دورية أكاديمية
Bright New Resources for Syphilis Research: Genetically Encoded Fluorescent Tags for Treponema pallidum and Sf1Ep Cells.
| العنوان: | Bright New Resources for Syphilis Research: Genetically Encoded Fluorescent Tags for Treponema pallidum and Sf1Ep Cells. |
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| المؤلفون: | Grillová L; Parasites and Microbes Programme, Wellcome Sanger Institute, Hinxton, UK., Romeis E; Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, Washington, USA., Lieberman NAP; Department of Laboratory Medicine and Pathology, University of Washington, Seattle, Washington, USA., Tantalo LC; Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, Washington, USA., Xu LH; Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, Washington, USA., Molini B; Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, Washington, USA., Trejos AT; Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, Washington, USA.; Department of Global Health, University of Washington, Seattle, Washington, USA., Lacey G; Parasites and Microbes Programme, Wellcome Sanger Institute, Hinxton, UK., Goulding D; Parasites and Microbes Programme, Wellcome Sanger Institute, Hinxton, UK., Thomson NR; Parasites and Microbes Programme, Wellcome Sanger Institute, Hinxton, UK.; Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, UK., Greninger AL; Department of Laboratory Medicine and Pathology, University of Washington, Seattle, Washington, USA.; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, Washington, USA., Giacani L; Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, Washington, USA.; Department of Global Health, University of Washington, Seattle, Washington, USA. |
| المصدر: | Molecular microbiology [Mol Microbiol] 2024 Aug 08. Date of Electronic Publication: 2024 Aug 08. |
| Publication Model: | Ahead of Print |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 8712028 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2958 (Electronic) Linking ISSN: 0950382X NLM ISO Abbreviation: Mol Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Oxford, OX ; Boston, MA : Blackwell Scientific Publications, c1987- |
| مستخلص: | The recently discovered methodologies to cultivate and genetically manipulate Treponema pallidum subsp. pallidum (T. pallidum) have significantly helped syphilis research, allowing the in vitro evaluation of antibiotic efficacy, performance of controlled studies to assess differential treponemal gene expression, and generation of loss-of-function mutants to evaluate the contribution of specific genetic loci to T. pallidum virulence. Building on this progress, we engineered the T. pallidum SS14 strain to express a red-shifted green fluorescent protein (GFP) and Sf1Ep cells to express mCherry and blue fluorescent protein (BFP) for enhanced visualization. These new resources improve microscopy- and cell sorting-based applications for T. pallidum, better capturing the physical interaction between the host and pathogen, among other possibilities. Continued efforts to develop and share new tools and resources are required to help our overall knowledge of T. pallidum biology and syphilis pathogenesis reach that of other bacterial pathogens, including spirochetes. (© 2024 The Author(s). Molecular Microbiology published by John Wiley & Sons Ltd.) |
| التعليقات: | Update of: bioRxiv. 2024 May 29:2024.05.29.596454. doi: 10.1101/2024.05.29.596454. (PMID: 38854070) |
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| معلومات مُعتمدة: | 8394150 Open Philanthropy Project; U19AI144133 National Institute of Allergy and Infectious Diseases; BB/010589 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; 206194 United Kingdom WT_ Wellcome Trust; INV-051483 the Bill & Melinda Gates Foundation |
| فهرسة مساهمة: | Keywords: GFP‐expressing Treponema pallidum subsp. pallidum; fluorescence microscopy; genetic manipulation; mCherry and BFP‐expressing Sf1Ep cells |
| تواريخ الأحداث: | Date Created: 20240808 Latest Revision: 20240813 |
| رمز التحديث: | 20240813 |
| DOI: | 10.1111/mmi.15304 |
| PMID: | 39115038 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1365-2958 |
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| DOI: | 10.1111/mmi.15304 |