دورية أكاديمية
Real-world diagnostic potential of bacterial biomarkers of canine periodontitis.
| العنوان: | Real-world diagnostic potential of bacterial biomarkers of canine periodontitis. |
|---|---|
| المؤلفون: | Wallis CV; Waltham Petcare Science Institute, Melton Mowbray, Leicestershire, United Kingdom., Soltero-Rivera M; Department of Surgical and Radiological Sciences, University of California, Davis, CA, United States., Harvey C; Colin Harvey LLC, Cherry Hill, NJ, United States., Reynolds RM; Waltham Petcare Science Institute, Melton Mowbray, Leicestershire, United Kingdom., Carvell-Miller LJ; Waltham Petcare Science Institute, Melton Mowbray, Leicestershire, United Kingdom., Colyer A; Waltham Petcare Science Institute, Melton Mowbray, Leicestershire, United Kingdom., McKee TS; VCA Clinical Studies, Los Angeles, CA, United States., Mills T; VCA Clinical Studies, Los Angeles, CA, United States., Bergman PJ; VCA Clinical Studies, Los Angeles, CA, United States., Watson P; Waltham Petcare Science Institute, Melton Mowbray, Leicestershire, United Kingdom., Holcombe LJ; Waltham Petcare Science Institute, Melton Mowbray, Leicestershire, United Kingdom. |
| المصدر: | Frontiers in veterinary science [Front Vet Sci] 2024 Jul 23; Vol. 11, pp. 1377119. Date of Electronic Publication: 2024 Jul 23 (Print Publication: 2024). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101666658 Publication Model: eCollection Cited Medium: Print ISSN: 2297-1769 (Print) Linking ISSN: 22971769 NLM ISO Abbreviation: Front Vet Sci Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Media S.A., [2014]- |
| مستخلص: | Introduction: The objective of this study was to investigate the diagnostic potential of bacterial biomarkers by comparing the performance of molecular detection assays with clinical assessments of dog's oral health performed by veterinarians. Methods: Supragingival and subgingival plaque samples were collected from 127 client-owned dogs, pre-booked for procedures under general anesthesia, visiting veterinary practices in the United States. DNA was extracted and bacterial biomarkers quantified using quantitative polymerase chain reaction. Gingivitis and periodontitis were recorded by a trained clinician using the Weighted Gingivitis Periodontitis Score which involved assessing the buccal surfaces of 18 teeth while under general anesthesia. Intraoral dental radiographs of the left and right mandibular first molar teeth were also obtained. These data were then used to establish the diagnostic performance of the molecular assay to detect periodontitis. Results: An initial conscious, visual oral examination performed by the veterinarian identified 67.7% of dogs as having periodontitis, but examination under general anesthesia indicated a higher proportion (86.6%). Analysis of supragingival plaque samples collected by veterinarians from conscious and unconscious dogs demonstrated the assay had an accuracy of 77.7 to 80.9%, a sensitivity of 77.6 to 81.0%, and a specificity of 80.0%. Discussion: Use of this molecular screening tool in conscious dogs has the potential to improve early periodontal disease detection and support veterinary decision making, ultimately improving the oral health of dogs and consequently their quality of life. Competing Interests: CW, LC-M, RR, PB, TSM, TM, LH, PW, and AC are employees of Mars Petcare, a manufacturer of commercial petfood and provider of veterinary services. Mars Petcare has filed a number of patents relating to the compositional analysis of the canine oral microbiome as a diagnostic for canine periodontal disease. CH and MS-R received payment from Mars Petcare for consultation relating to this research. CH is employed by the company Colin Harvey LLC. (Copyright This work is authored by Wallis, Soltero-Rivera, Harvey, Reynolds, Carvell-Miller, Colyer, McKee, Millis, Bergman, Watson and Holcombe. © 2024 Mars Petcare UK and its affiliates for Wallis, Reynolds, Carvell-Miller, Colyer, Watson and Holcombe and © 2024 VCA Clinical Studies for McKee, Mills and Bergman.) |
