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A single-nucleus transcriptomic atlas of medium spiny neurons in the rat nucleus accumbens.

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العنوان: A single-nucleus transcriptomic atlas of medium spiny neurons in the rat nucleus accumbens.
المؤلفون: Reiner BC; Department of Psychiatry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Chehimi SN; Department of Psychiatry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Merkel R; Department of Psychiatry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Department of Biobehavioral Health Sciences, School of Nursing, University of Pennsylvania, Philadelphia, PA, USA., Toikumo S; Department of Psychiatry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Berrettini WH; Department of Psychiatry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Kranzler HR; Department of Psychiatry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Mental Illness Research, Education and Clinical Center, Crescenz VAMC, Philadelphia, PA, USA., Sanchez-Roige S; Department of Psychiatry, University of California San Diego, La Jolla, CA, USA.; Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA.; Institute for Genomic Medicine, University of California San Diego, San Diego, CA, USA., Kember RL; Department of Psychiatry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Mental Illness Research, Education and Clinical Center, Crescenz VAMC, Philadelphia, PA, USA., Schmidt HD; Department of Psychiatry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Department of Biobehavioral Health Sciences, School of Nursing, University of Pennsylvania, Philadelphia, PA, USA., Crist RC; Department of Psychiatry, Perelman School of Medicine, University of Pennsylvania, 125 South 31st Street, Room 2207, Philadelphia, PA, 19104, USA. crist@pennmedicine.upenn.edu.
المصدر: Scientific reports [Sci Rep] 2024 Aug 06; Vol. 14 (1), pp. 18258. Date of Electronic Publication: 2024 Aug 06.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Nucleus Accumbens*/metabolism , Nucleus Accumbens*/cytology , Transcriptome*, Animals ; Humans ; Mice ; Rats ; Cell Nucleus/metabolism ; Cell Nucleus/genetics ; Gene Expression Profiling ; Genome-Wide Association Study ; Medium Spiny Neurons/metabolism ; Single-Cell Analysis
مستخلص: Neural processing of rewarding stimuli involves several distinct regions, including the nucleus accumbens (NAc). The majority of NAc neurons are GABAergic projection neurons known as medium spiny neurons (MSNs). MSNs are broadly defined by dopamine receptor expression, but evidence suggests that a wider array of subtypes exist. To study MSN heterogeneity, we analyzed single-nucleus RNA sequencing data from the largest available rat NAc dataset. Analysis of 48,040 NAc MSN nuclei identified major populations belonging to the striosome and matrix compartments. Integration with mouse and human data indicated consistency across species and disease-relevance scoring using genome-wide association study results revealed potentially differential roles for MSN populations in substance use disorders. Additional high-resolution clustering identified 34 transcriptomically distinct subtypes of MSNs definable by a limited number of marker genes. Together, these data demonstrate the diversity of MSNs in the NAc and provide a basis for more targeted genetic manipulation of specific populations.
(© 2024. The Author(s).)
التعليقات: Update of: bioRxiv. 2024 May 26:2024.05.26.595949. doi: 10.1101/2024.05.26.595949. (PMID: 38826289)
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معلومات مُعتمدة: I01 BX004820 United States BX BLRD VA; R21 DA055846 United States DA NIDA NIH HHS; R01 DA037897 United States DA NIDA NIH HHS; R21 DA057458 United States DA NIDA NIH HHS; DP1 DA054394 United States DA NIDA NIH HHS; K01 AA028292 United States AA NIAAA NIH HHS; R21 DA045792 United States DA NIDA NIH HHS; Nonformula Tobacco Settlement Act Grant Pennsylvania Department of Health; T32IR5226 Tobacco-Related Disease Research Program; R01 AA030056 United States AA NIAAA NIH HHS
فهرسة مساهمة: Keywords: Medium spiny neurons; Nucleus accumbens; Rat; Single cell transcriptomics
تواريخ الأحداث: Date Created: 20240806 Date Completed: 20240806 Latest Revision: 20240813
رمز التحديث: 20240813
مُعرف محوري في PubMed: PMC11303397
DOI: 10.1038/s41598-024-69255-0
PMID: 39107568
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-024-69255-0