دورية أكاديمية
أعرض في EDS

Analysis of Human Milk Microbiota in Northern Greece by Comparative 16S rRNA Sequencing vs. Local Dairy Animals.

التفاصيل البيبلوغرافية
العنوان: Analysis of Human Milk Microbiota in Northern Greece by Comparative 16S rRNA Sequencing vs. Local Dairy Animals.
المؤلفون: Tsifintaris M; Department of Molecular Biology & Genetics, Democritus University of Thrace, 68100 Alexandroupolis, Greece., Sitmalidis M; Department of Molecular Biology & Genetics, Democritus University of Thrace, 68100 Alexandroupolis, Greece., Tokamani M; Department of Molecular Biology & Genetics, Democritus University of Thrace, 68100 Alexandroupolis, Greece., Anastasiadi C; Department of Molecular Biology & Genetics, Democritus University of Thrace, 68100 Alexandroupolis, Greece., Georganta M; Department of Obstetrics and Gynecology, Democritus University of Thrace, 68100 Alexandroupolis, Greece., Tsochantaridis I; Department of Molecular Biology & Genetics, Democritus University of Thrace, 68100 Alexandroupolis, Greece., Vlachakis D; Department of Biotechnology, Agricultural University of Athens, 11855 Athens, Greece., Tsikouras P; Department of Obstetrics and Gynecology, Democritus University of Thrace, 68100 Alexandroupolis, Greece., Nikolettos N; Department of Obstetrics and Gynecology, Democritus University of Thrace, 68100 Alexandroupolis, Greece., Chrousos GP; University Research Institute of Maternal and Child Health and Precision Medicine, National and Kapodistrian University of Athens, 11527 Athens, Greece.; UNESCO Chair of Adolescent Health, National and Kapodistrian University of Athens, 11527 Athens, Greece., Sandaltzopoulos R; Department of Molecular Biology & Genetics, Democritus University of Thrace, 68100 Alexandroupolis, Greece., Giannakakis A; Department of Molecular Biology & Genetics, Democritus University of Thrace, 68100 Alexandroupolis, Greece.
المصدر: Nutrients [Nutrients] 2024 Jul 09; Vol. 16 (14). Date of Electronic Publication: 2024 Jul 09.
نوع المنشور: Journal Article; Comparative Study
اللغة: English
بيانات الدورية: Publisher: MDPI Publishing Country of Publication: Switzerland NLM ID: 101521595 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6643 (Electronic) Linking ISSN: 20726643 NLM ISO Abbreviation: Nutrients Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI Publishing
مواضيع طبية MeSH: Milk, Human*/microbiology , Milk, Human*/chemistry , RNA, Ribosomal, 16S*/genetics , Microbiota* , Goats* , Colostrum*/microbiology , Milk*/microbiology, Animals ; Humans ; Greece ; Female ; Cattle ; Bacteria/classification ; Bacteria/genetics ; Bacteria/isolation & purification
مستخلص: Milk is a biological fluid with a dynamic composition of micronutrients and bioactive molecules that serves as a vital nutrient source for infants. Milk composition is affected by multiple factors, including genetics, geographical location, environmental conditions, lactation phase, and maternal nutrition, and plays a key role in dictating its microbiome. This study addresses a less-explored aspect, comparing the microbial communities in human breast milk with those in mature milk from species that are used for milk consumption. Since mature animal milk is used as a supplement for both the infant (formula) and the child/adolescent, our main aim was to identify shared microbial communities in colostrum and mature human milk. Using 16S rRNA metagenomic sequencing, we focused on characterizing the milk microbiota in the Northern Greek population by identifying shared microbial communities across samples and comparing the relative abundance of prevalent genera. We analyzed ten human milk samples (from five mothers), with five collected three days postpartum (colostrum) and five collected thirty to forty days postpartum (mature milk) from corresponding mothers. To perform an interspecies comparison of human milk microbiota, we analyzed five goat and five bovine milk samples from a local dairy industry, collected fifty to seventy days after birth. Alpha diversity analysis indicated moderate diversity and stability in bovine milk, high richness in goat milk, and constrained diversity in breast milk. Beta diversity analysis revealed significant distinctions among mammalian species, emphasizing both presence/absence and abundance-based clustering. Despite noticeable differences, shared microbial components underscore fundamental aspects across all mammalian species, highlighting the presence of a core microbiota predominantly comprising the Proteobacteria, Firmicutes, and Actinobacteriota phyla. At the genus level, Acinetobacter , Gemella , and Sphingobium exhibit significant higher abundance in human milk compared to bovine and goat milk, while Pseudomonas and Atopostipes are more prevalent in animal milk. Our comparative analysis revealed differences and commonalities in the microbial communities of various mammalian milks and unraveled the existence of a common fundamental milk core microbiome. We thus revealed both species-specific and conserved microbial communities in human, bovine, and goat milk. The existence of a common core microbiome with conserved differences between colostrum and mature human milk underscores fundamental similarities in the microbiota of milk across mammalian species, which could offer valuable implications for optimizing the nutritional quality and safety of dairy products as well as supplements for infant health.
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معلومات مُعتمدة: 05704 Hellenic Foundation for Research and Innovation
فهرسة مساهمة: Keywords: 16S rRNA amplicon sequencing; bovine milk; goat milk; human milk; metagenomics; milk microbiota
المشرفين على المادة: 0 (RNA, Ribosomal, 16S)
تواريخ الأحداث: Date Created: 20240727 Date Completed: 20240728 Latest Revision: 20240729
رمز التحديث: 20240729
مُعرف محوري في PubMed: PMC11280067
DOI: 10.3390/nu16142175
PMID: 39064618
قاعدة البيانات: MEDLINE
الوصف
تدمد:2072-6643
DOI:10.3390/nu16142175