دورية أكاديمية
أعرض في EDS

Rapid differentiation of cystic fibrosis-related bacteria via reagentless atmospheric pressure photoionisation mass spectrometry.

التفاصيل البيبلوغرافية
العنوان: Rapid differentiation of cystic fibrosis-related bacteria via reagentless atmospheric pressure photoionisation mass spectrometry.
المؤلفون: Haworth-Duff A; Department of Electrical Engineering and Electronics, University of Liverpool, Liverpool, UK., Smith BL; Department of Electrical Engineering and Electronics, University of Liverpool, Liverpool, UK., Sham TT; Department of Electrical Engineering and Electronics, University of Liverpool, Liverpool, UK., Boisdon C; Department of Electrical Engineering and Electronics, University of Liverpool, Liverpool, UK., Loughnane P; Department of Biochemistry and Systems Biology, University of Liverpool, Liverpool, UK., Burnley M; Department of Electrical Engineering and Electronics, University of Liverpool, Liverpool, UK., Hawcutt DB; Department of Women's and Children's Health, University of Liverpool, Liverpool, UK.; NIHR Alder Hey Clinical Research Facility, Liverpool, UK., Raval R; Open Innovation Hub for Antimicrobial Surfaces, Department of Chemistry, University of Liverpool, Liverpool, UK., Maher S; Department of Electrical Engineering and Electronics, University of Liverpool, Liverpool, UK. s.maher@liverpool.ac.uk.
المصدر: Scientific reports [Sci Rep] 2024 Jul 24; Vol. 14 (1), pp. 17067. Date of Electronic Publication: 2024 Jul 24.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Cystic Fibrosis*/microbiology , Volatile Organic Compounds*/analysis , Pseudomonas aeruginosa*/isolation & purification , Atmospheric Pressure* , Mass Spectrometry*/methods, Humans ; Breath Tests/methods ; Escherichia coli/isolation & purification ; Staphylococcus aureus/isolation & purification
مستخلص: Breath analysis is an area of significant interest in medical research as it allows for non-invasive sampling with exceptional potential for disease monitoring and diagnosis. Volatile organic compounds (VOCs) found in breath can offer critical insight into a person's lifestyle and/or disease/health state. To this end, the development of a rapid, sensitive, cost-effective and potentially portable method for the detection of key compounds in breath would mark a significant advancement. Herein, we have designed, built and tested a novel reagent-less atmospheric pressure photoionisation (APPI) source, coupled with mass spectrometry (MS), utilising a bespoke bias electrode within a custom 3D printed sampling chamber for direct analysis of VOCs. Optimal APPI-MS conditions were identified, including bias voltage, cone voltage and vaporisation temperature. Calibration curves were produced for ethanol, acetone, 2-butanone, ethyl acetate and eucalyptol, yielding R 2  > 0.99 and limits of detection < 10 pg. As a pre-clinical proof of concept, this method was applied to bacterial headspace samples of Escherichia coli (EC), Pseudomonas aeruginosa (PSA) and Staphylococcus aureus (SA) collected in 1 L Tedlar bags. In particular, PSA and SA are commonly associated with lung infection in cystic fibrosis patients. The headspace samples were classified using principal component analysis with 86.9% of the total variance across the first three components and yielding 100% classification in a blind-sample study. All experiments conducted with the novel APPI arrangement were carried out directly in real-time with low-resolution MS, which opens up exciting possibilities in the future for on-site (e.g., in the clinic) analysis with a portable system.
(© 2024. The Author(s).)
