دورية أكاديمية
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Comparative Proteomic Analysis of Huh7 Cells Transfected with Sub-Saharan African Hepatitis B Virus (Sub)genotypes Reveals Potential Oncogenic Factors.

التفاصيل البيبلوغرافية
العنوان: Comparative Proteomic Analysis of Huh7 Cells Transfected with Sub-Saharan African Hepatitis B Virus (Sub)genotypes Reveals Potential Oncogenic Factors.
المؤلفون: Padarath K; Hepatitis Virus Diversity Unit, Department of Internal Medicine, School of Clinical Medicine, Faculty of Health Science, University of Witwatersrand, 7 York Road, Parktown, Johannesburg 2193, South Africa., Deroubaix A; Hepatitis Virus Diversity Unit, Department of Internal Medicine, School of Clinical Medicine, Faculty of Health Science, University of Witwatersrand, 7 York Road, Parktown, Johannesburg 2193, South Africa.; Life Sciences Imaging Facility, Faculty of Health Sciences, University of the Witwatersrand, 7 York Road, Parktown, Johannesburg 2193, South Africa., Naicker P; Future Production Chemicals, Council for Scientific and Industrial Research, Pretoria 0184, South Africa., Stoychev S; ReSyn Biosciences, Johannesburg 2000, South Africa.; Evosep Biosystems, 5230 Odense, Denmark., Kramvis A; Hepatitis Virus Diversity Unit, Department of Internal Medicine, School of Clinical Medicine, Faculty of Health Science, University of Witwatersrand, 7 York Road, Parktown, Johannesburg 2193, South Africa.
المصدر: Viruses [Viruses] 2024 Jun 29; Vol. 16 (7). Date of Electronic Publication: 2024 Jun 29.
نوع المنشور: Journal Article; Comparative Study
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI
مواضيع طبية MeSH: Hepatitis B virus*/genetics , Proteomics* , Genotype*, Humans ; Cell Line, Tumor ; Signal Transduction ; Africa South of the Sahara ; Proteome ; rhoC GTP-Binding Protein/metabolism ; rhoC GTP-Binding Protein/genetics ; Carcinoma, Hepatocellular/virology ; Carcinoma, Hepatocellular/metabolism ; Carcinoma, Hepatocellular/genetics ; Transfection ; Liver Neoplasms/virology ; Liver Neoplasms/metabolism ; Liver Neoplasms/genetics ; Hepatitis B/virology ; Hepatitis B/metabolism ; Hepatitis B/genetics
مستخلص: In sub-Saharan Africa (SSA), the (sub)genotypes A1, D3, and E of the hepatitis B virus (HBV) prevail. Individuals infected with subgenotype A1 have a 4.5-fold increased risk of HCC compared to those infected with other (sub)genotypes. The effect of (sub)genotypes on protein expression and host signalling has not been studied. Mass spectrometry was used to analyse the proteome of Huh7 cells transfected with replication-competent clones. Proteomic analysis revealed significantly differentially expressed proteins between SSA (sub)genotypes. Different (sub)genotypes have the propensity to dysregulate specific host signalling pathways. Subgenotype A1 resulted in dysregulation within the Ras pathway. Ras-associated protein, RhoC, was significantly upregulated in cells transfected with subgenotype A1 compared to those transfected with other (sub)genotypes, on both a proteomic (>1.5-fold) and mRNA level ( p < 0.05). Two of the main cellular signalling pathways involving RHOC, MAPK and PI3K/Akt/mTOR, regulate cell growth, motility, and survival. Downstream signalling products of these pathways have been shown to increase MMP2 and MMP9 expression. An extracellular MMP2 and MMP9 ELISA revealed a non-significant increase in MMP2 and MMP9 in the cells transfected with A1 compared to the other (sub)genotypes ( p < 0.05). The upregulated Ras-associated proteins have been implicated as oncoproteins in various cancers and could contribute to the increased hepatocarcinogenic potential of A1.
