دورية أكاديمية
أعرض في EDS

Characterization of the dominant strain (G-VII) of Newcastle disease viruses isolated from commercial chickens in Bangladesh during recent outbreaks.

التفاصيل البيبلوغرافية
العنوان: Characterization of the dominant strain (G-VII) of Newcastle disease viruses isolated from commercial chickens in Bangladesh during recent outbreaks.
المؤلفون: Haque MA; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh., Sadekuzzaman M; Central Disease Investigation Laboratory, Department of Livestock Services, Dhaka, Bangladesh., Haque ME; Department of Microbiology, Sheikh Hasina University of Science and Technology, Bhairab, Bangladesh., Parvin MK; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh., Kamal MM; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh., Hayat S; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh., Islam MA; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh., Khatun MM; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh., Siddique MP; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh., Nahar SS; Livestock Research Institute, Mohakhali, Dhaka, Bangladesh., Khasruzzaman AKM; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh., Hossain MT; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh., Islam MA; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
المصدر: Journal of advanced veterinary and animal research [J Adv Vet Anim Res] 2024 Jun 09; Vol. 11 (2), pp. 408-417. Date of Electronic Publication: 2024 Jun 09 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Network for the Veterinarians of Bangladesh Country of Publication: Bangladesh NLM ID: 101647585 Publication Model: eCollection Cited Medium: Internet ISSN: 2311-7710 (Electronic) Linking ISSN: 23117710 NLM ISO Abbreviation: J Adv Vet Anim Res Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Mymensingh : Network for the Veterinarians of Bangladesh, [2014]-
مستخلص: Objective: Newcastle disease virus genotype VII (NDV-GVII), an extremely infectious pathogen, has been causing severe economic consequences for the chicken industry. The current study aimed to isolate and characterize NDV-GVII from commercial chickens in Bangladesh during a recent outbreak.
Materials and Methods: From clinically suspected chickens from 70 commercial poultry farms, a total of 420 samples (trachea, lungs, and brain tissue) were collected. The samples were cultivated in 9-10 day-old seronegative embryonated chicken eggs (ECEs) after evaluating them using the rapid Newcastle disease virus (NDV) antigen detection kit. The hemagglutination (HA) inhibition test, agar gel immune diffusion (AGID) test, molecular detection by reverse transcription-polymerase chain reaction (RT-PCR), and phylogenetic studies using gene sequences of fusion (F) protein. The HA pattern of isolated NDV was determined using different avian and mammalian red blood cells (RBCs). The pathogenicity of the isolated virus was evaluated using mean death time (MDT), intravenous pathogenicity index (IVPI), and intracerebral pathogenicity index (ICPI).
Results: The study found 87 NDV samples positive using the rapid NDV Ag detection kit and then 60 positives for virus isolation in ECEs. All 60 isolates were positive for NDV by HI, AGID, and RT-PCR. Phylogenetic tree analysis indicated that recent NDV isolates belong to genotype VII and exhibit a similarity of 99.7%-98.5% with isolates from Bangladesh, Iran, and India. The new isolates, identified as velogenic strains of NDV, possess an F protein cleavage site with 112 -R-T-K-R-F- 117 amino acid motifs. The isolated NDV showed diversified HA activity while using RBCs from birds and mammals. The results of ICPI, IVPI, and MDT indicated that the recent NDV isolates were very virulent.
Conclusion: This study concluded that NDV-GVII is prevalent in commercial poultry farms in Bangladesh.
Competing Interests: The authors announce that they do not have any conflicts of interest, such as financial, personal, or other connections with individuals or entities, that could potentially affect the content of this manuscript.
(© The authors.)
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فهرسة مساهمة: Keywords: ICPI; IVPI; MDT genotype VII; NDV; RT-PCR
تواريخ الأحداث: Date Created: 20240805 Latest Revision: 20240806
رمز التحديث: 20240806
مُعرف محوري في PubMed: PMC11296175
DOI: 10.5455/javar.2024.k790
PMID: 39101070
قاعدة البيانات: MEDLINE
الوصف
تدمد:2311-7710
DOI:10.5455/javar.2024.k790