دورية أكاديمية
Melatonin modulates TLR4/MyD88/NF-κB signaling pathway to ameliorate cognitive impairment in sleep-deprived rats.
| العنوان: | Melatonin modulates TLR4/MyD88/NF-κB signaling pathway to ameliorate cognitive impairment in sleep-deprived rats. |
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| المؤلفون: | Yin C; School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, China.; Engineering Research Center of TCM Protection Technology and New Product Development for the Elderly Brain Health, Ministry of Education, Hubei University of Chinese Medicine, Wuhan, China.; Hubei Shizhen Laboratory, Hubei University of Chinese Medicine, Wuhan, China., Zhang M; School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, China.; Engineering Research Center of TCM Protection Technology and New Product Development for the Elderly Brain Health, Ministry of Education, Hubei University of Chinese Medicine, Wuhan, China.; Hubei Shizhen Laboratory, Hubei University of Chinese Medicine, Wuhan, China., Cheng L; School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, China., Ding L; School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, China.; Engineering Research Center of TCM Protection Technology and New Product Development for the Elderly Brain Health, Ministry of Education, Hubei University of Chinese Medicine, Wuhan, China.; Hubei Shizhen Laboratory, Hubei University of Chinese Medicine, Wuhan, China., Lv Q; School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, China., Huang Z; School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, China., Zhou J; School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, China., Chen J; School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, China., Wang P; Engineering Research Center of TCM Protection Technology and New Product Development for the Elderly Brain Health, Ministry of Education, Hubei University of Chinese Medicine, Wuhan, China.; Hubei Shizhen Laboratory, Hubei University of Chinese Medicine, Wuhan, China., Zhang S; School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, China., You Q; School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, China.; Engineering Research Center of TCM Protection Technology and New Product Development for the Elderly Brain Health, Ministry of Education, Hubei University of Chinese Medicine, Wuhan, China.; Hubei Shizhen Laboratory, Hubei University of Chinese Medicine, Wuhan, China. |
| المصدر: | Frontiers in pharmacology [Front Pharmacol] 2024 Jul 19; Vol. 15, pp. 1430599. Date of Electronic Publication: 2024 Jul 19 (Print Publication: 2024). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Media] Country of Publication: Switzerland NLM ID: 101548923 Publication Model: eCollection Cited Medium: Print ISSN: 1663-9812 (Print) Linking ISSN: 16639812 NLM ISO Abbreviation: Front Pharmacol Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: [Lausanne : Frontiers Media] |
| مستخلص: | Sleep deprivation (SD) is commonplace in today's fast-paced society. SD is a severe public health problem globally since it may cause cognitive decline and even neurodegenerative disorders like Alzheimer's disease. Melatonin (MT) is a natural chemical secreted by the pineal gland with neuroprotective effects. The purpose of this study was to investigate the protective effect and mechanism of MT on chronic sleep deprivation-induced cognitive impairment. A 3-week modified multi-platform method was used to create the SD rat model. The Morris water maze test (MWM), Tissue staining (including Hematoxylin and Eosin (H & E) staining, Nissl staining, and immunofluorescence), Western blot, Enzyme-linked immunosorbent assay (ELISA), and Quantitative real-time polymerase chain reaction (qPCR) were used to investigate the protective effect and mechanism of MT in ameliorating cognitive impairment in SD rats. The results showed that MT (50 and 100 mg/kg) significantly improved cognitive function in rats, as evidenced by a shortening of escape latency and increased time of crossing the platform and time spent in the quadrant. Additionally, MT therapy alleviated hippocampus neurodegeneration and neuronal loss while lowering levels of pathogenic factors (LPS) and inflammatory indicators (IL-1β, IL-6, TNF-α, iNOS, and COX2). Furthermore, MT treatment reversed the high expression of Aβ42 and Iba1 as well as the low expression of ZO-1 and occludin, and inhibited the SD-induced TLR4/MyD88/NF-κB signaling pathway. In summary, MT ameliorated spatial recognition and learning memory dysfunction in SD rats by reducing neuroinflammation and increasing neuroprotection while inhibiting the TLR4/MyD88/NF-κB signaling pathway. Our study supports the use of MT as an alternate treatment for SD with cognitive impairment. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Yin, Zhang, Cheng, Ding, Lv, Huang, Zhou, Chen, Wang, Zhang and You.) |
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| فهرسة مساهمة: | Keywords: TLR4/MYD88/NF-κB signaling pathway; cognitive impairmen; melatonin; neuroinflammation; sleep deprivation |
| تواريخ الأحداث: | Date Created: 20240805 Latest Revision: 20240806 |
| رمز التحديث: | 20240806 |
| مُعرف محوري في PubMed: | PMC11294086 |
| DOI: | 10.3389/fphar.2024.1430599 |
| PMID: | 39101143 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1663-9812 |
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| DOI: | 10.3389/fphar.2024.1430599 |