دورية أكاديمية
Modulation of the intestinal mucosal and cell-mediated response against natural helminth infection in the African catfish Clarias gariepinus.
العنوان: | Modulation of the intestinal mucosal and cell-mediated response against natural helminth infection in the African catfish Clarias gariepinus. |
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المؤلفون: | Abdel-Hakeem SS; Parasitology Laboratory, Zoology and Entomology Department, Faculty of Science, Assiut University, Assiut, 71526, Egypt. sara_assiut86@aun.edu.eg., Fadladdin YAJ; Faculty of Sciences, Department of Biological Sciences, King Abdulaziz University, Jeddah, Saudi Arabia., Khormi MA; Department of Biology, College of Science, Jazan University, Saudi Arabia, P.O. Box. 114, Jazan, 45142, Kingdom of Saudi Arabia., Abd-El-Hafeez HH; Department of Cell and Tissues, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt. hhnnzz91@aun.edu.eg. |
المصدر: | BMC veterinary research [BMC Vet Res] 2024 Jul 27; Vol. 20 (1), pp. 335. Date of Electronic Publication: 2024 Jul 27. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101249759 Publication Model: Electronic Cited Medium: Internet ISSN: 1746-6148 (Electronic) Linking ISSN: 17466148 NLM ISO Abbreviation: BMC Vet Res Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: London : BioMed Central, 2005- |
مواضيع طبية MeSH: | Catfishes*/parasitology , Fish Diseases*/parasitology , Fish Diseases*/immunology , Intestinal Mucosa*/parasitology , Intestinal Mucosa*/immunology , Helminthiasis, Animal*/parasitology, Animals ; Immunity, Cellular ; Vascular Endothelial Growth Factor A/metabolism ; Proliferating Cell Nuclear Antigen/metabolism ; Antigens, Differentiation, Myelomonocytic/metabolism ; Intestinal Diseases, Parasitic/veterinary ; Intestinal Diseases, Parasitic/parasitology ; Intestinal Diseases, Parasitic/immunology ; Antigens, CD/metabolism |
مستخلص: | Fish gut is a versatile organ serving as the primary pathway for invasion by pathogens, particularly parasites, playing a crucial role in modulating the intestinal adaptive immune response. This study aimed to investigate the cellular-mediated reaction, mucosal acidity, and the expression of proliferating cell nuclear antigen (PCNA), vascular endothelial growth factor (VEGF), and CD68 in the intestines of catfish, Clarias gariepinus, naturally infected with helminths. Forty catfish were collected from the Nile River and examined for intestinal parasites. The intestinal tissues of the control and infected fish were fixed for histochemical and immunohistochemical studies. Two groups of helminths were found: cestodes Tetracampos ciliotheca and Polyonchobothrium clarias, and nematodes Paracamallanus cyathopharynx, with a prevalence rate of 63.63%, 18.0%, and 18.0%, respectively. Our results showed that the infected fish had a statistically significant rise in the activity of immune cells, including mast cells, eosinophil granular cells, and dendritic cells. This correlated with upregulation in the expressions of PCNA, VEGF, and CD68. Histochemical analyses demonstrated a marked increase in acidic mucus production, Sudan black B, and bromophenol mercury blue. This study enriches our understanding of the evolution of vertebrate immunity in combating intestinal parasitic infections and the host's adaptive responses. (© 2024. The Author(s).) |
