دورية أكاديمية
Differential composition and yield of leukocytes isolated from various blood component leukoreduction filters.
| العنوان: | Differential composition and yield of leukocytes isolated from various blood component leukoreduction filters. |
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| المؤلفون: | Six KR; Transfusion Research Center, Belgian Red Cross Flanders, Ghent, Belgium. Electronic address: katrijn.six@rodekruis.be., Vertongen S; Transfusion Research Center, Belgian Red Cross Flanders, Ghent, Belgium., Seghers S; Transfusion Innovation Center, Belgian Red Cross Flanders, Ghent, Belgium., De Bleser D; Transfusion Innovation Center, Belgian Red Cross Flanders, Ghent, Belgium., Compernolle V; Transfusion Research Center, Belgian Red Cross Flanders, Ghent, Belgium; Transfusion Innovation Center, Belgian Red Cross Flanders, Ghent, Belgium; Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium; Blood Services, Belgian Red Cross Flanders, Mechelen, Belgium., Feys HB; Transfusion Research Center, Belgian Red Cross Flanders, Ghent, Belgium; Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium. |
| المصدر: | Journal of immunological methods [J Immunol Methods] 2024 Aug 02; Vol. 533, pp. 113733. Date of Electronic Publication: 2024 Aug 02. |
| Publication Model: | Ahead of Print |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 1305440 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7905 (Electronic) Linking ISSN: 00221759 NLM ISO Abbreviation: J Immunol Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Amsterdam, North-Holand, |
| مستخلص: | In Flanders, an estimated 300,000 leukoreduction filters are discarded as biological waste in the blood establishment each year. These filters are a possible source of fresh donor leukocytes for downstream purposes including research. We investigated leukocyte isolation from two types of filters either used for the preparation of platelet concentrates (PC-LRF) or erythrocyte concentrates (EC-LRF). Outcome parameters were leukocyte yield, differential count, turnaround time and effect of storage conditions. Leukocytes were harvested by reverse flow of a buffer solution. Control was the gold standard density gradient centrifugation of buffy coats. Total leukocyte number isolated from PC-LRF (1049 (± 40) x 10 6 ) was almost double that of control (632 (± 66) x 10 6 ) but the differential count was comparable. Total leukocyte number isolated from EC-LRF (78 (± 9) x 10 6 ) was significantly lower than control, but the sample was specifically enriched in granulocytes (81 ± 4%) compared to control (30 ± 1%). Isolation of leukocytes from either PC- or EC-LRF takes 20 min compared to 240 min for control density gradient centrifugation. Leukocyte viability is optimal when harvested on day 1 post donation (95 ± 0.9%) compared to day 3 (76.4 ± 2.4%). In conclusion, our study demonstrates that leukoreduction filters from specific blood component processing are easy to use and present a valuable source for viable leukocytes of all types. Competing Interests: Declaration of competing interest The authors declare no conflicts of interest in relation to this work. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Blood processing; Leukocyte filter; Leukocytes |
| تواريخ الأحداث: | Date Created: 20240804 Latest Revision: 20240822 |
| رمز التحديث: | 20240823 |
| DOI: | 10.1016/j.jim.2024.113733 |
| PMID: | 39098592 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-7905 |
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| DOI: | 10.1016/j.jim.2024.113733 |