A spatial expression atlas of the adult human proximal small intestine.

التفاصيل البيبلوغرافية
العنوان:	A spatial expression atlas of the adult human proximal small intestine.
المؤلفون:	Harnik Y; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel., Yakubovsky O; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.; Department of General Surgery and Transplantation, Sheba Medical Center, Ramat Gan, Israel.; Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel., Hoefflin R; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel., Novoselsky R; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel., Bahar Halpern K; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel., Barkai T; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.; Sheba Medical Center, Ramat Gan, Israel., Korem Kohanim Y; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA., Egozi A; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel., Golani O; Department of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel., Addadi Y; Department of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel., Kedmi M; Department of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel., Keidar Haran T; Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel., Levin Y; The De Botton Institute for Protein Profiling, The Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel., Savidor A; The De Botton Institute for Protein Profiling, The Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel., Keren-Shaul H; Department of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel., Mayer C; Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.; Institute of Pathology, Sheba Medical Center, Ramat Gan, Israel., Pencovich N; Department of General Surgery and Transplantation, Sheba Medical Center, Ramat Gan, Israel.; Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel., Pery R; Department of General Surgery and Transplantation, Sheba Medical Center, Ramat Gan, Israel.; Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel., Shouval DS; Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.; Institute of Gastroenterology, Nutrition and Liver Diseases, Schneider Children's Medical Center of Israel, Petach Tikva, Israel., Tirosh I; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel., Nachmany I; Department of General Surgery and Transplantation, Sheba Medical Center, Ramat Gan, Israel.; Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel., Itzkovitz S; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel. shalev.itzkovitz@weizmann.ac.il.
المصدر:	Nature [Nature] 2024 Aug; Vol. 632 (8027), pp. 1101-1109. Date of Electronic Publication: 2024 Aug 07.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Nature Publishing Group Country of Publication: England NLM ID: 0410462 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-4687 (Electronic) Linking ISSN: 00280836 NLM ISO Abbreviation: Nature Subsets: MEDLINE
أسماء مطبوعة:	Publication: Basingstoke : Nature Publishing Group Original Publication: London, Macmillan Journals ltd.
مواضيع طبية MeSH:	Gene Expression Profiling* , Intestine, Small/cytology , Intestine, Small/immunology , Intestine, Small/metabolism , Cell Biology, Adult ; Animals ; Female ; Humans ; Male ; Mice ; Cell Movement ; Chylomicrons/biosynthesis ; Enterocytes/metabolism ; Enterocytes/cytology ; Epithelial Cells ; In Situ Hybridization, Fluorescence ; Intestinal Mucosa/cytology ; Intestinal Mucosa/immunology ; Intestinal Mucosa/metabolism ; Iron/metabolism ; Lipid Droplets/metabolism ; Macrophages/cytology ; Macrophages/immunology ; Macrophages/metabolism ; Mesoderm/cytology ; Mesoderm/metabolism ; Proteomics ; Single Molecule Imaging ; T-Lymphocytes/cytology ; T-Lymphocytes/immunology ; T-Lymphocytes/metabolism ; Transcriptome
مستخلص:	The mouse small intestine shows profound variability in gene expression along the crypt-villus axis ^1,2 . Whether similar spatial heterogeneity exists in the adult human gut remains unclear. Here we use spatial transcriptomics, spatial proteomics and single-molecule fluorescence in situ hybridization to reconstruct a comprehensive spatial expression atlas of the adult human proximal small intestine. We describe zonated expression and cell type representation for epithelial, mesenchymal and immune cell types. We find that migrating enterocytes switch from lipid droplet assembly and iron uptake at the villus bottom to chylomicron biosynthesis and iron release at the tip. Villus tip cells are pro-immunogenic, recruiting γδ T cells and macrophages to the tip, in contrast to their immunosuppressive roles in mouse. We also show that the human small intestine contains abundant serrated and branched villi that are enriched at the tops of circular folds. Our study presents a detailed resource for understanding the biology of the adult human small intestine. (© 2024. The Author(s), under exclusive licence to Springer Nature Limited.)
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المشرفين على المادة:	0 (Chylomicrons) E1UOL152H7 (Iron)
تواريخ الأحداث:	Date Created: 20240807 Date Completed: 20240829 Latest Revision: 20240917
رمز التحديث:	20240918
DOI:	10.1038/s41586-024-07793-3
PMID:	39112711
قاعدة البيانات:	MEDLINE

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تدمد:	1476-4687
DOI:	10.1038/s41586-024-07793-3