دورية أكاديمية
أعرض في EDS

Short-peptide-based enteral nutrition affects rats MDP translocation and protects against gut-lung injury via the PepT1-NOD2-beclin-1 pathway in vivo.

التفاصيل البيبلوغرافية
العنوان: Short-peptide-based enteral nutrition affects rats MDP translocation and protects against gut-lung injury via the PepT1-NOD2-beclin-1 pathway in vivo.
المؤلفون: Pang XF; Department of Emergency Intensive Care Unit, Yangpu Hospital, School of Medicine, Tongji University, No. 450, Tengyue Road, Shanghai, 200090, China., Dai XY; Department of Emergency Intensive Care Unit, Yangpu Hospital, School of Medicine, Tongji University, No. 450, Tengyue Road, Shanghai, 200090, China., Zhao LJ; Department of Geriatrics, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, 200092, China., Ye YW; Department of Emergency Intensive Care Unit, Yangpu Hospital, School of Medicine, Tongji University, No. 450, Tengyue Road, Shanghai, 200090, China., Yang XY; Department of Emergency Intensive Care Unit, Yangpu Hospital, School of Medicine, Tongji University, No. 450, Tengyue Road, Shanghai, 200090, China., Wang HH; Department of Emergency Intensive Care Unit, Yangpu Hospital, School of Medicine, Tongji University, No. 450, Tengyue Road, Shanghai, 200090, China., Jiang M; Department of Emergency Intensive Care Unit, Yangpu Hospital, School of Medicine, Tongji University, No. 450, Tengyue Road, Shanghai, 200090, China., Zhu YQ; Department of Emergency Intensive Care Unit, Yangpu Hospital, School of Medicine, Tongji University, No. 450, Tengyue Road, Shanghai, 200090, China., Shi B; Department of Emergency Intensive Care Unit, Yangpu Hospital, School of Medicine, Tongji University, No. 450, Tengyue Road, Shanghai, 200090, China. joysb1969@sina.com.
المصدر: Molecular biology reports [Mol Biol Rep] 2024 Aug 07; Vol. 51 (1), pp. 891. Date of Electronic Publication: 2024 Aug 07.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Reidel Country of Publication: Netherlands NLM ID: 0403234 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-4978 (Electronic) Linking ISSN: 03014851 NLM ISO Abbreviation: Mol Biol Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Dordrecht, Boston, Reidel.
مواضيع طبية MeSH: Peptide Transporter 1*/metabolism , Peptide Transporter 1*/genetics , Beclin-1*/metabolism , Beclin-1*/genetics , Rats, Sprague-Dawley* , Nod2 Signaling Adaptor Protein*/metabolism , Nod2 Signaling Adaptor Protein*/genetics , Signal Transduction*/drug effects , Lung Injury*/metabolism , Acetylmuramyl-Alanyl-Isoglutamine*/pharmacology , Enteral Nutrition*/methods, Animals ; Rats ; Male ; Apoptosis/drug effects ; Intestinal Mucosa/metabolism ; Intestinal Mucosa/drug effects ; Intestinal Mucosa/pathology ; Autophagy/drug effects ; Lung/metabolism ; Lung/pathology ; Lung/drug effects ; Inflammation/metabolism
مستخلص: Background: Peptide transporter 1 (PepT1) transports bacterial oligopeptide products and induces inflammation of the bowel. Nutritional peptides compete for the binding of intestinal bacterial products to PepT1. We investigated the mechanism of short-peptide-based enteral nutrition (SPEN) on the damage to the gut caused by the bacterial oligopeptide product muramyl dipeptide (MDP), which is transported by PepT1. The gut-lung axis is a shared mucosal immune system, and immune responses and disorders can affect the gut-respiratory relationship.
Methods and Results: Sprague-Dawley rats were gavaged with solutions containing MDP, MDP + SPEN, MDP + intact-protein-based enteral nutrition (IPEN), glucose as a control, or glucose with GSK669 (a NOD2 antagonist). Inflammation, mitochondrial damage, autophagy, and apoptosis were explored to determine the role of the PepT1-nucleotide-binding oligomerization domain-containing protein 2 (NOD2)-beclin-1 signaling pathway in the small intestinal mucosa. MDP and proinflammatory factors of lung tissue were explored to determine that MDP can migrate to lung tissue and cause inflammation. Induction of proinflammatory cell accumulation and intestinal damage in MDP gavage rats was associated with increased NOD2 and Beclin-1 mRNA expression. IL-6 and TNF-α expression and apoptosis were increased, and mitochondrial damage was severe, as indicated by increased mtDNA in the MDP group compared with controls. MDP levels and expression of proinflammatory factors in lung tissue increased in the MDP group compared with the control group. SPEN, but not IPEN, eliminated these impacts.
Conclusions: Gavage of MDP to rats resulted in damage to the gut-lung axis. SPEN reverses the adverse effects of MDP. The PepT1-NOD2-beclin-1 pathway plays a role in small intestinal inflammation, mitochondrial damage, autophagy, and apoptosis.
(© 2024. The Author(s).)
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معلومات مُعتمدة: Z-2017-24-2026 China International Medical Foundation; 202240064 Research Fund of Shanghai Municipal Health Commission
فهرسة مساهمة: Keywords: Apoptosis; Autophagy; Inflammation; Mitochondria; Muramyl dipeptide (MDP); Peptides
المشرفين على المادة: 0 (Peptide Transporter 1)
0 (Beclin-1)
0 (Nod2 Signaling Adaptor Protein)
0 (NOD2 protein, rat)
53678-77-6 (Acetylmuramyl-Alanyl-Isoglutamine)
0 (Slc15a1 protein, rat)
0 (Becn1 protein, rat)
تواريخ الأحداث: Date Created: 20240807 Date Completed: 20240807 Latest Revision: 20240810
رمز التحديث: 20240812
مُعرف محوري في PubMed: PMC11306270
DOI: 10.1007/s11033-024-09759-0
PMID: 39110355
قاعدة البيانات: MEDLINE
الوصف
تدمد:1573-4978
DOI:10.1007/s11033-024-09759-0