دورية أكاديمية
Analysis of total microcystins by Lemieux oxidation and liquid chromatography-mass spectrometry in fish and mussels tissues: Optimization and comparison of protocols.
| العنوان: | Analysis of total microcystins by Lemieux oxidation and liquid chromatography-mass spectrometry in fish and mussels tissues: Optimization and comparison of protocols. |
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| المؤلفون: | Bouteiller P; Université de Reims Champagne-Ardenne, UMR-I 02 INERIS-URCA-ULH SEBIO, Unité Stress Environnementaux et BIOsurveillance des Milieux Aquatiques (SEBIO), BP 1039 F, 51687 Reims Cedex, France; ANSES, Laboratory for Food Safety, F-94701 Maisons-Alfort, France., Biré R; ANSES, Laboratory for Food Safety, F-94701 Maisons-Alfort, France., Foss AJ; GreenWater Laboratories/CyanoLab, 205 Zeagler Drive, Palatka, FL 32177, USA., Guérin T; ANSES, Strategy and Programmes Department, F-94701 Maisons-Alfort, France., Lance E; Université de Reims Champagne-Ardenne, UMR-I 02 INERIS-URCA-ULH SEBIO, Unité Stress Environnementaux et BIOsurveillance des Milieux Aquatiques (SEBIO), BP 1039 F, 51687 Reims Cedex, France. Electronic address: emilie.lance@univ-reims.fr. |
| المصدر: | The Science of the total environment [Sci Total Environ] 2024 Nov 10; Vol. 950, pp. 175339. Date of Electronic Publication: 2024 Aug 06. |
| نوع المنشور: | Journal Article; Comparative Study |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0330500 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1026 (Electronic) Linking ISSN: 00489697 NLM ISO Abbreviation: Sci Total Environ Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier. |
| مواضيع طبية MeSH: | Microcystins*/analysis , Oxidation-Reduction*, Animals ; Chromatography, Liquid ; Fishes ; Bivalvia ; Mass Spectrometry/methods ; Liquid Chromatography-Mass Spectrometry |
| مستخلص: | Microcystins (MCs) can be detected in various matrices in two forms: a freely extractable fraction and a total (free and covalently protein-bound) fraction. Although the majority of MCs analyses are limited to the free fraction, they do not allow the analysis of all MCs variants or protein-bound forms. Other methods, known as total MCs analysis methods, enable simultaneous analysis of all MCs variants, as well as bound forms, which may be a major form of toxin accumulation in organisms. Among these techniques, the chemical oxidation method (e.g. Lemieux) allows the detection of total forms of MC (and nodularins) by oxidizing the common part to all MC and nodularins, and analyzing the resultant MMPB product (2-methyl-3-methoxy-4-phenylbutyric acid). However, the execution of this method in the context of health monitoring is challenging due to the variability of the protocols, the recoveries obtained with these protocols, and the important matrix effects associated with the method. The objectives of this study were i) to optimize an existing protocol of chemical oxidation "Lemieux1" on fresh fish fillet matrices, ii) to compare two existing protocols ("Lemieux1" and "Lemieux2"), and iii) apply Lemieux oxidation to fish fillets and livers naturally contaminated with MCs-producing cyanobacteria and to freshwater mussels contaminated with MCs in laboratories. Optimization of the "Lemieux1" protocol, in particular in the oxidation and SPE (solid phase extraction) steps improved the method's yields on the fresh fish fillet matrix (from <5 % to around 40 %). Moreover, several quantification methods have been compared through various calibration techniques (solvent calibration curve, matrix-matched calibration curve, oxidized MC-LR calibration curve and also by testing the addition of d Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024. Published by Elsevier B.V.) |
| فهرسة مساهمة: | Keywords: Cyanotoxins; LC-MS/MS analysis; Lemieux oxidation protocols; MMPB; Microcystins; Total microcystin content |
| المشرفين على المادة: | 0 (Microcystins) |
| تواريخ الأحداث: | Date Created: 20240808 Date Completed: 20240827 Latest Revision: 20240827 |
| رمز التحديث: | 20240827 |
| DOI: | 10.1016/j.scitotenv.2024.175339 |
| PMID: | 39117191 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-1026 |
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| DOI: | 10.1016/j.scitotenv.2024.175339 |