دورية أكاديمية
أعرض في EDS

Identification and Characterization of a Novel Prophage Lysin against Streptococcus dysgalactiae .

التفاصيل البيبلوغرافية
العنوان: Identification and Characterization of a Novel Prophage Lysin against Streptococcus dysgalactiae .
المؤلفون: Xu L; Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.; College of Agriculture and Forestry, Linyi University, Linyi 276005, China., Li X; College of Agriculture and Forestry, Linyi University, Linyi 276005, China., Yang X; College of Agriculture and Forestry, Linyi University, Linyi 276005, China., Zhao Y; Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.; College of Agriculture and Forestry, Linyi University, Linyi 276005, China., Niu J; College of Agriculture and Forestry, Linyi University, Linyi 276005, China., Jiang S; Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China., Ma J; College of Agriculture and Forestry, Linyi University, Linyi 276005, China., Zhang X; College of Agriculture and Forestry, Linyi University, Linyi 276005, China.
المصدر: Molecules (Basel, Switzerland) [Molecules] 2024 Jul 20; Vol. 29 (14). Date of Electronic Publication: 2024 Jul 20.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, c1995-
مواضيع طبية MeSH: Streptococcus*/drug effects , Prophages*/genetics , Anti-Bacterial Agents*/pharmacology , Anti-Bacterial Agents*/chemistry, Animals ; Milk/microbiology ; Streptococcus Phages/genetics ; Cattle ; Mastitis, Bovine/microbiology ; Mastitis, Bovine/drug therapy ; Hydrogen-Ion Concentration ; Bacteriolysis/drug effects ; Streptococcal Infections/microbiology ; Streptococcal Infections/drug therapy
مستخلص: Streptococcus dysgalactiae infection can cause bovine mastitis and lead to huge economic losses for the dairy industry. The abuse of antibiotics has resulted in growing drug resistance of S. dysgalactiae, which causes hard-to-treat infections. Bacteriophage lysin, as a novel antibacterial agent, has great potential for application against drug-resistant gram-positive bacteria. However, few studies have been conducted on the prophage lysin of S. dysgalactiae . In this study, we mined a novel prophage lysin, named Lys1644, from a clinical S. dysgalactiae isolate by genome sequencing and bioinformatic analysis. Lys1644 was expressed and purified, and the lytic activity, antibacterial spectrum, optimal pH and temperature, lytic activity in milk in vitro, and synergistic bacteriostasis with antibiotics were assessed. The Lys1644 prophage lysin showed high bacteriolysis activity specifically on S. dysgalactiae , which resulted in CFU 100-fold reduction in milk. Moreover, Lys1644 maintained high activity over a wide pH range (pH 5-10) and a wide temperature range (4-42 °C). Synergistic bacteriostatic experiments showed that the combination of low-dose Lys1644 (50 μg/mL) with a subinhibitory concentration of aminoglycoside antibiotics (kanamycin or spectinomycin) can completely inhibit bacterial growth, suggesting that the combination of Lys1644 and antibiotics could be an effective therapeutic strategy against S. dysgalactiae infection.
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معلومات مُعتمدة: 2022TSGC2524 Project of Shandong Province science and technology smes innovation ability improvement; ts20190955 the Taishan Scholars Program of Shandong Province, China; ZR2019ZD21 Natural Science Foundation of Shandong Province, China; 2023TSGC0419 the Project of High-Tech SMES Innovation Improvement of Shandong Province; 2019YFE0103900 the National Key Research and Development Program of China
فهرسة مساهمة: Keywords: Lys1644; Streptococcus dysgalactiae; prophage lysin; synergistic bacteriostasis
المشرفين على المادة: 0 (Anti-Bacterial Agents)
SCR Organism: Streptococcus dysgalactiae
تواريخ الأحداث: Date Created: 20240727 Date Completed: 20240727 Latest Revision: 20240813
رمز التحديث: 20240813
مُعرف محوري في PubMed: PMC11279900
DOI: 10.3390/molecules29143411
PMID: 39064988
قاعدة البيانات: MEDLINE
الوصف
تدمد:1420-3049
DOI:10.3390/molecules29143411