دورية أكاديمية
أعرض في EDS

Inopinatus corneliae sp. nov. gen. nov. isolated from human skin: A newly discovered keratinophilic hyphomycete, order Onygenales.

التفاصيل البيبلوغرافية
العنوان: Inopinatus corneliae sp. nov. gen. nov. isolated from human skin: A newly discovered keratinophilic hyphomycete, order Onygenales.
المؤلفون: Brasch J; Department of Dermatology, University Hospitals of Schleswig-Holstein, Campus Kiel, Kiel, Germany., Gräser Y; Institute of Microbiology, Infectious Diseases and Immunology, Charité, Berlin, Germany., Voss K; Department of Dermatology, University Hospitals of Schleswig-Holstein, Campus Kiel, Kiel, Germany., Langen KA; Department of Dermatology, University Hospitals of Schleswig-Holstein, Campus Kiel, Kiel, Germany., Yurkov A; Leibniz Institute DSMZ, German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany.
المصدر: Mycoses [Mycoses] 2024 Aug; Vol. 67 (8), pp. e13774.
نوع المنشور: Journal Article; Case Reports
اللغة: English
بيانات الدورية: Publisher: Grosse Verlag Country of Publication: Germany NLM ID: 8805008 Publication Model: Print Cited Medium: Internet ISSN: 1439-0507 (Electronic) Linking ISSN: 09337407 NLM ISO Abbreviation: Mycoses Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Berlin : Grosse Verlag, [1988?-
مواضيع طبية MeSH: Phylogeny* , Skin*/microbiology , Onygenales*/genetics , Onygenales*/classification , Onygenales*/isolation & purification , DNA, Fungal*/genetics , DNA, Ribosomal Spacer*/genetics , Sequence Analysis, DNA*, Humans ; Dermatomycoses/microbiology ; Keratins/metabolism ; DNA, Ribosomal/genetics ; Male ; Mycological Typing Techniques
مستخلص: Background: Fungi clinically relevant to human skin comprise prevalent commensals and well-known pathogens. Only rarely human skin harbours fungi that evade identification.
Objective: To characterise an enigmatic specimen isolated from a skin lesion.
Methods: A comprehensive clinical and mycological workup including conventional methods for phenotypic characterisation and sequencing based on internal transcribed spacer (ITS) and large subunit (LSU) regions to infer a phylogenetic tree.
Results: Cultures on common solid media were macroscopically inconspicuous initially until mycelial tufts developed on the surface, notably on potato dextrose agar. Polymorphous chlamydospores were detected but no aleurospores and ascomata. At 26°C, the isolate grew on standard agars, plant materials and garden soil and utilised peptone, keratins, lipids, inulin, erythrocytes and cellulose. It also grew at 5°C and at 37°C. Nucleotide sequences of its ITS region showed 93% similarity to sequences of different Malbranchea species. The closest matches among LSU rRNA sequences were obtained with the genera Amauroascus, Arthroderma, Auxarthronopsis and Malbranchea (93%-95%). A combined phylogenetic analysis placed the fungus in a sister clade to Neogymnomycetaceae, classified as incertae sedis in Onygenales, on a large distance to either Diploospora rosea or 'Amauroascus' aureus.
Conclusions: The genus Inopinatus gen. nov. (MB854685) with the species Inopinatus corneliae sp. nov. (MB854687) is introduced to accommodate our isolate (holotype: DSM 116806; isotypes: CBS 151104, IHEM 29063). Probably Inopinatus corneliae is a geophilic species that, although potentially harmful, was no relevant pathogen in our case. Its ecology, epidemiology and pathogenicity need to be further clarified.
(© 2024 The Author(s). Mycoses published by Wiley‐VCH GmbH.)
References: Campbell CK, Johnson EM, Philpot CM, Warnock DW, eds. Identification of Pathogenic Fungi. Public Health Laboratory Service 1996.
Summerbell R, Kane J. Physiological and other special tests for identifying dermatophytes. In: Kane J, Summerbell R, Sigler L, Krajden S, Land G, eds. Laboratory Handbook of Dermatophytes. Star Publishing Company; 1997:45‐79.
Simpanya MF. Dermatophytes: their taxonomy, ecology and pathogenicity. In: Kushwaha RKS, Guarro J, eds. Biology of Dermatophytes and Other Keratinophilic Fungi. Bilbao: Revista Iberoamerican de Micología. Bilbao; 2000:1‐12.
de Hoog GS, Guarro J, Gené J, Figueras MJ. Atlas of clinical fungi. Web version July 10. Centraalbureau voor Schimmelcultures, Universitat Rovira i Vigili, Reus, Spain. 2024.
Brasch J. Dermatophytenspezies. “Neue” Taxonomie und “neue” Taxa. Hautarzt. 2008;59:971‐979.
Brasch J, Beck‐Jendroschek V, Voss K, Yurkov A, Gräser Y. Arthroderma chiloniense sp. nov. isolated from human stratum corneum: description of a new Arthroderma species. Mycoses. 2019;62:73‐80.
Brasch J, Beck‐Jendroschek V, Iturrieta‐González I, Voss K, Gené J. A human subcutaneous infection by Microascus ennothomasiorum sp. nov. Mycoses. 2019;62:157‐164.
Takashio M. Sexual reproduction of some Arthroderma and Nannizzia on diluted Sabouraud agar with or without salts. Mykosen. 1972;15:11‐17.
