دورية أكاديمية
Biocompatibility and customizability: Expanding possibilities with 3D printed guide cannulas.
| العنوان: | Biocompatibility and customizability: Expanding possibilities with 3D printed guide cannulas. |
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| المؤلفون: | Kazanskaya RB; Research Center of Neurology, Volokolamskoye Shosse 80, Moscow 125367, Russia; Biological Department, Saint Petersburg State University, Universitetskaya Emb. 7/9, St. Petersburg 199034, Russia. Electronic address: st059046@student.spbu.ru., Berliand AN; Research Center of Neurology, Volokolamskoye Shosse 80, Moscow 125367, Russia; NUST MISIS, Leninskiy Prospekt 4, Moscow 119049, Russia., Volnova AB; Biological Department, Saint Petersburg State University, Universitetskaya Emb. 7/9, St. Petersburg 199034, Russia; Institute of Translational Biomedicine, Saint Petersburg State University, Universitetskaya Emb. 7/9, St. Petersburg 199034, Russia., Lopachev AV; Research Center of Neurology, Volokolamskoye Shosse 80, Moscow 125367, Russia; Institute of Translational Biomedicine, Saint Petersburg State University, Universitetskaya Emb. 7/9, St. Petersburg 199034, Russia. |
| المصدر: | Journal of neuroscience methods [J Neurosci Methods] 2024 Oct; Vol. 410, pp. 110237. Date of Electronic Publication: 2024 Jul 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 7905558 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-678X (Electronic) Linking ISSN: 01650270 NLM ISO Abbreviation: J Neurosci Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press. |
| مواضيع طبية MeSH: | Printing, Three-Dimensional*/instrumentation , Cannula* , Mice, Inbred C57BL*, Animals ; Neurons/physiology ; Rats ; Mice ; Biocompatible Materials ; Cells, Cultured ; Equipment Design ; Plastics |
| مستخلص: | Background: Intracerebral cannulation bypasses the blood-brain barrier, and is frequently used for targeted drug delivery to specific brain structures. Despite the availability of brain infusion kits and manual injections without cannulation, the traditional design of guide cannulas continues to be utilized in research. Several protocols describing guide cannula manufacture from stainless steel needles have been published previously. New Method: We describe a method for producing the first fully plastic guide cannula intended for intracerebroventricular injections in mice using Dental Sand A1-A2 resin and digital light processing 3D printing. Results: The lack of resin neurotoxicity for primary rat cortical neuron cultures was shown. Histological evaluations performed 6 weeks after guide cannula implantation to C57/black mice show that plastic cannula are biocompatible. Microglial and astroglial reactions to plastic cannulas are reduced compared to lab-made stainless steel cannulas. Plastic cannulas are less prone to obstruction, and remained unobstructed over the course of 3 weeks of daily injections, while 50 % of stainless steel cannula became impassable by the 2 week mark. Comparison With Existing Methods: These are the first published cannulas intended for applications in mice which combine the presence of usable threads, allowing dummy cannula fixation, with a low profile and small footprint compared to commercially available cannulas. Conclusions: Editable parametric and stl files for reproducing the cannulas presented in this manuscript are included. The method described in this paper is accessible to most laboratories, enabling near-perfect standardization in length combined with a high level of customizability. Competing Interests: Declaration of Competing Interest None Conflict of Interest Authors report no conflict of interest (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Additive manufacturing; Biocompatibility; Drug delivery; Guide cannulas; Intracerebroventricular administration |
| المشرفين على المادة: | 0 (Biocompatible Materials) 0 (Plastics) |
| تواريخ الأحداث: | Date Created: 20240730 Date Completed: 20240823 Latest Revision: 20240823 |
| رمز التحديث: | 20240826 |
| DOI: | 10.1016/j.jneumeth.2024.110237 |
| PMID: | 39079571 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-678X |
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| DOI: | 10.1016/j.jneumeth.2024.110237 |