دورية أكاديمية
Pigment Dispersion Contributes to Ocular Immune Privilege in a DBA/2J Mouse Model of Pigmentary Glaucoma.
| العنوان: | Pigment Dispersion Contributes to Ocular Immune Privilege in a DBA/2J Mouse Model of Pigmentary Glaucoma. |
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| المؤلفون: | Li Q; Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical, University, Beijing Ophthalmology and Visual Sciences Key Lab, Beijing, P. R. China., Pu L; Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical, University, Beijing Ophthalmology and Visual Sciences Key Lab, Beijing, P. R. China., Cheng S; Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical, University, Beijing Ophthalmology and Visual Sciences Key Lab, Beijing, P. R. China., Tang S; Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical, University, Beijing Ophthalmology and Visual Sciences Key Lab, Beijing, P. R. China., Zhang J; Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical, University, Beijing Ophthalmology and Visual Sciences Key Lab, Beijing, P. R. China., Qing G; Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical, University, Beijing Ophthalmology and Visual Sciences Key Lab, Beijing, P. R. China. |
| المصدر: | Investigative ophthalmology & visual science [Invest Ophthalmol Vis Sci] 2024 Jul 01; Vol. 65 (8), pp. 51. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Association For Research In Vision And Ophthalmology (Arvo) Country of Publication: United States NLM ID: 7703701 Publication Model: Print Cited Medium: Internet ISSN: 1552-5783 (Electronic) Linking ISSN: 01460404 NLM ISO Abbreviation: Invest Ophthalmol Vis Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Brookline Ma : Association For Research In Vision And Ophthalmology (Arvo) Original Publication: St. Louis, Mosby. |
| مواضيع طبية MeSH: | Mice, Inbred DBA* , Aqueous Humor*/metabolism , Aqueous Humor*/immunology , Disease Models, Animal* , Mice, Inbred C57BL* , T-Lymphocytes, Regulatory*/immunology , Glaucoma, Open-Angle*/immunology , Immune Privilege*, Animals ; Mice ; Anterior Chamber/immunology ; Transforming Growth Factor beta2 ; Intraocular Pressure/physiology ; Enzyme-Linked Immunosorbent Assay ; Flow Cytometry ; Interleukin-10 ; Hypersensitivity, Delayed/immunology ; Interferon-gamma/metabolism ; Immunohistochemistry ; Female |
| مستخلص: | Purpose: To investigate the effects of anterior chamber pigment dispersion on ocular immune privilege and the possible mechanisms involved in a DBA/2J mouse model of pigmentary glaucoma. Methods: DBA/2J mice were utilized as a pigment dispersion model, and age-matched C57BL/6J mice were used as the control group in this study. Proteins in the aqueous humor (AH) and serum were quantified using the bicinchoninic acid assay. Immune cells in the AH were detected using hematoxylin and eosin staining and immunocytochemistry. The expression of TGF-β2 in the AH and cytokine levels (IL-10, IFN-γ) in serum were measured using ELISA. Anterior chamber-associated immune deviation (ACAID) was induced in DBA/2J mice by injecting antigens into the anterior chamber. Delayed-type hypersensitivity (DTH) assays were used to assess the induction of ACAID. In DBA/2J mice, before and after pigment dispersion, following anterior chamber injection of pigment particles, and after ACAID modeling, the expression of regulatory T cells (Tregs) was detected using flow cytometry. Results: Compared to C57BL/6J mice, the protein concentration, immune cell count, and TGF-β2 levels in the AH were elevated in DBA/2J mice. Protein concentration and IL-10 levels in serum were increased, while IFN-γ levels were decreased in DBA/2J. Additionally, the expression of Treg cells in the spleen of DBA/2J mice was significantly increased after pigment dispersion and anterior chamber injection of pigment particles. At 3 and 6 months, DTH responses in DBA/2J mice were not inhibited, thus preventing ACAID induction. However, the opposite was observed at 9 months in DBA/2J mice. Furthermore, the ACAID group exhibited an augmented expression of Treg cells. Conclusions: Dispersion of pigment particles in the anterior chamber of the eye enhances the state of ocular immune privilege by influencing the immunosuppressive microenvironment and inducing more Treg cells to reestablish ACAID. |
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| المشرفين على المادة: | 0 (Transforming Growth Factor beta2) 130068-27-8 (Interleukin-10) 82115-62-6 (Interferon-gamma) |
| SCR Disease Name: | Glaucoma-Related Pigment Dispersion Syndrome |
| تواريخ الأحداث: | Date Created: 20240731 Date Completed: 20240731 Latest Revision: 20240802 |
| رمز التحديث: | 20240802 |
| مُعرف محوري في PubMed: | PMC11290564 |
| DOI: | 10.1167/iovs.65.8.51 |
| PMID: | 39083309 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1552-5783 |
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| DOI: | 10.1167/iovs.65.8.51 |