دورية أكاديمية
Elucidating the multifaceted role of MGAT1 in hepatocellular carcinoma: integrative single-cell and spatial transcriptomics reveal novel therapeutic insights.
| العنوان: | Elucidating the multifaceted role of MGAT1 in hepatocellular carcinoma: integrative single-cell and spatial transcriptomics reveal novel therapeutic insights. |
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| المؤلفون: | Li Y; Department of General Medicine, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital, Taiyuan, China., Chen Y; Department of General Medicine, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital, Taiyuan, China.; Department of Geriatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China., Wang D; Department of General Medicine, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital, Taiyuan, China., Wu L; Tumor Center, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital, Taiyuan, China., Li T; Department of General Medicine, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital, Taiyuan, China., An N; Department of General Medicine, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital, Taiyuan, China., Yang H; The Gastroenterology Department, Shanxi Provincial People Hospital, Taiyuan, China. |
| المصدر: | Frontiers in immunology [Front Immunol] 2024 Jul 16; Vol. 15, pp. 1442722. Date of Electronic Publication: 2024 Jul 16 (Print Publication: 2024). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Lausanne : Frontiers Research Foundation] |
| مواضيع طبية MeSH: | Carcinoma, Hepatocellular*/genetics , Carcinoma, Hepatocellular*/therapy , Carcinoma, Hepatocellular*/immunology , Gene Expression Regulation, Neoplastic* , Liver Neoplasms*/genetics , Liver Neoplasms*/therapy , Liver Neoplasms*/immunology , Single-Cell Analysis* , Transcriptome* , Tumor Microenvironment*/genetics , Tumor Microenvironment*/immunology , N-Acetylglucosaminyltransferases*/genetics , N-Acetylglucosaminyltransferases*/metabolism, Animals ; Humans ; Biomarkers, Tumor/genetics ; Cell Line, Tumor ; Gene Expression Profiling ; Glycosyltransferases/genetics ; Immunotherapy/methods ; Macrophages/immunology ; Macrophages/metabolism |
| مستخلص: | Background: Glycosyltransferase-associated genes play a crucial role in hepatocellular carcinoma (HCC) pathogenesis. This study investigates their impact on the tumor microenvironment and molecular mechanisms, offering insights into innovative immunotherapeutic strategies for HCC. Methods: We utilized cutting-edge single-cell and spatial transcriptomics to examine HCC heterogeneity. Four single-cell scoring techniques were employed to evaluate glycosyltransferase genes. Spatial transcriptomic findings were validated, and bulk RNA-seq analysis was conducted to identify prognostic glycosyltransferase-related genes and potential immunotherapeutic targets. MGAT1's role was further explored through various functional assays. Results: Our analysis revealed diverse cell subpopulations in HCC with distinct glycosyltransferase gene activities, particularly in macrophages. Key glycosyltransferase genes specific to macrophages were identified. Temporal analysis illustrated macrophage evolution during tumor progression, while spatial transcriptomics highlighted reduced expression of these genes in core tumor macrophages. Integrating scRNA-seq, bulk RNA-seq, and spatial transcriptomics, MGAT1 emerged as a promising therapeutic target, showing significant potential in HCC immunotherapy. Conclusion: This comprehensive study delves into glycosyltransferase-associated genes in HCC, elucidating their critical roles in cellular dynamics and immune cell interactions. Our findings open new avenues for immunotherapeutic interventions and personalized HCC management, pushing the boundaries of HCC immunotherapy. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Li, Chen, Wang, Wu, Li, An and Yang.) |
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| فهرسة مساهمة: | Keywords: hepatocellular carcinoma; multi-omics data; novel biomarkers; pathogenesis; single-cell RNA sequencing; spatial transcriptomics; tumor heterogeneity; tumor microenvironment |
| المشرفين على المادة: | 0 (Biomarkers, Tumor) EC 2.4.- (Glycosyltransferases) EC 2.4.1.- (MGAT1 protein, human) EC 2.4.1.- (N-Acetylglucosaminyltransferases) |
| تواريخ الأحداث: | Date Created: 20240731 Date Completed: 20240731 Latest Revision: 20240801 |
| رمز التحديث: | 20240801 |
| مُعرف محوري في PubMed: | PMC11286416 |
| DOI: | 10.3389/fimmu.2024.1442722 |
| PMID: | 39081317 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-3224 |
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| DOI: | 10.3389/fimmu.2024.1442722 |