dissemination from the midgut.
| العنوان: | Cytoskeleton-associated gelsolin responds to the midgut distention process in saline meal-fed Aedes aegypti and affects arbovirus dissemination from the midgut. |
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| المؤلفون: | Cui Y; Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri, USA., Megawati D; Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri, USA.; Department of Microbiology and Parasitology, School of Medicine, Warmadewa University, Denpasar, Bali, Indonesia., Lin J; Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri, USA., Rehard DG; Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri, USA., Grant DG; Electron Microscopy Core, University of Missouri, Columbia, Missouri, USA., Liu P; Charles W. Gehrke Proteomics Center, University of Missouri, Columbia, Missouri, USA., Jurkevich A; Advanced Light Microscopy Core, University of Missouri, Columbia, Missouri, USA., Reid WR; Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri, USA., Mooney BP; Charles W. Gehrke Proteomics Center, University of Missouri, Columbia, Missouri, USA., Franz AWE; Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri, USA. |
| المصدر: | FASEB journal : official publication of the Federation of American Societies for Experimental Biology [FASEB J] 2024 Jul 31; Vol. 38 (14), pp. e23764. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Federation of American Societies for Experimental Biology Country of Publication: United States NLM ID: 8804484 Publication Model: Print Cited Medium: Internet ISSN: 1530-6860 (Electronic) Linking ISSN: 08926638 NLM ISO Abbreviation: FASEB J Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2020- : [Bethesda, Md.] : Hoboken, NJ : Federation of American Societies for Experimental Biology ; Wiley Original Publication: [Bethesda, Md.] : The Federation, [c1987- |
| مواضيع طبية MeSH: | Aedes*/virology , Aedes*/metabolism , Gelsolin*/metabolism , Gelsolin*/genetics , Insect Proteins*/metabolism , Insect Proteins*/genetics , Arboviruses*/physiology, Animals ; Cytoskeleton/metabolism ; Female ; Mosquito Vectors/virology ; Mosquito Vectors/metabolism ; Proteomics/methods ; Zika Virus/physiology |
| مستخلص: | The mosquito, Aedes aegypti, is the principal vector for several arboviruses. The mosquito midgut is the initial tissue that gets infected with an arbovirus acquired along with a blood meal from a vertebrate host. Blood meal ingestion leads to midgut tissue distention thereby increasing the pore size of the surrounding basal lamina. This allows newly synthesized virions to exit the midgut by traversing the distended basal lamina to infect secondary tissues of the mosquito. We conducted a quantitative label-free proteomic time course analysis with saline meal-fed Ae. aegypti females to identify host factors involved in midgut tissue distention. Around 2000 proteins were detected during each of the seven sampling time points and 164 of those were uniquely expressed. Forty-five of 97 differentially expressed proteins were upregulated during the 96-h time course and most of those were involved in cytoskeleton modulation, metabolic activity, and vesicle/vacuole formation. The F-actin-modulating Ae. aegypti (Aa)-gelsolin was selected for further functional studies. Stable knockout of Aa-gelsolin resulted in a mosquito line, which showed distorted actin filaments in midgut-associated tissues likely due to diminished F-actin processing by gelsolin. Zika virus dissemination from the midgut of these mosquitoes was diminished and delayed. The loss of Aa-gelsolin function was associated with an increased induction of apoptosis in midgut tissue indicating an involvement of Aa-gelsolin in apoptotic signaling in mosquitoes. Here, we used proteomics to discover a novel host factor, Aa-gelsolin, which affects the midgut escape barrier for arboviruses in mosquitoes and apoptotic signaling in the midgut. (© 2024 The Author(s). The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.) |
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| معلومات مُعتمدة: | R01 AI134661 United States AI NIAID NIH HHS; 1R01AI134661 HHS | National Institutes of Health (NIH) |
| فهرسة مساهمة: | Keywords: Aedes aegypti; CRISPR/Cas9; Zika virus; apoptosis; cytoskeleton; electron microscopy; gelsolin; mosquito; quantitative whole‐proteomic analysis |
| المشرفين على المادة: | 0 (Gelsolin) 0 (Insect Proteins) |
| تواريخ الأحداث: | Date Created: 20240723 Date Completed: 20240723 Latest Revision: 20240823 |
| رمز التحديث: | 20240823 |
| مُعرف محوري في PubMed: | PMC11268798 |
| DOI: | 10.1096/fj.202302684RR |
| PMID: | 39042395 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1530-6860 |
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| DOI: | 10.1096/fj.202302684RR |