دورية أكاديمية
أعرض في EDS

Nesprin-2 coordinates opposing microtubule motors during nuclear migration in neurons.

التفاصيل البيبلوغرافية
العنوان: Nesprin-2 coordinates opposing microtubule motors during nuclear migration in neurons.
المؤلفون: Zhou C; Graduate School of Biostudies, Kyoto University, Kyoto, Japan.; Institute for Integrated Cell-Material Science (WPI-iCeMS), Kyoto University, Kyoto, Japan., Wu YK; Graduate School of Biostudies, Kyoto University, Kyoto, Japan.; Institute for Integrated Cell-Material Science (WPI-iCeMS), Kyoto University, Kyoto, Japan., Ishidate F; Institute for Integrated Cell-Material Science (WPI-iCeMS), Kyoto University, Kyoto, Japan., Fujiwara TK; Institute for Integrated Cell-Material Science (WPI-iCeMS), Kyoto University, Kyoto, Japan., Kengaku M; Graduate School of Biostudies, Kyoto University, Kyoto, Japan.; Institute for Integrated Cell-Material Science (WPI-iCeMS), Kyoto University, Kyoto, Japan.
المصدر: The Journal of cell biology [J Cell Biol] 2024 Nov 04; Vol. 223 (11). Date of Electronic Publication: 2024 Aug 08.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Rockefeller University Press Country of Publication: United States NLM ID: 0375356 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1540-8140 (Electronic) Linking ISSN: 00219525 NLM ISO Abbreviation: J Cell Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: New York : Rockefeller University Press
مواضيع طبية MeSH: Microtubules*/metabolism , Neurons*/metabolism , Kinesins*/metabolism , Kinesins*/genetics , Nerve Tissue Proteins*/metabolism , Nerve Tissue Proteins*/genetics , Dyneins*/metabolism , Cell Nucleus*/metabolism , Microtubule-Associated Proteins*/metabolism , Microtubule-Associated Proteins*/genetics, Animals ; Mice ; Active Transport, Cell Nucleus ; Dynactin Complex/metabolism ; Dynactin Complex/genetics ; Cell Movement ; Microfilament Proteins/metabolism ; Microfilament Proteins/genetics ; Nuclear Proteins/metabolism ; Nuclear Proteins/genetics ; Cerebellum/metabolism ; Cerebellum/cytology ; Binding Sites ; Humans
مستخلص: Nuclear migration is critical for the proper positioning of neurons in the developing brain. It is known that bidirectional microtubule motors are required for nuclear transport, yet the mechanism of the coordination of opposing motors is still under debate. Using mouse cerebellar granule cells, we demonstrate that Nesprin-2 serves as a nucleus-motor adaptor, coordinating the interplay of kinesin-1 and dynein. Nesprin-2 recruits dynein-dynactin-BicD2 independently of the nearby kinesin-binding LEWD motif. Both motor binding sites are required to rescue nuclear migration defects caused by the loss of function of Nesprin-2. In an intracellular cargo transport assay, the Nesprin-2 fragment encompassing the motor binding sites generates persistent movements toward both microtubule minus and plus ends. Nesprin-2 drives bidirectional cargo movements over a prolonged period along perinuclear microtubules, which advance during the migration of neurons. We propose that Nesprin-2 keeps the nucleus mobile by coordinating opposing motors, enabling continuous nuclear transport along advancing microtubules in migrating cells.
(© 2024 Zhou et al.)
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معلومات مُعتمدة: 20H00483 Japan Society for the Promotion of Science; Takeda Science Foundation; JST SPRING program
المشرفين على المادة: EC 3.6.4.4 (Kinesins)
0 (Nerve Tissue Proteins)
EC 3.6.4.2 (Dyneins)
0 (Microtubule-Associated Proteins)
0 (Syne2 protein, mouse)
0 (Bicd2 protein, mouse)
0 (Dynactin Complex)
0 (Kns2 protein, mouse)
0 (Microfilament Proteins)
0 (Nuclear Proteins)
تواريخ الأحداث: Date Created: 20240808 Date Completed: 20240808 Latest Revision: 20240912
رمز التحديث: 20240912
مُعرف محوري في PubMed: PMC11310688
DOI: 10.1083/jcb.202405032
PMID: 39115447
قاعدة البيانات: MEDLINE
الوصف
تدمد:1540-8140
DOI:10.1083/jcb.202405032