| References: | J Appl Oral Sci. 2009 Jan-Feb;17(1):. (PMID: 19148397) BMC Vet Res. 2021 Jan 6;17(1):7. (PMID: 33407419) J Small Anim Pract. 2020 Sep;61(9):529-540. (PMID: 32955734) J Small Anim Pract. 2008 Dec;49(12):610-6. (PMID: 18793256) NPJ Biofilms Microbiomes. 2017 Jan 26;3:2. (PMID: 28649403) J Vet Dent. 1994 Oct;11(3):94-105. (PMID: 9693607) J Am Vet Med Assoc. 2009 Feb 15;234(4):486-94. (PMID: 19222358) Nihon Juigaku Zasshi. 1989 Dec;51(6):1151-62. (PMID: 2601227) PLoS One. 2014 Mar 04;9(3):e90501. (PMID: 24594665) J Periodontal Res. 2012 Jun;47(3):354-64. (PMID: 22181039) PLoS One. 2012;7(4):e36067. (PMID: 22558330) Vet Clin North Am Small Anim Pract. 2022 Jan;52(1):121-137. (PMID: 34838247) BMC Vet Res. 2023 Sep 18;19(1):163. (PMID: 37723566) PLoS One. 2022 Jan 21;17(1):e0262859. (PMID: 35061858) J Am Vet Med Assoc. 1999 May 1;214(9):1336-41. (PMID: 10319174) Vet Clin North Am Small Anim Pract. 1998 Sep;28(5):1111-28, vi. (PMID: 9779543) Res Vet Sci. 2021 May;136:396-407. (PMID: 33799170) J Clin Microbiol. 2005 Nov;43(11):5470-6. (PMID: 16272472) J Vet Dent. 2008 Sep;25(3):176-80. (PMID: 19025138) J Small Anim Pract. 1996 Jul;37(7):309-16. (PMID: 8840250) J Periodontal Res. 1975 Nov;10(5):243-55. (PMID: 129548) J Vet Dent. 2012 Winter;29(4):222-6. (PMID: 23505784) Am J Vet Res. 2021 Nov 01;83(1):42-49. (PMID: 34727047) Res Vet Sci. 2017 Oct;114:469-473. (PMID: 28802947) Vet Microbiol. 2015 Dec 31;181(3-4):271-82. (PMID: 26507828) PLoS One. 2014 Dec 02;9(12):e113744. (PMID: 25463050) Can Vet J. 2011 Dec;52(12):1361-2. (PMID: 22654147) Biom J. 2008 Jun;50(3):346-63. (PMID: 18481363) J Vet Dent. 2008 Jun;25(2):97-105. (PMID: 18751659) Can Vet J. 2012 Dec;53(12):1260-5. (PMID: 23729822) PLoS One. 2013 Dec 13;8(12):e83158. (PMID: 24349448) BMC Vet Res. 2019 Jun 21;15(1):207. (PMID: 31226991) J Am Anim Hosp Assoc. 2018 May/Jun;54(3):132-137. (PMID: 29558213) Vet Microbiol. 2005 Mar 20;106(1-2):119-28. (PMID: 15737481) Bull Tokyo Dent Coll. 2008 Aug;49(3):99-106. (PMID: 19129684) J Int Acad Periodontol. 2005 Jan;7(1):3-7. (PMID: 15736889) J Vet Dent. 1996 Jun;13(2):57-60. (PMID: 9520780) Am J Vet Res. 1998 Jun;59(6):686-91. (PMID: 9622735) Ann Intern Med. 2004 Feb 3;140(3):189-202. (PMID: 14757617) J Am Vet Med Assoc. 2012 Feb 15;240(4):427-36. (PMID: 22309015) J Small Anim Pract. 2014 Mar;55(3):160-3. (PMID: 24450418) J Periodontal Res. 1980 Jul;15(4):380-9. (PMID: 6449573) Am J Vet Res. 2021 Nov 01;83(1):50-58. (PMID: 34727048) Vet J. 2021 Sep;275:105717. (PMID: 34293444) Vet Sci. 2021 Nov 27;8(12):. (PMID: 34941818) J Clin Epidemiol. 2009 Jan;62(1):5-12. (PMID: 18778913) |
| فهرسة مساهمة: | Keywords: detection; diagnostic; dog; microbiota; periodontal disease |
| تواريخ الأحداث: | Date Created: 20240807 Latest Revision: 20240808 |
| رمز التحديث: | 20240808 |
| مُعرف محوري في PubMed: | PMC11301947 |
| DOI: | 10.3389/fvets.2024.1377119 |
| PMID: | 39109343 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2297-1769 |
|---|---|
| DOI: | 10.3389/fvets.2024.1377119 |