دورية أكاديمية
أعرض في EDS

Characterization of infectious laryngotracheitis virus isolated from commercial layer chickens in Bangladesh during the year 2021-2022.

التفاصيل البيبلوغرافية
العنوان: Characterization of infectious laryngotracheitis virus isolated from commercial layer chickens in Bangladesh during the year 2021-2022.
المؤلفون: Kamal MM; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh., Sadekuzzaman M; Central Disease Investigation Laboratory, Department of Livestock Services, Dhaka, Bangladesh., Parvin K; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh., Haque ME; Department of Microbiology, Sheikh Hasina University of Science and Technology, Bhairab, Bangladesh., Hayat S; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh., Islam MA; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh., Khatun MM; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh., Siddique MP; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh., Nahar SS; Livestock Research Institute, Mohakhali, Dhaka, Bangladesh., Khasruzzaman AKM; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh., Hossain MT; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh., Islam MA; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
المصدر: Journal of advanced veterinary and animal research [J Adv Vet Anim Res] 2024 Jun 09; Vol. 11 (2), pp. 398-407. Date of Electronic Publication: 2024 Jun 09 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Network for the Veterinarians of Bangladesh Country of Publication: Bangladesh NLM ID: 101647585 Publication Model: eCollection Cited Medium: Internet ISSN: 2311-7710 (Electronic) Linking ISSN: 23117710 NLM ISO Abbreviation: J Adv Vet Anim Res Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Mymensingh : Network for the Veterinarians of Bangladesh, [2014]-
مستخلص: Objective: Infectious laryngotracheitis virus (ILTV) is responsible for causing infectious laryngotracheitis (ILT), which is a rapidly spreading and extremely transmissible disease in chickens. The current research aims to isolate and characterize ILTV from layer chickens in Bangladesh.
Materials and Methods: A total of 345 samples (trachea, larynx, and lungs) were collected from ILT-suspected dead and sick layer chickens of 32 ILT-suspected farms in three different outbreak districts (Gazipur, Tangail, and Mymensingh) of Bangladesh during the outbreak year 2021-2022. Rapid detection kits examined the samples for avian influenza virus (AIV) and Newcastle disease virus (NDV). ILTV-specific primers were used to screen 72 NDV- and AIV-negative samples by polymerase chain reaction (PCR). Using chorioallantoic membrane (CAM), the study isolated the ILT virus from 9 to 10-day-old seronegative embryonated chicken eggs (ECEs) using selected PCR-positive samples. The virus was confirmed using nucleotide sequencing, agar gel immunodiffusion test (AGIDT), viral neutralization test (VNT), and pathogenicity evaluations using mortality index for chicken embryos (MICEs) and intra-tracheal pathogenicity index (ITPI).
Results: The results indicated that among the PCR-positive 10 samples, only two (Alim_ILT_1001 and Alim_ILT_1,000) were found positive using ECEs. There were two field isolates of ILTVs, as shown by the amplicon size of the ICP4 gene-based PCR. A phylogenetic study of the ICP4 gene revealed that the recent isolates have a close similarity with the ILTV isolates of Turkey, Bangladesh, and Australia. AGIDT revealed strong precipitation lines due to ILTV-specific antibodies reacting with field viruses, while VNT neutralized both isolates with conventional ILTV antibodies. The pathogenicity testing indicated that Alim_ILT_1001 had MICE and ITPI values of 0.77 and 0.63, whereas Alim_ILT_1,000 had 0.71 and 0.57.
Conclusion: Both the ILTV isolates have similarities with the isolates of Turkey, Bangladesh, and Australia, and they are highly virulent for chickens.
Competing Interests: The authors declare that they do not have any competing interests, such as financial, personal, or other connections with individuals or entities, that could potentially affect the content of this manuscript.
(© The authors.)
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فهرسة مساهمة: Keywords: AGIDT; ITPI; Layer chickens; Mice; PCR; VNT
تواريخ الأحداث: Date Created: 20240805 Latest Revision: 20240806
رمز التحديث: 20240806
مُعرف محوري في PubMed: PMC11296171
DOI: 10.5455/javar.2024.k789
PMID: 39101088
قاعدة البيانات: MEDLINE
الوصف
تدمد:2311-7710
DOI:10.5455/javar.2024.k789