دورية أكاديمية
Effect of Lactiplantibacillus plantarum on the growth, hemato-biochemical, inflammation, apoptosis, oxidative stress markers, involved gens and histopathological alterations in growing rabbits challenged with aflatoxin B1.
| العنوان: | Effect of Lactiplantibacillus plantarum on the growth, hemato-biochemical, inflammation, apoptosis, oxidative stress markers, involved gens and histopathological alterations in growing rabbits challenged with aflatoxin B1. |
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| المؤلفون: | Saghir SAM; Department of Medical Analysis, Princess Aisha Bint Al-Hussein College of Nursing and Medical Sciences, Al-Hussein Bin Talal University, Ma'an 71111, Jordan. Electronic address: sultan.s.ayesh@ahu.edu.jo., Al Hroob AM; Department of Medical Analysis, Princess Aisha Bint Al-Hussein College of Nursing and Medical Sciences, Al-Hussein Bin Talal University, Ma'an 71111, Jordan., Al-Tarawni AH; Department of Biological Sciences, Mutah University, Mutah, Al-Karak 61710, Jordan., Abdulghani MAM; Pharmacology Department, International Medical School Management and Science University, Shah Alam, Selangor 40100, Malaysia., Tabana Y; Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton AB T6G 2E1, Canada; Department of Biochemistry, University of Alberta, Edmonton, AB, Canada., Aldhalmi AK; College of Pharmacy, Al- Mustaqbal University, 51001 Babylon, Iraq., Mothana RA; Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia., Al-Yousef HM; Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia. |
| المصدر: | Poultry science [Poult Sci] 2024 Sep; Vol. 103 (9), pp. 104002. Date of Electronic Publication: 2024 Jun 22. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: England NLM ID: 0401150 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1525-3171 (Electronic) Linking ISSN: 00325791 NLM ISO Abbreviation: Poult Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2020- : [Cambridge, UK] : Elsevier Original Publication: Champaign Il : Poultry Science Association |
| مواضيع طبية MeSH: | Aflatoxin B1*/toxicity , Oxidative Stress*/drug effects , Animal Feed*/analysis , Probiotics*/administration & dosage , Probiotics*/pharmacology , Inflammation*/veterinary , Inflammation*/chemically induced , Diet*/veterinary, Animals ; Rabbits ; Male ; Apoptosis/drug effects ; Random Allocation |
| مستخلص: | Aflatoxin B1 (AFB1) is a significant pollutant found in food and feed, posing a threat to public health. The objective of this study was to assess the effect of Lactiplantibacillus plantarum (LACP) against AFB1 in growing rabbits by investigating growth, serum metabolites, immunity, antioxidant capacity, and inflammatory response. A total of 60 growing male rabbits (721.5 ± 2.68g) were allocated to 4 experimental groups. The control group receiving only a basal diet, the AFB1 group (0.3 mg AFB1/kg diet), the LACP group (1 × 10 9 cfu/g /kg diet), and the combination group (1 × 10 9 cfu/g + 0.3 mg AFB1/kg diet; AFB1+ LACP) for 8 wk. The administration of AFB1 alone significantly decreased the final body weight, body gain, and feed intake, while significantly increasing the feed conversion ratio (P < 0.05). A significant decline in total proteins and globulins, along with elevated levels of hepatic enzymes (AST, ALP, ALT, and GGT) and renal function markers (creatinine and uric acid), were observed in the AFB1-contaminated group (P < 0.05). Immunoglobulins (IgG and IgM) were significantly decreased, alongside a significant elevation of triglycerides, direct bilirubin, and indirect bilirubin in growing rabbits fed diets with AFB1 (P < 0.05). Supplementing the AFB1 diet with LACP restored the growth reduction, improved liver (AST, ALP, ALT, and GGT) and kidney (creatinine and uric acid) functions, and enhanced immune markers in rabbit serum (P < 0.05). Antioxidant indices (SOD, GSH, and CAT) were significantly decreased in the AFB1 group (P < 0.05). However, the addition of LACP to the AFB1-contaminated diets improved antioxidant capacity and malondialdehyde (MDA) and protein carbonylation (PC) in hepatic tissues of rabbits (P < 0.05). Serum interlukin-4 (IL-4) and interferon gamma (IFN-γ) levels were significantly increased in the AFB1 group (P < 0.05), but the addition of LACP significantly reversed this elevation. AFB1 downregulated the expression of immune-inflammatory genes such Nrf2, IL-10, and BCL-2 genes, while up-regulating the caspase-3 (CASP3) gene (P < 0.05). Supplementing AFB1 diet with LACP significantly decreased the expression of immune-inflammatory genes (Nrf2, IL-10, and BCL-2) and reduced the expression of the apoptotic-related gene CASP3. This study highlights the potential of L. plantarum (1 × 10 9 cfu/g /kg diet) as a protective agent against AFB1 in growing rabbits by enhancing antioxidant and immune function and reducing apoptosis and inflammation pathways. Competing Interests: DISCLOSURES The authors declare no conflicts of interest. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: aflatoxin B1; apoptosis; inflammation; oxidative stress and immunity; probiotic; rabbit |
| المشرفين على المادة: | 9N2N2Y55MH (Aflatoxin B1) |
| تواريخ الأحداث: | Date Created: 20240725 Date Completed: 20240824 Latest Revision: 20240824 |
| رمز التحديث: | 20240826 |
| مُعرف محوري في PubMed: | PMC11320459 |
| DOI: | 10.1016/j.psj.2024.104002 |
| PMID: | 39053371 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1525-3171 |
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| DOI: | 10.1016/j.psj.2024.104002 |