دورية أكاديمية
Construction of a Calibration Curve for Lycopene on a Liquid-Handling Platform─Wider Lessons for the Development of Automated Dilution Protocols.
| العنوان: | Construction of a Calibration Curve for Lycopene on a Liquid-Handling Platform─Wider Lessons for the Development of Automated Dilution Protocols. |
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| المؤلفون: | Bultelle M; Department of Bioengineering, Imperial College London, London SW7 2BX, United Kingdom., Casas A; Department of Bioengineering, Imperial College London, London SW7 2BX, United Kingdom., Kitney R; Department of Bioengineering, Imperial College London, London SW7 2BX, United Kingdom. |
| المصدر: | ACS synthetic biology [ACS Synth Biol] 2024 Aug 16; Vol. 13 (8), pp. 2357-2375. Date of Electronic Publication: 2024 Aug 03. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101575075 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2161-5063 (Electronic) Linking ISSN: 21615063 NLM ISO Abbreviation: ACS Synth Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, D.C. : American Chemical Society, c2012- |
| مواضيع طبية MeSH: | Lycopene* , Dimethyl Sulfoxide*/chemistry, Calibration ; Automation ; Carotenoids/analysis ; Synthetic Biology/methods |
| مستخلص: | Liquid-handling is a fundamental operation in synthetic biology─all protocols involve one or more liquid-handling operations. It is, therefore, crucial that this step be carefully automated in order to unlock the benefits of automation (e.g., higher throughput, higher replicability). In the paper, we present a study, conducted at the London Biofoundry at SynbiCITE, that approaches liquid-handling and its reliable automation from the standpoint of the construction of the calibration curve for lycopene in dimethyl sulfoxide (DMSO). The study has important practical industrial applications (e.g., lycopene is a carotenoid of industrial interest, DMSO is a popular extractant). The study was also an effective testbed for the automation of liquid-handling. It necessitated the development of flexible liquid-handling methods, which can be generalizable to other automated applications. In addition, because lycopene/DMSO is a difficult mix, it was capable of revealing issues with automated liquid-handling protocols and stress-testing them. An important component of the study is the constraint that, due to the omnipresence of liquid-handling steps, errors should be controlled to a high standard. It is important to avoid such errors propagating to other parts of the protocol. To achieve this, a practical framework based on regression was developed and utilized throughout the study to identify, assess, and monitor transfer errors. The paper concludes with recommendations regarding automation of liquid-handling, which are applicable to a large set of applications (not just to complex liquids such as lycopene in DMSO or calibration curves). |
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| فهرسة مساهمة: | Keywords: calibration curve; dilution; liquid handling-automation; lycopene; metrology |
| المشرفين على المادة: | SB0N2N0WV6 (Lycopene) YOW8V9698H (Dimethyl Sulfoxide) 36-88-4 (Carotenoids) |
| تواريخ الأحداث: | Date Created: 20240803 Date Completed: 20240816 Latest Revision: 20240822 |
| رمز التحديث: | 20240822 |
| مُعرف محوري في PubMed: | PMC11334188 |
| DOI: | 10.1021/acssynbio.4c00031 |
| PMID: | 39096303 |
| قاعدة البيانات: | MEDLINE |
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