دورية أكاديمية
أعرض في EDS

A broad-spectrum multiepitope vaccine against seasonal influenza A and B viruses in mice.

التفاصيل البيبلوغرافية
العنوان: A broad-spectrum multiepitope vaccine against seasonal influenza A and B viruses in mice.
المؤلفون: Yuan L; School of Public Health (Shenzhen), Shenzhen Key Laboratory of Pathogenic Microbes and Biosafetuy, Shenzhen Campus of Sun Yat-sen University, Shenzhen, 518107, PR China. Electronic address: yuanlf6@mail2.sysu.edu.cn., Zhang S; School of Public Health (Shenzhen), Shenzhen Key Laboratory of Pathogenic Microbes and Biosafetuy, Shenzhen Campus of Sun Yat-sen University, Shenzhen, 518107, PR China. Electronic address: zhangshz5@mail2.sysu.edu.cn., Bi R; School of Public Health (Shenzhen), Shenzhen Key Laboratory of Pathogenic Microbes and Biosafetuy, Shenzhen Campus of Sun Yat-sen University, Shenzhen, 518107, PR China. Electronic address: birj@mail2.sysu.edu.cn., Liu X; School of Public Health (Shenzhen), Shenzhen Key Laboratory of Pathogenic Microbes and Biosafetuy, Shenzhen Campus of Sun Yat-sen University, Shenzhen, 518107, PR China. Electronic address: liuxj89@mail2.sysu.edu.cn., Han Z; School of Public Health (Shenzhen), Shenzhen Key Laboratory of Pathogenic Microbes and Biosafetuy, Shenzhen Campus of Sun Yat-sen University, Shenzhen, 518107, PR China. Electronic address: hanzr@mail2.sysu.edu.cn., Li M; School of Public Health (Shenzhen), Shenzhen Key Laboratory of Pathogenic Microbes and Biosafetuy, Shenzhen Campus of Sun Yat-sen University, Shenzhen, 518107, PR China. Electronic address: liminchao@mail2.sysu.edu.cn., Liao X; School of Public Health (Shenzhen), Shenzhen Key Laboratory of Pathogenic Microbes and Biosafetuy, Shenzhen Campus of Sun Yat-sen University, Shenzhen, 518107, PR China. Electronic address: liaoxzh8@mail2.sysu.edu.cn., Xie T; School of Public Health (Shenzhen), Shenzhen Key Laboratory of Pathogenic Microbes and Biosafetuy, Shenzhen Campus of Sun Yat-sen University, Shenzhen, 518107, PR China. Electronic address: xiet27@mail2.sysu.edu.cn., Bai S; School of Public Health (Shenzhen), Shenzhen Key Laboratory of Pathogenic Microbes and Biosafetuy, Shenzhen Campus of Sun Yat-sen University, Shenzhen, 518107, PR China. Electronic address: baishh7@mail2.sysu.edu.cn., Xie Q; School of Public Health (Shenzhen), Shenzhen Key Laboratory of Pathogenic Microbes and Biosafetuy, Shenzhen Campus of Sun Yat-sen University, Shenzhen, 518107, PR China. Electronic address: xieq59@mail.sysu.edu.cn., Luo C; School of Public Health (Shenzhen), Shenzhen Key Laboratory of Pathogenic Microbes and Biosafetuy, Shenzhen Campus of Sun Yat-sen University, Shenzhen, 518107, PR China. Electronic address: luochm7@mail.sysu.edu.cn., Jiang Y; Shenzhen Nanshan Centre for Disease Control and Prevention, Shenzhen, 518054, PR China. Electronic address: wangtiedan0111@126.com., Yuan J; Shenzhen Nanshan Centre for Disease Control and Prevention, Shenzhen, 518054, PR China. Electronic address: sncdcyjh@szns.gov.cn., Luo H; School of Public Health (Shenzhen), Shenzhen Key Laboratory of Pathogenic Microbes and Biosafetuy, Shenzhen Campus of Sun Yat-sen University, Shenzhen, 518107, PR China; Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education, Guangzhou, 510080, PR China. Electronic address: luohle@mail.sysu.edu.cn., Yan H; Centre for Disease Control and Prevention of Southern Military Theatre, 510610, Guangzhou, PR China. Electronic address: watshing@126.com., Sun C; School of Public Health (Shenzhen), Shenzhen Key Laboratory of Pathogenic Microbes and Biosafetuy, Shenzhen Campus of Sun Yat-sen University, Shenzhen, 518107, PR China; Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education, Guangzhou, 510080, PR China. Electronic address: suncaijun@mail.sysu.edu.cn., Shu Y; School of Public Health (Shenzhen), Shenzhen Key Laboratory of Pathogenic Microbes and Biosafetuy, Shenzhen Campus of Sun Yat-sen University, Shenzhen, 518107, PR China; Key Laboratory of Pathogen Infection Prevention and Control (MOE), State Key Laboratory of Respiratory Health and Multimorbidity, National Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 102629, PR China. Electronic address: shuylong@mail.sysu.edu.cn.