References: J Virol. 1996 Sep;70(9):5845-51. (PMID: 8709203)
Semin Liver Dis. 2005;25 Suppl 1:3-8. (PMID: 16103976)
Gastroenterology. 2003 Nov;125(5):1370-8. (PMID: 14598253)
Gastroenterology. 1989 Jul;97(1):136-9. (PMID: 2470634)
Intervirology. 2014;57(3-4):141-50. (PMID: 25034481)
J Exp Clin Cancer Res. 2019 Jul 24;38(1):328. (PMID: 31340863)
Cell Microbiol. 2020 Feb;22(2):e13131. (PMID: 31746509)
J Gastroenterol. 2004 Sep;39(9):844-50. (PMID: 15565403)
Oncol Rep. 2016 Jun;35(6):3489-95. (PMID: 27108677)
PLoS One. 2013;8(1):e54493. (PMID: 23382905)
Hepatol Res. 2007 Jul;37(s1):S9-S19. (PMID: 17627641)
J Exp Clin Cancer Res. 2009 Apr 29;28:58. (PMID: 19402906)
J Viral Hepat. 2008 Jul;15(7):490-7. (PMID: 18221299)
Viruses. 2020 Mar 23;12(3):. (PMID: 32210021)
World J Gastroenterol. 2003 Sep;9(9):1950-3. (PMID: 12970882)
Exp Cell Res. 2004 May 1;295(2):300-14. (PMID: 15093731)
Microsc Res Tech. 2020 May;83(5):499-506. (PMID: 31926041)
Infect Drug Resist. 2021 Nov 09;14:4701-4715. (PMID: 34795487)
Nucleic Acids Res. 2017 Jan 4;45(D1):D183-D189. (PMID: 27899595)
Int J Infect Dis. 2014 Dec;29:125-32. (PMID: 25449246)
PLoS One. 2020 Oct 13;15(10):e0240453. (PMID: 33048956)
Hepatol Res. 2008 Jun;38(6):580-92. (PMID: 18201182)
J Virol. 1993 Nov;67(11):6507-12. (PMID: 7692081)
Cancer Treat Rev. 2013 Dec;39(8):935-46. (PMID: 23643661)
Front Microbiol. 2021 Nov 04;12:758613. (PMID: 34803982)
Nat Genet. 2012 May 06;44(6):694-8. (PMID: 22561517)
Liver Int. 2010 May;30(5):642-56. (PMID: 20726051)
Nat Protoc. 2013 Aug;8(8):1551-66. (PMID: 23868073)
CA Cancer J Clin. 2021 May;71(3):209-249. (PMID: 33538338)
Nat Genet. 2014 Dec;46(12):1267-73. (PMID: 25362482)
Lancet. 2018 Nov 24;392(10161):2313-2324. (PMID: 30496122)
Arch Virol. 2017 Oct;162(10):2937-2947. (PMID: 28685286)
PLoS One. 2010 Feb 19;5(2):e9297. (PMID: 20174575)
Liver Int. 2017 Aug;37(8):1193-1201. (PMID: 28111883)
Int J Cancer. 2021 Apr 5;:. (PMID: 33818764)
Hepatology. 2022 Feb;75(2):438-454. (PMID: 34580902)
Nature. 1991 Apr 4;350(6317):429-31. (PMID: 1672732)
J Virol. 2007 Sep;81(18):10072-80. (PMID: 17609269)
S Afr Med J. 2014 Jul 25;104(9):610-2. (PMID: 25212400)
Intervirology. 2016;59(1):48-59. (PMID: 27553619)
J Hepatol. 2017 Feb;66(2):355-362. (PMID: 27693539)
Microorganisms. 2023 Apr 22;11(5):. (PMID: 37317074)
J Virol. 1990 Jun;64(6):2819-24. (PMID: 2335817)
Dig Liver Dis. 2009 Dec;41(12):875-80. (PMID: 19473897)
Int J Mol Sci. 2019 Jan 30;20(3):. (PMID: 30704071)
J Hepatocell Carcinoma. 2021 May 20;8:435-450. (PMID: 34046368)
J Hepatol. 2014 Apr;60(4):855-65. (PMID: 24308993)
Intervirology. 2004;47(6):289-309. (PMID: 15564741)
Infect Genet Evol. 2013 Jun;16:355-61. (PMID: 23538336)
Cell Microbiol. 2017 May;19(5):. (PMID: 27886437)
Oncogene. 2005 Apr 18;24(17):2899-908. (PMID: 15838523)
Cell. 2019 Oct 3;179(2):561-577.e22. (PMID: 31585088)
Rev Med Virol. 2016 Jul;26(4):285-303. (PMID: 27139263)
