دورية أكاديمية
Promoting successful healing of artificial trachea by intratracheal administration of basic fibroblast growth factor†.
| العنوان: | Promoting successful healing of artificial trachea by intratracheal administration of basic fibroblast growth factor†. |
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| المؤلفون: | Murata Y; Department of Thoracic Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan., Yutaka Y; Department of Thoracic Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan., Fukui T; Department of Thoracic Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan., Hirata R; Central Research Laboratories, Kureha Corporation, Fukushima, Japan., Ibi Y; Department of Biomedical Statistics and Bioinformatics, Kyoto University Graduate School of Medicine, Kyoto, Japan., Hidaka Y; Department of Biomedical Statistics and Bioinformatics, Kyoto University Graduate School of Medicine, Kyoto, Japan., Yoshizawa A; Department of Diagnostic Pathology, Nara Medical University, Nara, Japan., Kishimoto Y; Department of Otolaryngology Head and Neck Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan., Omori K; Department of Otolaryngology Head and Neck Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan., Date H; Department of Thoracic Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan. |
| المصدر: | European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery [Eur J Cardiothorac Surg] 2024 Aug 02; Vol. 66 (2). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: Germany NLM ID: 8804069 Publication Model: Print Cited Medium: Internet ISSN: 1873-734X (Electronic) Linking ISSN: 10107940 NLM ISO Abbreviation: Eur J Cardiothorac Surg Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2012-: Oxford, England : Oxford University Press Original Publication: [Berlin] : Springer International ; [Secaucus, NJ, USA : Springer-Verlag New York Inc., distributor, c1987- |
| مواضيع طبية MeSH: | Trachea*/drug effects , Fibroblast Growth Factor 2*/administration & dosage , Fibroblast Growth Factor 2*/pharmacology , Wound Healing*/drug effects, Animals ; Rats ; Male ; Rats, Sprague-Dawley ; Artificial Organs ; Cilia/drug effects |
| مستخلص: | Objectives: This study evaluated the effect of intratracheal administration of basic fibroblast growth factor (bFGF) on tracheal healing following implantation of a novel layered polyglycolic acid (PGA) material to replace a critical-size defect in rat trachea. Methods: A critical-size defect in the rat cervical trachea was covered with PGA. Distilled water (DW) or 3.125, 6.25, 12.5 or 25 µg bFGF was administered into the trachea for 2 weeks (n = 6 for each of 5 groups). Regenerated areas of cilia, ciliary beat frequency and ciliary transport function (CTF) in the centre of the PGA were measured. To examine potential side effects of intratracheal administration of bFGF, the right lower lobe was pathologically evaluated. Results: All rats survived during the study period. Histological examination showed ciliated epithelization on the PGA material after 2 weeks. Bronchoscopy revealed stenosis due to granulation following administration of high concentrations of bFGF (12.5 and 25 µg). Compared with the DW group, groups administered 3.125, 6.25, 12.5 and 25 µg bFGF had significantly larger areas of regenerated cilia (15.2%, 27.0%, 41.3%, 33.1% and 31.0%, respectively; P = 0.00143), improved ciliary beat frequency (7.10, 8.18, 10.10, 9.50 and 9.50 Hz, respectively), and improved CTS (6.40, 9.54, 16.89, 16.41 and 14.29 µm/sec, respectively). Pathological examination of the right lower lobe revealed pulmonary fibrosis and hyperplasia with high concentrations of bFGF (12.5 and 25 µg). Conclusions: Intratracheal administration of bFGF effectively promoted tracheal regeneration at an optimal dose of 6.25 µg following implantation of an artificial trachea. (© The Author(s) 2024. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved.) |
| معلومات مُعتمدة: | Foundation for Research and Promotion of Endoscopy |
| فهرسة مساهمة: | Keywords: Polyglycolic acid; Regeneration; Trachea |
| المشرفين على المادة: | 103107-01-3 (Fibroblast Growth Factor 2) |
| تواريخ الأحداث: | Date Created: 20240725 Date Completed: 20240807 Latest Revision: 20240807 |
| رمز التحديث: | 20240807 |
| DOI: | 10.1093/ejcts/ezae284 |
| PMID: | 39052855 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-734X |
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| DOI: | 10.1093/ejcts/ezae284 |