المصدر: EBioMedicine [EBioMedicine] 2024 Aug; Vol. 106, pp. 105269. Date of Electronic Publication: 2024 Aug 06.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier B.V Country of Publication: Netherlands NLM ID: 101647039 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2352-3964 (Electronic) Linking ISSN: 23523964 NLM ISO Abbreviation: EBioMedicine Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Amsterdam] : Elsevier B.V., [2014]-
مواضيع طبية MeSH: Influenza Vaccines*/immunology , Influenza Vaccines*/administration & dosage , Influenza B virus*/immunology , Orthomyxoviridae Infections*/prevention & control , Orthomyxoviridae Infections*/immunology , Epitopes, B-Lymphocyte*/immunology, Animals ; Mice ; Female ; Influenza A virus/immunology ; Antibodies, Viral/immunology ; Epitopes, T-Lymphocyte/immunology ; Disease Models, Animal ; Mice, Inbred C57BL ; Vaccines, DNA/immunology ; Vaccines, DNA/administration & dosage ; Seasons ; Influenza A Virus, H3N2 Subtype/immunology ; Humans
مستخلص: Background: Influenza viruses pose a persistent threat to global public health, necessitating the development of innovative and broadly effective vaccines.
Methods: This study focuses on a multiepitope vaccine (MEV) designed to provide broad-spectrum protection against different influenza viruses. The MEV, containing 19 B-cell linear epitopes, 7 CD4 + T cells, and 11 CD8 + T cells epitopes identified through enzyme-linked immunospot assay (ELISPOT) in influenza viruses infected mice, was administered through a regimen of two doses of DNA vaccine followed by one dose of a protein vaccine in C57BL/6 female mice.
Findings: Upon lethal challenge with both seasonal circulating strains (H1N1, H3N2, BV, and BY) and historical strains (H1N1-PR8 and H3N2-X31), MEV demonstrated substantial protection against different influenza seasonal strains, with partial efficacy against historical strains. Notably, the increased germinal centre B cells and antibody-secreting cells, along with robust T cell immune responses, highlighted the comprehensive immune defence elicited by MEV. Elevated hemagglutinin inhibition antibody was also observed against seasonal circulating and historical strains. Additionally, mice vaccinated with MEV exhibited significantly lower counts of inflammatory cells in the lungs compared to negative control groups.
Interpretation: Our results demonstrated the efficacy of a broad-spectrum MEV against influenza viruses in mice. Conducting long-term studies to evaluate the durability of MEV-induced immune responses and explore its potential application in diverse populations will offer valuable insights for the continued advancement of this promising vaccine.
Funding: Funding bodies are described in the Acknowledgments section.
Competing Interests: Declaration of interests The authors declare that they have no competing financial interests.
(Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Broad-spectrum vaccine; Influenza; Multiepitope; Peptide; Peptide-based vaccine
المشرفين على المادة: 0 (Influenza Vaccines)
0 (Epitopes, B-Lymphocyte)
0 (Antibodies, Viral)
0 (Epitopes, T-Lymphocyte)
0 (Vaccines, DNA)
تواريخ الأحداث: Date Created: 20240807 Date Completed: 20240816 Latest Revision: 20240816
رمز التحديث: 20240818
DOI: 10.1016/j.ebiom.2024.105269
PMID: 39111250
قاعدة البيانات: MEDLINE
الوصف
تدمد:2352-3964
DOI:10.1016/j.ebiom.2024.105269