دورية أكاديمية
Construction of a Calibration Curve for Lycopene on a Liquid-Handling Platform─Wider Lessons for the Development of Automated Dilution Protocols.
العنوان: | Construction of a Calibration Curve for Lycopene on a Liquid-Handling Platform─Wider Lessons for the Development of Automated Dilution Protocols. |
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المؤلفون: | Bultelle M; Department of Bioengineering, Imperial College London, London SW7 2BX, United Kingdom., Casas A; Department of Bioengineering, Imperial College London, London SW7 2BX, United Kingdom., Kitney R; Department of Bioengineering, Imperial College London, London SW7 2BX, United Kingdom. |
المصدر: | ACS synthetic biology [ACS Synth Biol] 2024 Aug 16; Vol. 13 (8), pp. 2357-2375. Date of Electronic Publication: 2024 Aug 03. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101575075 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2161-5063 (Electronic) Linking ISSN: 21615063 NLM ISO Abbreviation: ACS Synth Biol Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Washington, D.C. : American Chemical Society, c2012- |
مواضيع طبية MeSH: | Lycopene* , Dimethyl Sulfoxide*/chemistry, Calibration ; Automation ; Carotenoids/analysis ; Synthetic Biology/methods |
مستخلص: | Liquid-handling is a fundamental operation in synthetic biology─all protocols involve one or more liquid-handling operations. It is, therefore, crucial that this step be carefully automated in order to unlock the benefits of automation (e.g., higher throughput, higher replicability). In the paper, we present a study, conducted at the London Biofoundry at SynbiCITE, that approaches liquid-handling and its reliable automation from the standpoint of the construction of the calibration curve for lycopene in dimethyl sulfoxide (DMSO). The study has important practical industrial applications (e.g., lycopene is a carotenoid of industrial interest, DMSO is a popular extractant). The study was also an effective testbed for the automation of liquid-handling. It necessitated the development of flexible liquid-handling methods, which can be generalizable to other automated applications. In addition, because lycopene/DMSO is a difficult mix, it was capable of revealing issues with automated liquid-handling protocols and stress-testing them. An important component of the study is the constraint that, due to the omnipresence of liquid-handling steps, errors should be controlled to a high standard. It is important to avoid such errors propagating to other parts of the protocol. To achieve this, a practical framework based on regression was developed and utilized throughout the study to identify, assess, and monitor transfer errors. The paper concludes with recommendations regarding automation of liquid-handling, which are applicable to a large set of applications (not just to complex liquids such as lycopene in DMSO or calibration curves). |
References: | Cornell Vet. 1986 Jan;76(1):61-90. (PMID: 3510103) Front Microbiol. 2017 Nov 20;8:2233. (PMID: 29276501) PLoS Med. 2005 Aug;2(8):e124. (PMID: 16060722) Hum Mov Sci. 2021 Aug;78:102818. (PMID: 34049152) Nature. 2016 May 25;533(7604):452-4. (PMID: 27225100) ACS Synth Biol. 2017 Feb 17;6(2):375-380. (PMID: 27797498) J Microbiol Methods. 2014 Dec;107:214-21. (PMID: 25205541) Clin Lab Med. 2007 Mar;27(1):123-38. (PMID: 17416306) Medicine (Baltimore). 2015 Aug;94(33):e1260. (PMID: 26287411) Synth Biol (Oxf). 2023 Mar 28;8(1):ysad005. (PMID: 37073283) Dis Model Mech. 2014 Feb;7(2):171-3. (PMID: 24713271) AMB Express. 2015 Dec;5(1):65. (PMID: 26395597) J Food Sci. 2016 Nov;81(11):C2630-C2638. (PMID: 27681032) Free Radic Biol Med. 1997;23(7):1086-9. (PMID: 9358253) Food Chem. 2023 Mar 30;405(Pt B):134885. (PMID: 36442244) Can J Comp Med Vet Sci. 1966 Jan;30(1):3-8. (PMID: 4223708) Curr Opin Microbiol. 2016 Oct;33:123-130. (PMID: 27494248) Front Bioeng Biotechnol. 2020 Nov 13;8:571777. (PMID: 33282848) Trends Biotechnol. 2022 Jul;40(7):831-842. (PMID: 35012773) Microb Cell Fact. 2019 Mar 18;18(1):55. (PMID: 30885243) PLoS One. 2018 Jun 21;13(6):e0199432. (PMID: 29928012) Atherosclerosis. 2017 Feb;257:100-108. (PMID: 28129549) Nucleic Acids Res. 2021 Dec 2;49(21):e123. (PMID: 34554258) Commun Biol. 2018 Jun 8;1:66. (PMID: 30271948) J Lab Autom. 2012 Jun;17(3):169-85. (PMID: 22357568) Nat Commun. 2019 Nov 13;10(1):5150. (PMID: 31723141) Commun Biol. 2020 Sep 17;3(1):512. (PMID: 32943734) Nature. 2019 May;569(7757):587-588. (PMID: 31110319) Biochem Med (Zagreb). 2017 Oct 15;27(3):030201. (PMID: 29180912) ACS Synth Biol. 2019 Sep 20;8(9):2080-2091. (PMID: 31386355) J Am Chem Soc. 2006 Nov 1;128(43):13982-3. (PMID: 17061853) Molecules. 2010 Apr 08;15(4):2478-90. (PMID: 20428057) Synth Biol (Oxf). 2022 Oct 06;7(1):ysac018. (PMID: 36285185) J Biol Eng. 2019 Jan 18;13:8. (PMID: 30675181) Integr Biol (Camb). 2011 Feb;3(2):97-108. (PMID: 21258712) Science. 2014 Jan 17;343(6168):229. (PMID: 24436391) Nat Commun. 2019 May 9;10(1):2040. (PMID: 31068573) Front Bioeng Biotechnol. 2014 Nov 26;2:60. (PMID: 25505788) Biosci Biotechnol Biochem. 1994 Jun;58(6):1112-4. (PMID: 7765036) Front Bioeng Biotechnol. 2019 Feb 11;7:18. (PMID: 30805337) Foods. 2019 Apr 09;8(4):. (PMID: 30970532) |
فهرسة مساهمة: | Keywords: calibration curve; dilution; liquid handling-automation; lycopene; metrology |
المشرفين على المادة: | SB0N2N0WV6 (Lycopene) YOW8V9698H (Dimethyl Sulfoxide) 36-88-4 (Carotenoids) |
تواريخ الأحداث: | Date Created: 20240803 Date Completed: 20240816 Latest Revision: 20240822 |
رمز التحديث: | 20240822 |
مُعرف محوري في PubMed: | PMC11334188 |
DOI: | 10.1021/acssynbio.4c00031 |
PMID: | 39096303 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2161-5063 |
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DOI: | 10.1021/acssynbio.4c00031 |