Ajello L, Georg LK. In vitro hair cultures for differentiating between atypical isolates of Trichophyton mentagrophytes and Trichophyton rubrum. Mycopathologia. 1957;8:3‐17.
Brasch J, Beck‐Jendroscheck V, Voss K, Yurkov A, Stchigel AM, Gräser Y. Xanthothecium peruvianum isolated from human stratum corneum: a case report, characterisation and short review that suggest emendation of Arachnomyces peruvianus. Mycoses. 2017;60:469‐476.
Slifkin M, Cumbie R. Evaluation of the tween opacity test for the identification of dermatophytes. Med Microbiol Lett. 1996;5:401‐407.
Siopi M, Efstathiou I, Arendrup MC, Meletiadis J. Development of an agar‐based screening method for terbinafine, itraconazole, and amorolfine susceptibility testing of Trichophyton spp. J Clin Microbiol. 2024;62(1):e0130823. doi:10.1128/jcm.01308–23.
de Hoog GS, Gerrits van den Ende AH. Molecular diagnostics of clinical strains of filamentous basidiomycetes. Mycoses. 1998;41:183‐189.
White TJ, Bruns T, Lee SJWT, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, eds. PCR Protocols: A Guide to Methods and Applications. Academic Press; 1990:315‐322.
Gardes M, Bruns TD. ITS primers with enhanced specificity for basidiomycetes‐application to the identification of mycorrhizae and rusts. Mol Ecol. 1993;2:113‐118.
Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several cryptococcus species. J Bacteriol. 1990;172:4238‐4246.
Kurtzman CP, Robnett CJ. Identification of clinically important ascomycetous yeasts based on nucleotide divergence in the 5'end of the large‐subunit (26S) ribosomal DNA gene. J Clin Microbiol. 1997;35:1216‐1223.
Kandemir H, Dukik K, de Melo TM, et al. Phylogenetic and ecological reevaluation of the order Onygenales. Fungal Divers. 2022;115:1‐72.
Katoh K, Rozewicki J, Yamada KD. MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Brief Bioinform. 2019;20:1160‐1166.
Castresana J. Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol. 2000;17:540‐552.
Stielow JB, Lévesque CA, Seifert KA, et al. One fungus, which genes? Development and assessment of universal primers for potential secondary fungal DNA barcodes. Persoonia. 2015;35:242‐263.
Edler D, Klein J, Antonelli A, Silvestro D. raxmlGUI 2.0: a graphical interface and toolkit for phylogenetic analyses using RAxML. MEE. 2021;12:373‐377.
Brasch J, Gräser Y. Trichophyton eboreum sp. nov. isolated from human skin. J Clin Microbiol. 2005;43:5230‐5337.
Brasch J, Beck‐Jendroschek V, Voss K, et al. Arthroderma crocatum auf menschlicher Haut. Hautarzt. 2021;72:267‐270.
Brasch J, Gräser Y, Drerup K, Voss K, Schwarz T. First genetically confirmed isolation of Pseudogymnoascus appendiculatus in Europe ‐ surprisingly from psoriatic human skin. J Dtsch Dermatol Ges. 2023;21:60‐62.
Brasch J, Zaldua M. Enzyme patterns of dermatophytes. Mycoses. 1994;37:11‐16.
Kunert J. Physiology of keratinophilic fungi. In: Kushwaha RKS, Guarro J, eds. Biology of Dermatophytes and Other Keratinophilic Fungi. Revista Iberoamericana de Micologia; 2000:77‐85.
Dukik K, de Hoog GS, Stielow JB, et al. Molecular and phenotypic characterization of Nannizzia (Arthrodermataceae). Mycopathologia. 2020;185:9‐35.
Sharma R, Gräser Y, Singh SK. Auxarthronopsis, a new genus of Onygenales isolated from the vicinity of Bandhavgarh national park. India IMA Fungus. 2013;4:89‐102.
Orr GF, Kuehn HH, Varsavsky E. The genus Amauroascus. Mycopathol Mycol Appl. 1965;25:100‐108.
de Hoog S, Walsh TJ, Ahmed SA, Alastruey‐Izquierdo A, Alexander BD, et al. A conceptual framework for nomenclatural stability and validity of medically important fungi: a proposed global consensus guideline for fungal name changes supported by ABP, ASM, CLSI, ECMM, ESCMID‐EFISG, EUCAST‐AFST, FDLC, IDSA, ISHAM, MMSA, and MSGERC. J Clin Microbiol. 2023;61(11):e0087323. doi:10.1128/jcm.00873-23.
Kidd SE, Abdolrasouli A, Hagen F. Fungal nomenclature: managing change is the name of the game. Open Forum Infect Dis. 2023;10(1):ofac559. doi:10.1093/ofid/ofac559.
فهرسة مساهمة: Keywords: Neogymnomycetaceae; genotype; phenotype; phylogeny; physiology; taxonomy
المشرفين على المادة: 0 (DNA, Fungal)
0 (DNA, Ribosomal Spacer)
68238-35-7 (Keratins)
0 (DNA, Ribosomal)
تواريخ الأحداث: Date Created: 20240802 Date Completed: 20240802 Latest Revision: 20240802
رمز التحديث: 20240802
DOI: 10.1111/myc.13774
PMID: 39092516
قاعدة البيانات: MEDLINE
الوصف
تدمد:1439-0507
DOI:10.1111/myc.13774