دورية أكاديمية
Protein profile of purified plasma- and aqueous humor-derived extracellular vesicles from patients with ocular toxoplasmosis.
| العنوان: | Protein profile of purified plasma- and aqueous humor-derived extracellular vesicles from patients with ocular toxoplasmosis. |
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| المؤلفون: | Costa DF; MUHC-McGill University Ocular Pathology & Translational Research Laboratory Montreal, Canada.; Department of Ophthalmology, Universidade Federal de São Paulo, São Paulo, SP, Brazil., Pessuti CL; Department of Ophthalmology, Universidade Federal de São Paulo, São Paulo, SP, Brazil., Tsering T; MUHC-McGill University Ocular Pathology & Translational Research Laboratory Montreal, Canada., Abdouh M; MUHC-McGill University Ocular Pathology & Translational Research Laboratory Montreal, Canada., Ribeiro K; Department of Ophthalmology, Universidade Federal de São Paulo, São Paulo, SP, Brazil., Nascimento H; Department of Ophthalmology, Universidade Federal de São Paulo, São Paulo, SP, Brazil., Commodaro AG; Department of Ophthalmology, Universidade Federal de São Paulo, São Paulo, SP, Brazil., Burnier JV; Cancer Research Program, Research Institute of McGill University Health Centre, Montreal, Canada., Belfort R Jr; Department of Ophthalmology, Universidade Federal de São Paulo, São Paulo, SP, Brazil., Burnier MN Jr; MUHC-McGill University Ocular Pathology & Translational Research Laboratory Montreal, Canada. |
| المصدر: | Arquivos brasileiros de oftalmologia [Arq Bras Oftalmol] 2024 Aug 02; Vol. 88 (1), pp. e20230037. Date of Electronic Publication: 2024 Aug 02 (Print Publication: 2024). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Conselho Brasileiro de Oftalmologia Country of Publication: Brazil NLM ID: 0400645 Publication Model: eCollection Cited Medium: Internet ISSN: 1678-2925 (Electronic) Linking ISSN: 00042749 NLM ISO Abbreviation: Arq Bras Oftalmol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: São Paulo : Conselho Brasileiro de Oftalmologia Original Publication: São Paulo. |
| مواضيع طبية MeSH: | Aqueous Humor*/metabolism , Aqueous Humor*/chemistry , Aqueous Humor*/parasitology , Extracellular Vesicles*/metabolism , Toxoplasmosis, Ocular* , Cataract*/metabolism , Blotting, Western*, Humans ; Male ; Female ; Middle Aged ; Adult ; Tetraspanin 30/analysis ; Tetraspanin 30/metabolism ; Mass Spectrometry ; Aged ; DNA-Binding Proteins ; Transcription Factors ; Endosomal Sorting Complexes Required for Transport |
| مستخلص: | Purpose: To characterize the extracellular vesicle protein cargo in the aqueous humor and plasma of patients with ocular toxoplasmosis. Methods: Aqueous humor and plasma were collected from six patients with active ocular toxoplasmosis and six patients with cataract. Extracellular vesicles were isolated, and western blotting and mass spectrometry were performed for protein analysis. Results: All plasma samples from patients with ocular toxoplasmosis and cataract were positive for the tetraspanins CD63 and TSG101. However, the aqueous humor from patients with ocular toxoplasmosis was positive only for CD63. Sixty-seven new unreported proteins were identified in the aqueous humor and plasma of patients with the ocular toxoplasmosis and cataract. Of the 67 proteins, 10 and 7 were found only in the cataract and ocular toxoplasmosis groups, respectively. In general, these proteins were involved in immune system activation and retina homeostasis and were related to infections and retina-associated diseases. Conclusion: The distinct protein signatures between ocular toxoplasmosis and cataract may be helpful in the differential diagnosis of ocular toxoplasmosis. However, more studies are needed to better understand the role of these proteins in the pathogenesis of ocular toxoplasmosis. |
| المشرفين على المادة: | 0 (Tetraspanin 30) 0 (Tsg101 protein) 0 (DNA-Binding Proteins) 0 (Transcription Factors) 0 (Endosomal Sorting Complexes Required for Transport) |
| تواريخ الأحداث: | Date Created: 20240807 Date Completed: 20240807 Latest Revision: 20240807 |
| رمز التحديث: | 20240807 |
| DOI: | 10.5935/0004-2749.2023-0037 |
| PMID: | 39109736 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1678-2925 |
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| DOI: | 10.5935/0004-2749.2023-0037 |