دورية أكاديمية
Identification and characterization of Staphylococcus argenteus from Indonesia.
| العنوان: | Identification and characterization of Staphylococcus argenteus from Indonesia. |
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| المؤلفون: | Supriadi IR; National Biomedical and Genome Science Institute, Directorate of Healthcare Services, Ministry of Health of Indonesia, Jakarta, Indonesia; Department of Medical Microbiology and Infectious Diseases, Erasmus MC University Medical Center, Rotterdam, the Netherlands; Department of Cardiology, University Medical Centre Utrecht, the Netherlands., Santosaningsih D; Department of Clinical Microbiology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia., Budayanti NS; Department of Microbiology, Faculty of Medicine, Universitas Udayana/Udayana University Hospital, Badung, Indonesia., Zandijk WHA; Department of Medical Microbiology and Infectious Diseases, Erasmus MC University Medical Center, Rotterdam, the Netherlands., Rijfkogel A; Department of Medical Microbiology and Infectious Diseases, Erasmus MC University Medical Center, Rotterdam, the Netherlands., Klaassen CHW; Department of Medical Microbiology and Infectious Diseases, Erasmus MC University Medical Center, Rotterdam, the Netherlands., Severin JA; Department of Medical Microbiology and Infectious Diseases, Erasmus MC University Medical Center, Rotterdam, the Netherlands. Electronic address: j.severin@erasmusmc.nl. |
| المصدر: | International journal of medical microbiology : IJMM [Int J Med Microbiol] 2024 Sep; Vol. 316, pp. 151629. Date of Electronic Publication: 2024 Jul 14. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Urban & Fischer Verlag Country of Publication: Germany NLM ID: 100898849 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-0607 (Electronic) Linking ISSN: 14384221 NLM ISO Abbreviation: Int J Med Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Jena, Germany : Urban & Fischer Verlag, c2000- |
| مواضيع طبية MeSH: | Staphylococcus*/genetics , Staphylococcus*/isolation & purification , Staphylococcus*/classification , Staphylococcus*/drug effects , Staphylococcal Infections*/microbiology , Staphylococcal Infections*/epidemiology , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization* , Microbial Sensitivity Tests* , Anti-Bacterial Agents*/pharmacology , Whole Genome Sequencing*, Indonesia/epidemiology ; Humans ; Male ; Female ; Adult ; Multilocus Sequence Typing ; Middle Aged ; Young Adult ; Soft Tissue Infections/microbiology ; Soft Tissue Infections/epidemiology ; Genome, Bacterial/genetics ; Aged ; Genetic Variation ; Adolescent ; Real-Time Polymerase Chain Reaction |
| مستخلص: | Background: In 2015, Staphylococcus argenteus was reported for the first time as a novel species of the Staphylococcus aureus complex. While S. argenteus has been found in many countries, its presence in Indonesia has not been reported yet. Our aim is to confirm S. argenteus presence in Indonesia, describe its characteristics and analyze its genomic diversity. Methods: The S. aureus isolates used in this study were collected from patients with skin and soft tissue infections in Indonesia, between July 2009 to February 2010. Randomly selected isolates were recultured from -80 C° stocks and analyzed using matrix-assisted laser desorption/ionization - time of flight (MALDI-TOF). Isolates identified as S. argenteus, S. roterodami, or S. schweitzeri and S. aureus with a low score in the MALDI-TOF analysis were analyzed by a real-time PCR targeting the nucA gene able to identify true S. argenteus. Isolates identified as S. argenteus were further characterized by whole genome sequencing. Vitek®2 (bioMérieux) was used for antimicrobial susceptibility testing. Results: Fifteen isolates were identified as S. argenteus, with the majority belonging to ST2250. Two pairs of isolates proved to be identical by core genome multilocus sequence typing analysis. Most isolates were susceptible to all antibiotics tested, except for seven isolates (46.7 %) that were resistant to benzylpenicillin, and one isolate was resistant to tetracycline (6.7 %). The presence of resistance genes blaZ and tet(45) correlated with these findings. Notably, the sey enterotoxin gene was prevalent in 80 % of the isolates. Other virulence factor genes were less prevalent. Plasmid replicon types in S. argenteus were also known to S. aureus. Conclusion: Our study reveals the occurrence of S. argenteus in Indonesia. The diversity within Indonesian S. argenteus matches the global diversity of S. argenteus. Identical isolates between patients indicate potential transmission events. A lower prevalence of a broad panel of virulence factors suggests that S. argenteus is less virulent than S. aureus. Competing Interests: Competing interest All authors declare that they have no competing interest. (Copyright © 2024 The Authors. Published by Elsevier GmbH.. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Indonesia; Infectious skin diseases; Staphylococcus; Virulence; Whole genome sequencing |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) |
| SCR Organism: | Staphylococcus argenteus |
| تواريخ الأحداث: | Date Created: 20240725 Date Completed: 20240916 Latest Revision: 20240916 |
| رمز التحديث: | 20240917 |
| DOI: | 10.1016/j.ijmm.2024.151629 |
| PMID: | 39053073 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1618-0607 |
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| DOI: | 10.1016/j.ijmm.2024.151629 |