دورية أكاديمية
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Inositol Restores Appropriate Steroidogenesis in PCOS Ovaries Both In Vitro and In Vivo Experimental Mouse Models.

التفاصيل البيبلوغرافية
العنوان: Inositol Restores Appropriate Steroidogenesis in PCOS Ovaries Both In Vitro and In Vivo Experimental Mouse Models.
المؤلفون: Fedeli V; Department of Experimental Medicine, University La Sapienza, 00185 Rome, Italy.; Systems Biology Group Lab, University La Sapienza, 00185 Rome, Italy.; The Experts Group on Inositol in Basic and Clinical Research, and on PCOS (EGOI-PCOS), 00161 Rome, Italy., Unfer V; The Experts Group on Inositol in Basic and Clinical Research, and on PCOS (EGOI-PCOS), 00161 Rome, Italy.; Dept. of Gynaecology, UniCamillus-Saint Camillus International University of Health Sciences, 00131 Rome, Italy., Dinicola S; Systems Biology Group Lab, University La Sapienza, 00185 Rome, Italy.; The Experts Group on Inositol in Basic and Clinical Research, and on PCOS (EGOI-PCOS), 00161 Rome, Italy., Laganà AS; Unit of Obstetrics and Gynecology, 'Paolo Giaccone' Hospital, Department of Health Promotion, Mother and ChildCare, Internal Medicine and Medical Specialties (PROMISE), University of Palermo, 90127 Palermo, Italy., Canipari R; Department of Anatomy, Histology, Forensic Medicine and Orthopedic, Unit of Histology and Medical Embryology, Sapienza, University of Rome, 00185 Rome, Italy., Monti N; Department of Experimental Medicine, University La Sapienza, 00185 Rome, Italy.; Systems Biology Group Lab, University La Sapienza, 00185 Rome, Italy., Querqui A; Department of Experimental Medicine, University La Sapienza, 00185 Rome, Italy.; Systems Biology Group Lab, University La Sapienza, 00185 Rome, Italy., Galante E; Department of Experimental Medicine, University La Sapienza, 00185 Rome, Italy.; Systems Biology Group Lab, University La Sapienza, 00185 Rome, Italy., Laurenzi G; Department of Anatomy, Histology, Forensic Medicine and Orthopedic, Unit of Histology and Medical Embryology, Sapienza, University of Rome, 00185 Rome, Italy., Bizzarri M; Department of Experimental Medicine, University La Sapienza, 00185 Rome, Italy.; Systems Biology Group Lab, University La Sapienza, 00185 Rome, Italy.; The Experts Group on Inositol in Basic and Clinical Research, and on PCOS (EGOI-PCOS), 00161 Rome, Italy.
المصدر: Cells [Cells] 2024 Jul 09; Vol. 13 (14). Date of Electronic Publication: 2024 Jul 09.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101600052 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4409 (Electronic) Linking ISSN: 20734409 NLM ISO Abbreviation: Cells Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI
مواضيع طبية MeSH: Polycystic Ovary Syndrome*/metabolism , Polycystic Ovary Syndrome*/pathology , Polycystic Ovary Syndrome*/drug therapy , Inositol*/pharmacology , Disease Models, Animal* , Aromatase*/metabolism , Aromatase*/genetics , Receptors, FSH*/metabolism , Receptors, FSH*/genetics , Ovary*/metabolism , Ovary*/drug effects , Ovary*/pathology, Female ; Animals ; Mice ; Granulosa Cells/metabolism ; Granulosa Cells/drug effects ; Theca Cells/metabolism ; Theca Cells/drug effects ; Steroids/biosynthesis
مستخلص: Androgen excess is a key feature of several clinical phenotypes of polycystic ovary syndrome (PCOS). However, the presence of FSH receptor (FSHR) and aromatase (CYP19A1) activity responses to physiological endocrine stimuli play a critical role in the pathogenesis of PCOS. Preliminary data suggest that myo-Inositol (myo-Ins) and D-Chiro-Inositol (D-Chiro-Ins) may reactivate CYP19A1 activity. We investigated the steroidogenic pathway of Theca (TCs) and Granulosa cells (GCs) in an experimental model of murine PCOS induced in CD1 mice exposed for 10 weeks to a continuous light regimen. The effect of treatment with different combinations of myo-Ins and D-Chiro-Ins on the expression of Fshr , androgenic, and estrogenic enzymes was analyzed by real-time PCR in isolated TCs and GCs and in ovaries isolated from healthy and PCOS mice. Myo-Ins and D-Chiro-Ins, at a ratio of 40:1 at pharmacological and physiological concentrations, positively modulate the steroidogenic activity of TCs and the expression of Cyp19a1 and Fshr in GCs. Moreover, in vivo, inositols (40:1 ratio) significantly increase Cyp19a1 and Fshr . These changes in gene expression are mirrored by modifications in hormone levels in the serum of treated animals. Myo-Ins and D-Chiro-Ins in the 40:1 formula efficiently rescued PCOS features by up-regulating aromatase and FSHR levels while down-regulating androgen excesses produced by TCs.
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معلومات مُعتمدة: n. RG123188AEFD2C0D Sapienza University of Rome
فهرسة مساهمة: Keywords: D-chiro-Inositol; FSH receptor; aromatase; myo-Inositol; polycystic ovary syndrome (PCOS); steroidogenesis
المشرفين على المادة: 4L6452S749 (Inositol)
EC 1.14.14.1 (Aromatase)
0 (Receptors, FSH)
0 (Steroids)
EC 1.14.14.1 (Cyp19a1 protein, mouse)
تواريخ الأحداث: Date Created: 20240726 Date Completed: 20240726 Latest Revision: 20240728
رمز التحديث: 20240728
مُعرف محوري في PubMed: PMC11275052
DOI: 10.3390/cells13141171
PMID: 39056753
قاعدة البيانات: MEDLINE
الوصف
تدمد:2073-4409
DOI:10.3390/cells13141171