دورية أكاديمية
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Characterization of a vacuolar importer of secologanin in Catharanthus roseus.

التفاصيل البيبلوغرافية
العنوان: Characterization of a vacuolar importer of secologanin in Catharanthus roseus.
المؤلفون: Li F; Plant Science, McGill University, Sainte-Anne-de-Bellevue, QC, H9X 3V9, Canada., Shahsavarani M; Chemistry, University of New Brunswick, Fredericton, NB, E3B 5A3, Canada., Handy-Hart CJ; Parasitology, McGill University, Sainte-Anne-de-Bellevue, QC, H9X 3V9, Canada., Côté A; Plant Science, McGill University, Sainte-Anne-de-Bellevue, QC, H9X 3V9, Canada., Brasseur-Trottier X; Plant Science, McGill University, Sainte-Anne-de-Bellevue, QC, H9X 3V9, Canada., Montgomery V; Parasitology, McGill University, Sainte-Anne-de-Bellevue, QC, H9X 3V9, Canada., Beech RN; Parasitology, McGill University, Sainte-Anne-de-Bellevue, QC, H9X 3V9, Canada., Liu L; Food Science and Agricultural Chemistry, McGill University, Sainte-Anne-de-Bellevue, QC, H9X 3V9, Canada., Bayen S; Food Science and Agricultural Chemistry, McGill University, Sainte-Anne-de-Bellevue, QC, H9X 3V9, Canada., Qu Y; Chemistry, University of New Brunswick, Fredericton, NB, E3B 5A3, Canada., De Luca V; Biological Sciences, Brock University, St. Catharines, ON, L2S 3A1, Canada., Dastmalchi M; Plant Science, McGill University, Sainte-Anne-de-Bellevue, QC, H9X 3V9, Canada. mehran.dastmalchi@mcgill.ca.
المصدر: Communications biology [Commun Biol] 2024 Aug 03; Vol. 7 (1), pp. 939. Date of Electronic Publication: 2024 Aug 03.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group UK Country of Publication: England NLM ID: 101719179 Publication Model: Electronic Cited Medium: Internet ISSN: 2399-3642 (Electronic) Linking ISSN: 23993642 NLM ISO Abbreviation: Commun Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London, United Kingdom : Nature Publishing Group UK, [2018]-
مواضيع طبية MeSH: Catharanthus*/metabolism , Catharanthus*/genetics , Plant Proteins*/metabolism , Plant Proteins*/genetics , Vacuoles*/metabolism , Secologanin Tryptamine Alkaloids*/metabolism, Animals ; Phylogeny ; Xenopus laevis/metabolism ; Biological Transport ; Oocytes/metabolism ; Iridoid Glucosides
مستخلص: Monoterpenoid indole alkaloid (MIA) biosynthesis in Catharanthus roseus is a paragon of the spatiotemporal complexity achievable by plant specialized metabolism. Spanning a range of tissues, four cell types, and five cellular organelles, MIA metabolism is intricately regulated and organized. This high degree of metabolic differentiation requires inter-cellular and organellar transport, which remains understudied. Here, we have characterized a vacuolar importer of secologanin belonging to the multidrug and toxic compound extrusion (MATE) family, named CrMATE1. Phylogenetic analyses of MATEs suggested a role in alkaloid transport for CrMATE1, and in planta silencing in two varieties of C. roseus resulted in a shift in the secoiridoid and MIA profiles. Subcellular localization of CrMATE1 confirmed tonoplast localization. Biochemical characterization was conducted using the Xenopus laevis oocyte expression system to determine substrate range, directionality, and rate. We can confirm that CrMATE1 is a vacuolar importer of secologanin, translocating 1 mM of substrate within 25 min. The transporter displayed strict directionality and specificity for secologanin and did not accept other secoiridoid substrates. The unique substrate-specific activity of CrMATE1 showcases the utility of transporters as gatekeepers of pathway flux, mediating the balance between a defense arsenal and cellular homeostasis.
(© 2024. The Author(s).)
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معلومات مُعتمدة: RGPIN-2021-02817 Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada (Conseil de Recherches en Sciences Naturelles et en Génie du Canada)
المشرفين على المادة: 0 (Plant Proteins)
0 (Secologanin Tryptamine Alkaloids)
19351-63-4 (secologanin)
0 (Iridoid Glucosides)
تواريخ الأحداث: Date Created: 20240803 Date Completed: 20240803 Latest Revision: 20240816
رمز التحديث: 20240816
مُعرف محوري في PubMed: PMC11298008
DOI: 10.1038/s42003-024-06624-5
PMID: 39097635
قاعدة البيانات: MEDLINE
الوصف
تدمد:2399-3642
DOI:10.1038/s42003-024-06624-5