Signal Transduct Target Ther. 2023 Sep 15;8(1):352. (PMID: 37709773)
J Hepatol. 2016 Apr;64(1 Suppl):S84-S101. (PMID: 27084040)
Antimicrob Agents Chemother. 2017 Jul 25;61(8):. (PMID: 28559272)
J Transl Med. 2014 Sep 19;12:234. (PMID: 25236186)
Ann Surg Oncol. 2012 Jul;19 Suppl 3:S375-84. (PMID: 21681378)
Mol Med Rep. 2016 Jun;13(6):5255-61. (PMID: 27108649)
Front Oncol. 2014 Apr 14;4:64. (PMID: 24782981)
Bioinformatics. 2020 Apr 15;36(8):2628-2629. (PMID: 31882993)
Fibrogenesis Tissue Repair. 2012 Jan 03;5(1):1. (PMID: 22214245)
Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50. (PMID: 16199517)
Virus Res. 2007 Aug;127(2):164-76. (PMID: 17383765)
Biochem J. 1996 Sep 1;318 ( Pt 2):717-22. (PMID: 8809067)
Nat Genet. 2015 May;47(5):505-511. (PMID: 25822088)
Lancet. 1989 Sep 9;2(8663):588-91. (PMID: 2570285)
J Gen Virol. 1992 Dec;73 ( Pt 12):3141-5. (PMID: 1469353)
World J Gastroenterol. 2014 Jun 21;20(23):7152-68. (PMID: 24966586)
Electrophoresis. 2003 Oct;24(19-20):3493-9. (PMID: 14595696)
J Biol Chem. 2008 Nov 21;283(47):32352-60. (PMID: 18805786)
S Afr Med J. 2018 Aug 08;108(8b):17-21. (PMID: 30182908)
Hepatology. 2006 Oct;44(4):915-24. (PMID: 17006908)
Cancer Cell Int. 2021 Oct 9;21(1):527. (PMID: 34627249)
Biomed Res Int. 2015;2015:821761. (PMID: 26380295)
Mol Cell. 2001 Jun;7(6):1165-76. (PMID: 11430820)
Exp Cell Res. 2004 Nov 15;301(1):43-9. (PMID: 15501444)
Viruses. 2022 Jan 03;14(1):. (PMID: 35062286)
Nature. 1991 Apr 4;350(6317):427-8. (PMID: 1849234)
Hepatol Res. 2013 Apr;43(4):355-64. (PMID: 22978460)
EMBO J. 2003 May 1;22(9):2104-16. (PMID: 12727877)
Vaccine. 2005 Mar 31;23(19):2409-23. (PMID: 15752827)
J Proteome Res. 2016 Feb 5;15(2):487-98. (PMID: 26709725)
J Gen Virol. 1988 Oct;69 ( Pt 10):2575-83. (PMID: 3171552)
J Virol. 2009 Oct;83(20):10538-47. (PMID: 19640977)
Nat Rev Dis Primers. 2021 Jan 21;7(1):6. (PMID: 33479224)
Front Immunol. 2020 Jul 09;11:1450. (PMID: 32733480)
Biomed Pharmacother. 2014 Oct;68(8):1023-9. (PMID: 25443414)
J Virol Methods. 2014 Jul;203:54-64. (PMID: 24681050)
J Med Virol. 2009 Sep;81(9):1525-30. (PMID: 19623667)
Hepatology. 2004 Sep;40(3):747-55. (PMID: 15349915)
J Neurosci. 2006 May 31;26(22):5996-6003. (PMID: 16738242)
Sci Rep. 2018 Jul 25;8(1):11185. (PMID: 30046100)
Int J Mol Sci. 2021 Sep 23;22(19):. (PMID: 34638601)
J Med Virol. 2008 Jan;80(1):27-46. (PMID: 18041043)
معلومات مُعتمدة: https://cansa.org.za/, accessed on 1 January 2023 Cancer Association of South Africa
فهرسة مساهمة: Keywords: (sub)genotypes; hepatitis B virus; hepatocellular carcinoma; oncogenic pathways; viral oncogenesis
المشرفين على المادة: 0 (Proteome)
EC 3.6.5.2 (RHOC protein, human)
EC 3.6.5.2 (rhoC GTP-Binding Protein)
تواريخ الأحداث: Date Created: 20240727 Date Completed: 20240728 Latest Revision: 20240729
رمز التحديث: 20240729
مُعرف محوري في PubMed: PMC11281506
DOI: 10.3390/v16071052
PMID: 39066215
قاعدة البيانات: MEDLINE
الوصف
تدمد:1999-4915
DOI:10.3390/v16071052