دورية أكاديمية
Detection of Mycobacterium tuberculosis complex in saliva by quantitative PCR: A potential alternative specimen for pulmonary tuberculosis diagnosis.
| العنوان: | Detection of Mycobacterium tuberculosis complex in saliva by quantitative PCR: A potential alternative specimen for pulmonary tuberculosis diagnosis. |
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| المؤلفون: | Ayalew S; Armauer Hansen Research Institute, Addis Ababa, Ethiopia; Department of Biology, College of Natural and Computational Sciences, Arba Minch University, Arba Minch, Ethiopia. Electronic address: absosina2011@gmail.com., Wegayehu T; Department of Biology, College of Natural and Computational Sciences, Arba Minch University, Arba Minch, Ethiopia. Electronic address: tekluweg2007@yahoo.com., Wondale B; Department of Biology, College of Natural and Computational Sciences, Arba Minch University, Arba Minch, Ethiopia. Electronic address: biniam.wondale@amu.edu.et., Kebede D; Armauer Hansen Research Institute, Addis Ababa, Ethiopia. Electronic address: dawitkmaau@gmail.com., Osman M; Armauer Hansen Research Institute, Addis Ababa, Ethiopia. Electronic address: mahi2b4@yahoo.com., Niway S; Armauer Hansen Research Institute, Addis Ababa, Ethiopia. Electronic address: sebsibneway2152@gmail.com., Tarekegn A; Armauer Hansen Research Institute, Addis Ababa, Ethiopia. Electronic address: azititar@gmail.com., Tessema B; Armauer Hansen Research Institute, Addis Ababa, Ethiopia. Electronic address: bamlak.tessema@yahoo.com., Berg S; Department of Bacteriology, Animal and Plant Health Agency, Weybridge, United Kingdom; Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany. Electronic address: stefan.berg@bnitm.de., Ashford RT; Department of Bacteriology, Animal and Plant Health Agency, Weybridge, United Kingdom. Electronic address: roland.ashford@apha.gov.uk., Mihret A; Armauer Hansen Research Institute, Addis Ababa, Ethiopia. Electronic address: adane.mihret@ahri.gov.et. |
| المصدر: | Tuberculosis (Edinburgh, Scotland) [Tuberculosis (Edinb)] 2024 Jul 31; Vol. 148, pp. 102554. Date of Electronic Publication: 2024 Jul 31. |
| Publication Model: | Ahead of Print |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Churchill Livingstone Country of Publication: Scotland NLM ID: 100971555 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-281X (Electronic) Linking ISSN: 14729792 NLM ISO Abbreviation: Tuberculosis (Edinb) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Edinburgh, New York : Churchill Livingstone, c2001- |
| مستخلص: | Background: Current tuberculosis (TB) diagnostic tests primarily rely on sputum samples, yet many TB patients cannot produce sputum. This study explored whether saliva could be used instead of sputum to diagnose pulmonary TB (PTB). Method: The study included 32 patients with confirmed PTB and 30 patients with other respiratory diseases (ORD). Saliva from all study participants was subjected to quantitative (qPCR) assays targeting the IS1081 gene for detection of M. tuberculosis complex DNA. Results: The sensitivity of saliva IS1081 qPCR was 65.6 % (95 % CI 48.4-80.2 %) with positive results for 21/32 PTB cases, while the specificity was 96.7 % (95 % CI 85.9-99.6 %) with negative results for 29/30 participants with ORD. Sensitivity improved to 72.4 % (95 % CI 54.6-86.0 %) when sputum-Xpert was used as the reference standard, while remaining similar at 65.5 % (95 % CI 47.4-80.7 %) when culture was used as the reference standard. In receiver operating characteristic (ROC) curve analysis, the area under the curve (AUC) for saliva IS1081 qPCR was 82.5 % (95 % CI 71.7-93.3 %). Conclusion: Saliva testing offers a promising alternative to sputum for TB diagnosis among confirmed PTB cases. Larger multicenter studies, encompassing diverse clinical TB characteristics, are needed to provide improved estimates of diagnostic sensitivity and specificity. Competing Interests: Declaration of competing interest None (Copyright © 2024. Published by Elsevier Ltd.) |
| فهرسة مساهمة: | Keywords: IS1081; Mycobacterium tuberculosis complex; Pulmonary tuberculosis; Quantitative PCR; saliva |
| تواريخ الأحداث: | Date Created: 20240802 Latest Revision: 20240802 |
| رمز التحديث: | 20240803 |
| DOI: | 10.1016/j.tube.2024.102554 |
| PMID: | 39094295 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1873-281X |
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| DOI: | 10.1016/j.tube.2024.102554 |