دورية أكاديمية
أعرض في EDS

Development of two recombinant vaccines against Clostridioides difficile infection and immunogenicity in pregnant sows and neonatal piglets.

التفاصيل البيبلوغرافية
العنوان: Development of two recombinant vaccines against Clostridioides difficile infection and immunogenicity in pregnant sows and neonatal piglets.
المؤلفون: Ramos CP; Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Siqueira WF; Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Viana LA; Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Cunha JLR; York Biomedical Research Institute, Department of Biology, University of York, York, UK., Fujiwara RT; Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Amarante VS; Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Souza TGV; Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Silva ROS; Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. Electronic address: rodrigo.otaviosilva@gmail.com.
المصدر: Anaerobe [Anaerobe] 2024 Aug 08; Vol. 89, pp. 102896. Date of Electronic Publication: 2024 Aug 08.
Publication Model: Ahead of Print
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Academic Press Country of Publication: England NLM ID: 9505216 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-8274 (Electronic) Linking ISSN: 10759964 NLM ISO Abbreviation: Anaerobe Subsets: MEDLINE
أسماء مطبوعة: Publication: London : Academic Press
Original Publication: London ; San Diego : Academic Press, c1995-
مستخلص: Introduction: Clostridioides difficile is the main cause of antibiotic-associated diarrhea in humans and is a major enteropathogen in several animal species. In newborn piglets, colonic lesions caused by C. difficile A and B toxins (TcdA and TcdB, respectively) cause diarrhea and significant production losses.
Objective: The present study aimed to develop two recombinant vaccines from immunogenic C-terminal fragments of TcdA and TcdB and evaluate the immune response in rabbits and in breeding sows. Two vaccines were produced: bivalent (rAB), consisting of recombinant fragments of TcdA and TcdB, and chimeric (rQAB), corresponding to the synthesis of the same fragments in a single protein. Groups of rabbits were inoculated with 10 or 50 μg of proteins adjuvanted with aluminum or 0.85 % sterile saline in a final volume of 1 mL/dose. Anti-TcdA and anti-TcdB IgG antibodies were detected in rabbits and sows immunized with both rAB and rQAB vaccines by ELISA. The vaccinated sows were inoculated intramuscularly with 20 μg/dose using a prime-boost approach.
Results: Different antibody titers (p ≤ 0.05) were observed among the vaccinated groups of sows (rAB and rQAB) and control. Additionally, newborn piglets from vaccinated sows were also positive for anti-TcdA and anti-TcdB IgGs, in contrast to control piglets (p ≤ 0.05). Immunization of sows with the rQAB vaccine conferred higher anti-TcdA and anti-TcdB responses in piglets, suggesting the superiority of this compound over rAB.
Conclusion: The synthesized recombinant proteins were capable of inducing antibody titers against C. difficile toxins A and B in sows, and were passively transferred to piglets through colostrum.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: CDI; Immune response; Swine neonatal diarrhea; Zoonosis
تواريخ الأحداث: Date Created: 20240810 Latest Revision: 20240811
رمز التحديث: 20240813
DOI: 10.1016/j.anaerobe.2024.102896
PMID: 39127403
قاعدة البيانات: MEDLINE
الوصف
تدمد:1095-8274
DOI:10.1016/j.anaerobe.2024.102896