References: | J Therm Biol. 2016 Apr;57:21-34. (PMID: 27033036) Biotech Histochem. 1994 Nov;69(6):317-23. (PMID: 7703303) Fish Shellfish Immunol. 2017 May;64:243-250. (PMID: 28330806) Fish Shellfish Immunol. 2018 Apr;75:172-180. (PMID: 29432864) Molecules. 2022 Aug 31;27(17):. (PMID: 36080365) Immunity. 2001 May;14(5):495-8. (PMID: 11371351) J Cell Biol. 1996 Oct;135(1):279-90. (PMID: 8858180) J Exp Med. 1998 Sep 21;188(6):1135-45. (PMID: 9743532) Front Immunol. 2015 Jul 15;6:354. (PMID: 26236311) Curr Top Microbiol Immunol. 2000;248:249-70. (PMID: 10793481) Microb Pathog. 2020 Oct;147:104404. (PMID: 32781103) Nature. 2008 Mar 27;452(7186):423-8. (PMID: 18344981) Microsc Res Tech. 2023 Sep;86(9):1108-1121. (PMID: 37337938) Microsc Microanal. 2019 Dec;25(6):1498-1508. (PMID: 31718724) Mar Pollut Bull. 2009 Jan;58(1):7-10. (PMID: 18929373) Microsc Microanal. 2024 Apr 29;30(2):368-381. (PMID: 38323506) Biology (Basel). 2015 Aug 12;4(3):525-39. (PMID: 26274978) Immunol Lett. 2012 Nov-Dec;148(1):23-33. (PMID: 22902399) Int J Clin Exp Pathol. 2015 Nov 01;8(11):13945-56. (PMID: 26823706) Parasit Vectors. 2012 Sep 11;5:198. (PMID: 22967751) Cell. 2006 Feb 24;124(4):815-22. (PMID: 16497590) Histol Histopathol. 2006 Jun;21(6):579-93. (PMID: 16528668) Saudi J Biol Sci. 2022 Aug;29(8):103366. (PMID: 35860497) Microsc Microanal. 2023 Apr 5;29(2):739-753. (PMID: 37749745) PLoS One. 2016 Mar 15;11(3):e0151396. (PMID: 26978773) Curr Opin Immunol. 2012 Aug;24(4):459-66. (PMID: 22795966) J Histochem Cytochem. 1981 Apr;29(4):577-80. (PMID: 6166661) Clin Dev Immunol. 2013;2013:536534. (PMID: 24324508) J Vet Diagn Invest. 1994 Oct;6(4):458-65. (PMID: 7532014) Microsc Res Tech. 2022 Nov;85(11):3565-3581. (PMID: 35876377) Mar Pollut Bull. 2014 Jan 15;78(1-2):235-45. (PMID: 24239097) Nature. 1984 May 24-30;309(5966):374-6. (PMID: 6145097) Semin Immunopathol. 2017 Feb;39(2):199-213. (PMID: 27587063) Virchows Arch. 2000 Mar;436(3):243-8. (PMID: 10782883) Arch Pathol Lab Med. 2008 Mar;132(3):476-89. (PMID: 18318588) Microsc Res Tech. 2022 Nov;85(11):3653-3663. (PMID: 35920019) Fish Shellfish Immunol. 2006 Feb;20(2):192-208. (PMID: 15978838) Lab Invest. 1997 Feb;76(2):245-55. (PMID: 9042161) Allergy Asthma Immunol Res. 2012 Nov;4(6):332-40. (PMID: 23115729) Life Sci. 2022 Nov 1;308:120954. (PMID: 36103960) Fish Shellfish Immunol. 2007 Mar;22(3):218-29. (PMID: 16824768) Physiol Rev. 1997 Oct;77(4):1033-79. (PMID: 9354811) Microsc Res Tech. 2022 Feb;85(2):799-806. (PMID: 34590388) Semin Immunopathol. 2021 Jun;43(3):295-306. (PMID: 33929602) Nature. 1992 Oct 29;359(6398):843-5. (PMID: 1279431) Dev Comp Immunol. 2016 Nov;64:178-86. (PMID: 26872545) Science. 2012 Feb 24;335(6071):936-41. (PMID: 22363001) J Exp Med. 1947 Jul 31;86(2):107-16. (PMID: 19871658) Pol J Pathol. 2012 Mar;63(1):1-7. (PMID: 22535614) J Hematol Oncol. 2013 Aug 02;6:56. (PMID: 23915951) |
فهرسة مساهمة: | Keywords: CD68; Catfish; Histochemical; Intestinal immune cells; PCNA; VEGF |
المشرفين على المادة: | 0 (Vascular Endothelial Growth Factor A) 0 (Proliferating Cell Nuclear Antigen) 0 (Antigens, Differentiation, Myelomonocytic) 0 (Antigens, CD) |
تواريخ الأحداث: | Date Created: 20240727 Date Completed: 20240728 Latest Revision: 20240730 |
رمز التحديث: | 20240730 |
مُعرف محوري في PubMed: | PMC11282724 |
DOI: | 10.1186/s12917-024-04153-1 |
PMID: | 39068442 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1746-6148 |
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DOI: | 10.1186/s12917-024-04153-1 |