دورية أكاديمية
Hydrogel-based hybrid membrane enhances in vitro ophthalmic drug evaluation in the OphthalMimic device.
| العنوان: | Hydrogel-based hybrid membrane enhances in vitro ophthalmic drug evaluation in the OphthalMimic device. |
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| المؤلفون: | Barbalho GN; Laboratory of Food, Drugs, and Cosmetics (LTMAC), University of Brasilia, 70910-900 Brasília, DF, Brazil., Falcão MA; Laboratory of Food, Drugs, and Cosmetics (LTMAC), University of Brasilia, 70910-900 Brasília, DF, Brazil., Amaral VA; Laboratory of Food, Drugs, and Cosmetics (LTMAC), University of Brasilia, 70910-900 Brasília, DF, Brazil., Contarato JL; Laboratory of Food, Drugs, and Cosmetics (LTMAC), University of Brasilia, 70910-900 Brasília, DF, Brazil., Gelfuso GM; Laboratory of Food, Drugs, and Cosmetics (LTMAC), University of Brasilia, 70910-900 Brasília, DF, Brazil., Cunha-Filho M; Laboratory of Food, Drugs, and Cosmetics (LTMAC), University of Brasilia, 70910-900 Brasília, DF, Brazil., Gratieri T; Laboratory of Food, Drugs, and Cosmetics (LTMAC), University of Brasilia, 70910-900 Brasília, DF, Brazil. Electronic address: tgratieri@unb.br. |
| المصدر: | Methods (San Diego, Calif.) [Methods] 2024 Jul 27; Vol. 230, pp. 21-31. Date of Electronic Publication: 2024 Jul 27. |
| Publication Model: | Ahead of Print |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 9426302 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9130 (Electronic) Linking ISSN: 10462023 NLM ISO Abbreviation: Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Duluth, MN : Academic Press Original Publication: San Diego : Academic Press, c1990- |
| مستخلص: | Envisaging to improve the evaluation of ophthalmic drug products while minimizing the need for animal testing, our group developed the OphthalMimic device, a 3D-printed device that incorporates an artificial lacrimal flow, a cul-de-sac area, a moving eyelid, and a surface that interacts effectively with ophthalmic formulations, thereby providing a close representation of human ocular conditions. An important application of such a device would be its use as a platform for dissolution/release tests that closely mimic in vivo conditions. However, the surface that artificially simulates the cornea should have a higher resistance (10 min) than the previously described polymeric films (5 min). For this key assay upgrade, we describe the process of obtaining and thoroughly characterizing a hydrogel-based hybrid membrane to be used as a platform base to simulate the cornea artificially. Also, the OphthalMimic device suffered design improvements to fit the new membrane and incorporate the moving eyelid. The results confirmed the successful synthesis of the hydrogel components. The membrane's water content (86.25 ± 0.35 %) closely mirrored the human cornea (72 to 85 %). Furthermore, morphological analysis supported the membrane's comparability to the natural cornea. Finally, the performance of different formulations was analysed, demonstrating that the device could differentiate their drainage profile through the viscosity of PLX 14 (79 ± 5 %), PLX 16 (72 ± 4 %), and PLX 20 (57 ± 14 %), and mucoadhesion of PLXCS0.5 (69 ± 1 %), PLX16CS1.0 (65 ± 3 %), PLX16CS1.25 (67 ± 3 %), and the solution (97 ± 8 %). In conclusion, using the hydrogel-based hybrid membrane in the OphthalMimic device represents a significant advancement in the field of ophthalmic drug evaluation, providing a valuable platform for dissolution/release tests. Such a platform aligns with the ethical mandate to reduce animal testing and promises to accelerate the development of safer and more effective ophthalmic drugs. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: 3D-printed device; Corneal model; Hydrogel-based hybrid membrane; In vitro model; Ocular delivery; Retention time |
| تواريخ الأحداث: | Date Created: 20240729 Latest Revision: 20240801 |
| رمز التحديث: | 20240802 |
| DOI: | 10.1016/j.ymeth.2024.07.010 |
| PMID: | 39074539 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1095-9130 |
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| DOI: | 10.1016/j.ymeth.2024.07.010 |