دورية أكاديمية
أعرض في EDS

Antiproliferative effect of low-level laser/ photobiomodulation on gingival fibroblasts derived from calcium channel blocker-induced gingival overgrowth.

التفاصيل البيبلوغرافية
العنوان: Antiproliferative effect of low-level laser/ photobiomodulation on gingival fibroblasts derived from calcium channel blocker-induced gingival overgrowth.
المؤلفون: Slezovic MÖ; Department of Periodontology, University of Health Sciences, Gulhane Faculty of Dental Medicine, Ankara, Turkey. melis.ozgulslezovic@sbu.edu.tr., Saygun I; Department of Periodontology, University of Health Sciences, Gulhane Faculty of Dental Medicine, Ankara, Turkey., Bengi VU; Department of Periodontology, University of Health Sciences, Gulhane Faculty of Dental Medicine, Ankara, Turkey., Serdar M; Department of Medical Biochemistry, Acıbadem Mehmet Ali Aydınlar University, Ankara, Turkey., Kantarci A; Forsyth Institute, Cambridge, Massachusetts, USA.
المصدر: Lasers in medical science [Lasers Med Sci] 2024 Jul 25; Vol. 39 (1), pp. 194. Date of Electronic Publication: 2024 Jul 25.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: England NLM ID: 8611515 Publication Model: Electronic Cited Medium: Internet ISSN: 1435-604X (Electronic) Linking ISSN: 02688921 NLM ISO Abbreviation: Lasers Med Sci Subsets: MEDLINE
أسماء مطبوعة: Publication: London : Springer
Original Publication: London : Baillière Tindall, c1986-
مواضيع طبية MeSH: Fibroblasts*/radiation effects , Fibroblasts*/drug effects , Low-Level Light Therapy*/methods , Gingival Overgrowth*/chemically induced , Gingival Overgrowth*/radiotherapy , Gingival Overgrowth*/therapy , Calcium Channel Blockers*/pharmacology , Cell Proliferation*/radiation effects , Cell Proliferation*/drug effects , Gingiva*/radiation effects , Gingiva*/cytology , Connective Tissue Growth Factor*/metabolism, Humans ; Cells, Cultured ; Collagen Type I/metabolism ; Transforming Growth Factor beta1/metabolism ; Cell Survival/radiation effects ; Cell Survival/drug effects ; Lasers, Semiconductor/therapeutic use ; Male ; Adult ; Female
مستخلص: The aim of this study was to evaluate the antiproliferative properties of low-level laser therapy (LLLT) on gingival fibroblasts obtained from calcium channel blocker-induced gingival overgrowth (GO). Gingival fibroblasts of patients with GO were compared to healthy gingival fibroblasts (H). Both cells were exposed to LLLT (685 nm wavelength, 25mW power, diode laser) and compared to those not treated with LLLT. Cell proliferation and viability were measured with MTT assay at baseline and after 24 and 72 h. TGF-β1, CTGF, and collagen Type 1 levels were evaluated with Enzyme-Linked Immunosorbent Assay (ELISA). LLLT significantly decreased the proliferation of GO fibroblasts (p < 0.05) while leading to a significantly higher proliferation in H fibroblasts compared to the untreated cells (p < 0.05). GO cells showed significantly higher CTGF, TGF-β, and collagen Type 1 expression than the H cells (p < 0.05). LLLT significantly reduced CTGF levels in GO cells compared to the control group (p < 0.05). In H cells, CTGF and TGF-β levels were also significantly decreased in response to LLLT compared to the control group (p < 0.05). While LLLT significantly reduced collagen expression in the H group (p < 0.05), it did not significantly impact the GO cells. LLLT significantly reduced the synthesis of the growth factors and collagen in both groups with an antiproliferative effect on the gingival fibroblasts from calcium channel blocker-induced GO, suggesting that it can offer a therapeutic approach in the clinical management of drug-induced GO, reversing the fibrotic changes.
(© 2024. The Author(s).)
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فهرسة مساهمة: Keywords: CTGF; Calcium channel blockers; Collagen; Gingival enlargement; Low level laser therapy; Photobiomodulation; TGF- β
المشرفين على المادة: 0 (Calcium Channel Blockers)
139568-91-5 (Connective Tissue Growth Factor)
0 (Collagen Type I)
0 (Transforming Growth Factor beta1)
تواريخ الأحداث: Date Created: 20240725 Date Completed: 20240725 Latest Revision: 20240728
رمز التحديث: 20240728
مُعرف محوري في PubMed: PMC11272680
DOI: 10.1007/s10103-024-04122-y
PMID: 39052077
قاعدة البيانات: MEDLINE
الوصف
تدمد:1435-604X
DOI:10.1007/s10103-024-04122-y