دورية أكاديمية
أعرض في EDS

Characterisation of Canine and Feline Breast Tumours, Their Metastases, and Corresponding Primary Cell Lines Using LA-REIMS and DESI-MS Imaging.

التفاصيل البيبلوغرافية
العنوان: Characterisation of Canine and Feline Breast Tumours, Their Metastases, and Corresponding Primary Cell Lines Using LA-REIMS and DESI-MS Imaging.
المؤلفون: Molnár A; Hevesy György PhD School of Chemistry, ELTE Eötvös Loránd University, H-1117 Budapest, Hungary.; Waters Research Center, H-1031 Budapest, Hungary.; MTA-ELTE Lendület (Momentum) Ion Mobility Mass Spectrometry Research Group, Faculty of Science, Institute of Chemistry, ELTE Eötvös Loránd University, H-1117 Budapest, Hungary., Horkovics-Kováts GS; Hevesy György PhD School of Chemistry, ELTE Eötvös Loránd University, H-1117 Budapest, Hungary.; Waters Research Center, H-1031 Budapest, Hungary., Kucsma N; Institute of Molecular Life Sciences, HUN-REN Research Centre for Natural Sciences, H-1117 Budapest, Hungary., Szegő Z; Waters Research Center, H-1031 Budapest, Hungary., Tauber B; Qamcom Central Europe, H-1052 Budapest, Hungary., Egri A; Waters Research Center, H-1031 Budapest, Hungary., Szkupien Z; Independent Researcher, H-1141 Budapest, Hungary., Deák BA; Department of Pathology, Forensic and Insurance Medicine, Semmelweis University, H-1085 Budapest, Hungary., McKenzie JS; Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, London W12 0NN, UK., Thuróczy J; Animal Health Center Budafok, H-1221 Budapest, Hungary., Schäffer R; Waters Research Center, H-1031 Budapest, Hungary., Schlosser G; MTA-ELTE Lendület (Momentum) Ion Mobility Mass Spectrometry Research Group, Faculty of Science, Institute of Chemistry, ELTE Eötvös Loránd University, H-1117 Budapest, Hungary., Szakács G; Institute of Molecular Life Sciences, HUN-REN Research Centre for Natural Sciences, H-1117 Budapest, Hungary.; Center for Cancer Research, Medical University of Vienna, 1090 Vienna, Austria., Balog J; Waters Research Center, H-1031 Budapest, Hungary.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2024 Jul 15; Vol. 25 (14). Date of Electronic Publication: 2024 Jul 15.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Mammary Neoplasms, Animal*/pathology , Mammary Neoplasms, Animal*/metabolism , Breast Neoplasms*/pathology , Breast Neoplasms*/metabolism, Cats ; Animals ; Female ; Dogs ; Cell Line, Tumor ; Cat Diseases/pathology ; Spectrometry, Mass, Electrospray Ionization/methods ; Neoplasm Metastasis ; Dog Diseases/pathology ; Dog Diseases/metabolism ; Lipidomics/methods
مستخلص: Breast cancer, a complex disease with a significant prevalence to form metastases, necessitates novel therapeutic strategies to improve treatment outcomes. Here, we present the results of a comparative molecular study of primary breast tumours, their metastases, and the corresponding primary cell lines using Desorption Electrospray Ionisation (DESI) and Laser-Assisted Rapid Evaporative Ionisation Mass Spectrometry (LA-REIMS) imaging. Our results show that ambient ionisation mass spectrometry technology is suitable for rapid characterisation of samples, providing a lipid- and metabolite-rich spectrum within seconds. Our study demonstrates that the lipidomic fingerprint of the primary tumour is not significantly distinguishable from that of its metastasis, in parallel with the similarity observed between their respective primary cell lines. While significant differences were observed between tumours and the corresponding cell lines, distinct lipidomic signatures and several phospholipids such as PA(36:2), PE(36:1), and PE(P-38:4)/PE(O-38:5) for LA-REIMS imaging and PE(P-38:4)/PE(O-38:5), PS(36:1), and PI(38:4) for DESI-MSI were identified in both tumours and cells. We show that the tumours' characteristics can be found in the corresponding primary cell lines, offering a promising avenue for assessing tumour responsiveness to therapeutic interventions. A comparative analysis by DESI-MSI and LA-REIMS imaging revealed complementary information, demonstrating the utility of LA-REIMS in the molecular imaging of cancer.
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معلومات مُعتمدة: RRF-2.3.1-21-2022-00015 European Union; SNN 138407 Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund; J3-3066 Slovenian Research Agency
فهرسة مساهمة: Keywords: ambient ionisation; cancer research; lipidomics; mass spectrometry imaging; metabolic fingerprints; primary cell lines
تواريخ الأحداث: Date Created: 20240727 Date Completed: 20240728 Latest Revision: 20240729
رمز التحديث: 20240729
مُعرف محوري في PubMed: PMC11277125
DOI: 10.3390/ijms25147752
PMID: 39062995
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms25147752