دورية أكاديمية
Harmonization of experimental procedures to assess mitochondrial respiration in human permeabilized skeletal muscle fibers.
| العنوان: | Harmonization of experimental procedures to assess mitochondrial respiration in human permeabilized skeletal muscle fibers. |
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| المؤلفون: | Doerrier C; Oroboros Instruments, Schöpfstrasse 18, 6020 Innsbruck, Austria. Electronic address: carolina.doerrier@gmail.com., Gama-Perez P; Dept Physiological Sciences, Univ Barcelona and Bellvitge Biomedical Research Inst, Spain. Electronic address: p.gamaperez@gmail.com., Pesta D; Inst Clinical Diabetology, German Diabetes Center, Leibniz Center Diabetes Research Heinrich-Heine Univ Düsseldorf, Germany; German Center Diabetes Research, Munich, Neuherberg, Germany; Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; Center for Endocrinology, Diabetes and Preventive Medicine (CEDP), University Hospital Cologne, Cologne, Germany; Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), Cologne, Germany. Electronic address: Dominik.Pesta@ddz.uni-duesseldorf.de., Distefano G; Translational Research Institute AdventHealth, Orlando, FL, USA. Electronic address: Giovanna.Distefano@adventhealth.com., Soendergaard SD; Xlab, Dept Biomedical Sciences, Center Healthy Aging, Fac Health Sciences, Denmark. Electronic address: wdz320@alumni.ku.dk., Chroeis KM; Xlab, Dept Biomedical Sciences, Center Healthy Aging, Fac Health Sciences, Denmark. Electronic address: rwn105@alumni.ku.dk., Gonzalez-Franquesa A; The Novo Nordisk Center Basic Metabolic Research, Section Integrative Physiology, Univ Copenhagen, Denmark. Electronic address: agonzalezfranquesa@gmail.com., Goodpaster BH; Translational Research Institute AdventHealth, Orlando, FL, USA. Electronic address: bret.goodpaster@adventhealth.com., Prats C; Dept Biomedical Sciences, Center Healthy Aging, Fac Health Sciences, Denmark; The Core Facility for Integrated Microscopy, Faculty of Health and Medical Sciences, University of Copenhagen. Electronic address: cprats@sund.ku.dk., Sales-Pardo M; Dept of Chemical Engineering, Universitat Rovira i Virgili, Tarragona, Spain. Electronic address: marta.sales@urv.cat., Guimera R; Dept of Chemical Engineering, Universitat Rovira i Virgili, Tarragona, Spain; Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain. Electronic address: roger.guimera@urv.cat., Coen PM; Translational Research Institute AdventHealth, Orlando, FL, USA. Electronic address: Paul.Coen@adventhealth.com., Gnaiger E; Oroboros Instruments, Schöpfstrasse 18, 6020 Innsbruck, Austria. Electronic address: erich.gnaiger@oroboros.at., Larsen S; Xlab, Dept Biomedical Sciences, Center Healthy Aging, Fac Health Sciences, Denmark; Clinical Research Centre, Medical University of Bialystok, Bialystok, Poland. Electronic address: stelar@sund.ku.dk., Garcia-Roves PM; Dept Physiological Sciences, Univ Barcelona and Bellvitge Biomedical Research Inst, Spain. Electronic address: pgarciaroves@ub.edu. |
| المصدر: | Free radical biology & medicine [Free Radic Biol Med] 2024 Aug 01. Date of Electronic Publication: 2024 Aug 01. |
| Publication Model: | Ahead of Print |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: United States NLM ID: 8709159 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4596 (Electronic) Linking ISSN: 08915849 NLM ISO Abbreviation: Free Radic Biol Med Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Tarrytown, NY : Elsevier Science Original Publication: New York : Pergamon, c1987- |
| مستخلص: | Aim: High-resolution respirometry in human permeabilized muscle fibers is extensively used for analysis of mitochondrial adaptions to nutrition and exercise interventions, and is linked to athletic performance. However, the lack of standardization of experimental conditions limits quantitative inter- and intra-laboratory comparisons. Methods: In our study, an international team of investigators measured mitochondrial respiration of permeabilized muscle fibers obtained from three biopsies (vastus lateralis) from the same healthy volunteer to avoid inter-individual variability. High-resolution respirometry assays were performed together at the same laboratory to assess whether the heterogenity in published results are due to the effects of respiration media (MiR05 versus Z) with or without the myosin inhibitor blebbistatin at low- and high-oxygen regimes. Results: Our findings reveal significant differences between respiration media for OXPHOS and ET capacities supported by NADH&succinate-linked substrates at different oxygen concentrations. Respiratory capacities were approximately 1.5-fold higher in MiR05 at high-oxygen regimes compared to medium Z near air saturation. The presence or absence of blebbistatin in human permeabilized muscle fiber preparations was without effect on oxygen flux. Conclusion: Our study constitutes a basis to harmonize and establish optimum experimental conditions for respirometric studies of permeabilized human skeletal muscle fibers to improve reproducibility. Competing Interests: Declaration of Competing Interest The authors declare no competing interests. (Copyright © 2024. Published by Elsevier Inc.) |
| فهرسة مساهمة: | Keywords: O(2) regime; blebbistatin; high-resolution respirometry; mitochondrial respiration; permeabilized skeletal muscle fibers; respiration medium MiR05; respiration medium Z |
| تواريخ الأحداث: | Date Created: 20240803 Latest Revision: 20240803 |
| رمز التحديث: | 20240804 |
| DOI: | 10.1016/j.freeradbiomed.2024.07.039 |
| PMID: | 39097206 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-4596 |
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| DOI: | 10.1016/j.freeradbiomed.2024.07.039 |