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The photoreactivation of 6 - 4 photoproducts in chloroplast and nuclear DNA depends on the amount of the Arabidopsis UV repair defective 3 protein.

التفاصيل البيبلوغرافية
العنوان: The photoreactivation of 6 - 4 photoproducts in chloroplast and nuclear DNA depends on the amount of the Arabidopsis UV repair defective 3 protein.
المؤلفون: Zgłobicki P; Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, Kraków, 30-387, Poland., Hermanowicz P; Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7A, Kraków, 30-387, Poland., Kłodawska K; Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, Kraków, 30-387, Poland., Bażant A; Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, Kraków, 30-387, Poland., Łabuz J; Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7A, Kraków, 30-387, Poland., Grzyb J; Department of Biophysics, Faculty of Biotechnology, University of Wrocław, F. Joliot-Curie 14a, Wrocław, 50-383, Poland., Dutka M; Department of Molecular Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, Kraków, 30-387, Poland., Kowalska E; Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, Kraków, 30-387, Poland., Jawor J; Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, Kraków, 30-387, Poland., Leja K; Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, Kraków, 30-387, Poland.; Doctoral School of Exact and Natural Sciences, Jagiellonian University, prof. S. Łojasiewicza 11, Kraków, 30-348, Poland., Banaś AK; Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, Kraków, 30-387, Poland. a_katarzyna.banas@uj.edu.pl.
المصدر: BMC plant biology [BMC Plant Biol] 2024 Jul 30; Vol. 24 (1), pp. 723. Date of Electronic Publication: 2024 Jul 30.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 100967807 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2229 (Electronic) Linking ISSN: 14712229 NLM ISO Abbreviation: BMC Plant Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2001-
مواضيع طبية MeSH: Arabidopsis*/genetics , Arabidopsis*/radiation effects , Arabidopsis*/metabolism , Arabidopsis Proteins*/metabolism , Arabidopsis Proteins*/genetics , Cell Nucleus*/metabolism , Cell Nucleus*/radiation effects , Deoxyribodipyrimidine Photo-Lyase*/metabolism , Deoxyribodipyrimidine Photo-Lyase*/genetics , Plants, Genetically Modified* , DNA Repair*, Ultraviolet Rays ; DNA, Plant/metabolism ; DNA, Plant/genetics ; Pyrimidine Dimers/metabolism ; Pyrimidine Dimers/genetics ; DNA, Chloroplast/genetics ; DNA, Chloroplast/metabolism ; Chloroplasts/metabolism ; DNA Damage
مستخلص: Background: 6 - 4 photoproducts are the second most common UV-induced DNA lesions after cyclobutane pyrimidine dimers. In plants, they are mainly repaired by photolyases in a process called photoreactivation. While pyrimidine dimers can be deleterious, leading to mutagenesis or even cell death, 6 - 4 photoproducts can activate specific signaling pathways. Therefore, their removal is particularly important, especially for plants exposed to high UV intensities due to their sessile nature. Although photoreactivation in nuclear DNA is well-known, its role in plant organelles remains unclear. In this paper we analyzed the activity and localization of GFP-tagged AtUVR3, the 6 - 4 photoproduct specific photolyase.
Results: Using transgenic Arabidopsis with different expression levels of AtUVR3, we confirmed a positive trend between these levels and the rate of 6 - 4 photoproduct removal under blue light. Measurements of 6 - 4 photoproduct levels in chloroplast and nuclear DNA of wild type, photolyase mutants, and transgenic plants overexpressing AtUVR3 showed that the photoreactivation is the main repair pathway responsible for the removal of these lesions in both organelles. The GFP-tagged AtUVR3 was predominantly located in nuclei with a small fraction present in chloroplasts and mitochondria of transgenic Arabidopsis thaliana and Nicotiana tabacum lines. In chloroplasts, this photolyase co-localized with the nucleoid marked by plastid envelope DNA binding protein.
Conclusions: Photolyases are mainly localized in plant nuclei, with only a small fraction present in chloroplasts and mitochondria. Despite this unbalanced distribution, photoreactivation is the primary mechanism responsible for the removal of 6 - 4 photoproducts from nuclear and chloroplast DNA in adult leaves. The amount of the AtUVR3 photolyase is the limiting factor influencing the photoreactivation rate of 6 - 4 photoproducts. The efficient photoreactivation of 6 - 4 photoproducts in 35S: AtUVR3-GFP Arabidopsis and Nicotiana tabacum is a promising starting point to evaluate whether transgenic crops overproducing this photolyase are more tolerant to high UV irradiation and how they respond to other abiotic and biotic stresses under field conditions.
(© 2024. The Author(s).)
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معلومات مُعتمدة: 2016/22/E/NZ3/00326 National Science Centre, Poland
فهرسة مساهمة: Keywords: (6 − 4) pyrimidine–pyrimidone photoproduct; Arabidopsis; AtUVR3; Chloroplast nucleoid; Photolyase; Photoreactivation
المشرفين على المادة: 0 (Arabidopsis Proteins)
EC 4.1.99.3 (Deoxyribodipyrimidine Photo-Lyase)
0 (DNA, Plant)
0 (Pyrimidine Dimers)
0 (DNA, Chloroplast)
تواريخ الأحداث: Date Created: 20240730 Date Completed: 20240731 Latest Revision: 20240909
رمز التحديث: 20240909
مُعرف محوري في PubMed: PMC11287969
DOI: 10.1186/s12870-024-05439-0
PMID: 39080534
قاعدة البيانات: MEDLINE
الوصف
تدمد:1471-2229
DOI:10.1186/s12870-024-05439-0