References: ERJ Open Res. 2020 Jan 10;6(1):. (PMID: 31956658)
Mass Spectrom Rev. 2003 Sep-Oct;22(5):318-31. (PMID: 12949917)
Sci Rep. 2016 Oct 21;6:35643. (PMID: 27767044)
J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Oct 15;1097-1098:27-34. (PMID: 30199747)
J Breath Res. 2017 Mar 01;11(1):016015. (PMID: 28165332)
J Breath Res. 2013 Mar;7(1):017107. (PMID: 23445880)
J Cyst Fibros. 2022 Jan;21(1):e28-e34. (PMID: 34016557)
Anal Bioanal Chem. 2020 Jun;412(15):3663-3671. (PMID: 32333078)
Anal Chim Acta. 2008 Oct 3;627(1):34-49. (PMID: 18790126)
PLoS One. 2024 Jan 26;19(1):e0297086. (PMID: 38277384)
J Breath Res. 2021 Apr 21;15(3):. (PMID: 33761469)
Am J Respir Crit Care Med. 2002 Jun 15;165(12):1597-601. (PMID: 12070059)
Sci Rep. 2021 Aug 9;11(1):16089. (PMID: 34373549)
Sci Rep. 2020 Dec 9;10(1):21504. (PMID: 33299034)
J Breath Res. 2016 Aug 10;10(3):037102. (PMID: 27506232)
J Breath Res. 2016 Nov 30;10(4):046014. (PMID: 27902490)
Metabolites. 2021 Nov 11;11(11):. (PMID: 34822431)
Sci Rep. 2019 May 29;9(1):7994. (PMID: 31142757)
Mass Spectrom Rev. 2017 May;36(3):423-449. (PMID: 25988849)
Chempluschem. 2020 Jul;85(7):1559-1563. (PMID: 32725968)
Anal Bioanal Chem. 2013 Sep;405(22):7011-8. (PMID: 23180079)
Ann Surg. 2019 May;269(5):903-910. (PMID: 29194085)
J Breath Res. 2022 Sep 12;16(4):. (PMID: 36052728)
Mass Spectrom Rev. 2014 Sep-Oct;33(5):394-413. (PMID: 24272872)
Clin Chem. 1983 Jan;29(1):5-15. (PMID: 6336681)
J Breath Res. 2014 Jun;8(2):027106. (PMID: 24737039)
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Nov 15;875(2):344-8. (PMID: 18938115)
Trends Biotechnol. 2014 Oct;32(10):538-48. (PMID: 25179940)
J Am Soc Mass Spectrom. 2019 Dec;30(12):2584-2593. (PMID: 31713174)
J Thorac Dis. 2013 Oct;5 Suppl 5:S540-50. (PMID: 24163746)
J Air Waste Manag Assoc. 1999 May;49(5):594-8. (PMID: 10352577)
Anal Chem. 2016 Sep 20;88(18):9047-55. (PMID: 27574033)
Anal Chem. 2013 Nov 5;85(21):10321-9. (PMID: 24044609)
Anal Chem. 2008 Nov 1;80(21):8210-5. (PMID: 18821733)
Appl Microbiol Biotechnol. 2019 Nov;103(21-22):9091-9101. (PMID: 31664484)
Sci Rep. 2022 Aug 22;12(1):14308. (PMID: 35995992)
BMC Pulm Med. 2010 Nov 07;10:56. (PMID: 21054900)
J Allergy Clin Immunol. 1998 May;101(5):576-80. (PMID: 9600491)
Metabolomics. 2017;13(10):110. (PMID: 28867989)
J Cyst Fibros. 2014 May;13 Suppl 1:S23-42. (PMID: 24856775)
Anal Chim Acta. 2012 Aug 13;738:69-75. (PMID: 22790702)
J Pharm Biomed Anal. 2017 Aug 5;142:218-224. (PMID: 28521275)
Sci Rep. 2015 Oct 19;5:15163. (PMID: 26477831)
Analyst. 2018 Jul 7;143(13):3148-3155. (PMID: 29878008)
Lung Cancer. 2010 Feb;67(2):227-31. (PMID: 19409642)
Physiol Meas. 2003 Feb;24(1):107-19. (PMID: 12636190)
Anal Chim Acta. 2011 Oct 17;704(1-2):87-97. (PMID: 21907025)
Anal Bioanal Chem. 2017 Sep;409(24):5689-5696. (PMID: 28717894)
Anal Methods. 2021 Nov 25;13(45):5441-5449. (PMID: 34780594)
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Oct 25;810(2):269-75. (PMID: 15380724)
J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Aug 1;879(23):2220-8. (PMID: 21724475)
Metabolomics. 2018;14(5):66. (PMID: 29725275)
Sci Rep. 2020 Jul 1;10(1):10698. (PMID: 32612114)
Lung Cancer. 2009 Feb;63(2):164-8. (PMID: 18599152)
Analyst. 2023 Oct 23;148(21):5366-5379. (PMID: 37702052)
Anal Chim Acta. 2014 May 8;824:1-17. (PMID: 24759744)
Anal Chem. 2020 Sep 1;92(17):11543-11547. (PMID: 32786499)
J Breath Res. 2014 Sep;8(3):037105. (PMID: 25190194)
Sci Rep. 2023 Jan 31;13(1):1768. (PMID: 36720961)
معلومات مُعتمدة: EP/Y003519/1 EPSRC; BB/R012415/1 National Biofilms Innovation Centre
المشرفين على المادة: 0 (Volatile Organic Compounds)
تواريخ الأحداث: Date Created: 20240724 Date Completed: 20240725 Latest Revision: 20240806
رمز التحديث: 20240806
مُعرف محوري في PubMed: PMC11269582
DOI: 10.1038/s41598-024-66851-y
PMID: 39048618
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-024-66851-y