دورية أكاديمية
أعرض في EDS

Genome-wide identification and evolution of the SAP gene family in sunflower ( Helianthus annuus L.) and expression analysis under salt and drought stress.

التفاصيل البيبلوغرافية
العنوان: Genome-wide identification and evolution of the SAP gene family in sunflower ( Helianthus annuus L.) and expression analysis under salt and drought stress.
المؤلفون: Zhang C; Hetao College, Bayannur, China., Zhang X; Bayannur Institute of Agriculture and Animal Science, Bayannur, China., Wu Y; Bayannur Institute of Agriculture and Animal Science, Bayannur, China., Li X; Hetao College, Bayannur, China., Du C; Bayannur Institute of Agriculture and Animal Science, Bayannur, China., Di N; Hetao College, Bayannur, China., Chen Y; Hetao College, Bayannur, China.
المصدر: PeerJ [PeerJ] 2024 Jul 30; Vol. 12, pp. e17808. Date of Electronic Publication: 2024 Jul 30 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: PeerJ Inc Country of Publication: United States NLM ID: 101603425 Publication Model: eCollection Cited Medium: Internet ISSN: 2167-8359 (Electronic) Linking ISSN: 21678359 NLM ISO Abbreviation: PeerJ Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Corte Madera, CA : PeerJ Inc.
مواضيع طبية MeSH: Helianthus*/genetics , Helianthus*/metabolism , Plant Proteins*/genetics , Plant Proteins*/metabolism , Droughts* , Phylogeny* , Gene Expression Regulation, Plant* , Multigene Family* , Evolution, Molecular*, Stress, Physiological/genetics ; Genome, Plant ; Salt Stress/genetics
مستخلص: Stress-associated proteins (SAPs) are known to play an important role in plant responses to abiotic stresses. This study systematically identified members of the sunflower SAP gene family using sunflower genome data. The genes of the sunflower SAP gene family were analyzed using bioinformatic methods, and gene expression was assessed through fluorescence quantification (qRT-PCR) under salt and drought stress. A comprehensive analysis was also performed on the number, structure, collinearity, and phylogeny of seven Compositae species and eight other plant SAP gene families. The sunflower genome was found to have 27 SAP genes, distributed across 14 chromosomes. The evolutionary analysis revealed that the SAP family genes could be divided into three subgroups. Notably, the annuus variety exhibited amplification of the SAP gene for Group 3. Among the Compositae species, C. morifolium demonstrated the highest number of collinearity gene pairs and the closest distance on the phylogenetic tree, suggesting relative conservation in the evolutionary process. An analysis of gene structure revealed that Group 1 exhibited the most complex gene structure, while the majority of HaSAP genes in Group 2 and Group 3 lacked introns. The promoter analysis revealed the presence of cis-acting elements related to ABA, indicating their involvement in stress responses. The expression analysis indicated the potential involvement of 10 genes ( HaSAP1, HaSAP3, HaSAP8, HaSAP10, HaSAP15, HaSAP16, HaSAP21, HaSAP22, HaSAP23 , and HaSAP26 ) in sunflower salt tolerance. The expression of these 10 genes were then examined under salt and drought stress using qRT-PCR, and the tissue-specific expression patterns of these 10 genes were also analyzed. HaSAP1 , HaSAP21 , and HaSAP23 exhibited consistent expression patterns under both salt and drought stress, indicating these genes play a role in both salt tolerance and drought resistance in sunflower. The findings of this study highlight the significant contribution of the SAP gene family to salt tolerance and drought resistance in sunflower.
Competing Interests: The authors declare that they have no competing interests.
(© 2024 Zhang et al.)
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فهرسة مساهمة: Keywords: Evolutionary analysis; SAP; Sunflower; qRT-PCR
المشرفين على المادة: 0 (Plant Proteins)
تواريخ الأحداث: Date Created: 20240805 Date Completed: 20240805 Latest Revision: 20240806
رمز التحديث: 20240806
مُعرف محوري في PubMed: PMC11296301
DOI: 10.7717/peerj.17808
PMID: 39099650
قاعدة البيانات: MEDLINE
الوصف
تدمد:2167-8359
DOI:10.7717/peerj.17808